Results 41 to 50 of about 898 (154)

Enhanced Production of (R)-3-Hydroxybutyrate Oligomers by Coexpression of Molecular Chaperones in Recombinant Escherichia coli Harboring a Polyhydroxyalkanoate Synthase Derived from Bacillus cereus YB-4

Microorganisms, 2022
The biodegradable polyester poly-(R)-3-hydroxybutyrate [P(3HB)] is synthesized by a polymerizing enzyme called polyhydroxyalkanoate (PHA) synthase and accumulates in a wide variety of bacterial cells. Recently, we demonstrated the secretory production of
Saki Goto, Yuki Miyahara, Seiichi Taguchi, Takeharu Tsuge, Ayaka Hiroe   +4 more
doaj   +1 more source

Autotrophic Production of Stable-Isotope-Labeled Arginine in Ralstonia eutropha Strain H16 [PDF]

Applied and Environmental Microbiology, 2012
ABSTRACT With the aim of improving industrial-scale production of stable-isotope (SI)-labeled arginine, we have developed a system for the heterologous production of the arginine-containing polymer cyanophycin in recombinant strains of Ralstonia eutropha under lithoautotrophic growth conditions.
Luette, S., Pohlmann, A., Zaychikov, E., Schwartz, E., Becher, J., Heumann, H., Friedrich, B.   +6 more
openaire   +3 more sources

Toward a synthetic hydrogen sensor in cyanobacteria: Functional production of an oxygen-tolerant regulatory hydrogenase in Synechocystis sp. PCC 6803

Frontiers in Microbiology, 2023
Cyanobacteria have raised great interest in biotechnology, e.g., for the sustainable production of molecular hydrogen (H2) using electrons from water oxidation. However, this is hampered by various constraints.
Franz Opel, Marvin Amadeus Itzenhäuser, Isabel Wehner, Sara Lupacchini, Lars Lauterbach, Oliver Lenz, Stephan Klähn   +6 more
doaj   +1 more source

Versatile and stable vectors for efficient gene expression in Ralstonia eutropha H16

New Biotechnology, 2014
The Gram-negative β-proteobacterium Ralstonia eutropha H16 is primarily known for polyhydroxybutyrate (PHB) production and its ability to grow chemolithoautotrophically by using CO2 and H2 as sole carbon and energy sources. The majority of metabolic engineering and heterologous expression studies conducted so far rely on a small number of suitable ...
Steffen, Gruber, Jeremias, Hagen, Helmut, Schwab, Petra, Koefinger   +3 more
openaire   +2 more sources

Catalytic properties of the isolated diaphorase fragment of the NAD-reducing [NiFe]-hydrogenase from Ralstonia eutropha. [PDF]

PLoS ONE, 2011
The NAD+-reducing soluble hydrogenase (SH) from Ralstonia eutropha H16 catalyzes the H₂-driven reduction of NAD+, as well as reverse electron transfer from NADH to H+, in the presence of O₂. It comprises six subunits, HoxHYFUI₂, and incorporates a [NiFe]
Lars Lauterbach, Zulkifli Idris, Kylie A Vincent, Oliver Lenz   +3 more
doaj   +1 more source

Hydrogen Production and Storage: Analysing Integration of Photoelectrolysis, Electron Harvesting Lignocellulose, and Atmospheric Carbon Dioxide-Fixing Biosynthesis

Energies, 2022
Green hydrogen from photocatalytic water-splitting and photocatalytic lignocellulosic reforming is a significant proposition for renewable energy storage in global net-zero policies and strategies.
Jhuma Sadhukhan, Bruno G. Pollet, Miles Seaman   +2 more
doaj   +1 more source

Spectroscopic insights into the oxygen-tolerant membrane-associated [NiFe] hydrogenase of Ralstonia eutropha H16. [PDF]

J Biol Chem, 2009
This study provides the first spectroscopic characterization of the membrane-bound oxygen-tolerant [NiFe] hydrogenase (MBH) from Ralstonia eutropha H16 in its natural environment, the cytoplasmic membrane. The H2-converting MBH is composed of a large subunit, harboring the [NiFe] active site, and a small subunit, capable in coordinating one [3Fe4S] and
Saggu M, Zebger I, Ludwig M, Lenz O, Friedrich B, Hildebrandt P, Lendzian F.   +6 more
europepmc   +4 more sources

2-Ketogluconate Kinase from Cupriavidus necator H16: Purification, Characterization, and Exploration of Its Substrate Specificity

Molecules, 2019
We have cloned, overexpressed, purified, and characterized a 2-ketogluconate kinase (2-dehydrogluconokinase, EC 2.7.1.13) from Cupriavidus necator (Ralstonia eutropha) H16.
Israel Sánchez-Moreno, Natalia Trachtmann, Sibel Ilhan, Virgil Hélaine, Marielle Lemaire, Christine Guérard-Hélaine, Georg A. Sprenger   +6 more
doaj   +1 more source

Study of Microbial Communities in the Soda Lake of Isabel Island: Identification of Polyhydroxybutyrate (PHB) Degrading Enzymes

Environmental Microbiology Reports, Volume 18, Issue 1, February 2026.
The crater lake on Isabel Island is a moderately saline soda lake that hosts microorganisms with potential biotechnological applications. We characterised the microbial communities using seven metagenomes and two genomes to identify polyhydroxybutyrate depolymerases.
Abigail Hernández‐Vázquez, Humberto Garcia‐Arellano, Rina María González‐Cervantes, Marcos López‐Pérez, Luis Mario Hernández Soto, José Abraham Canales Meza, José Félix Aguirre‐Garrido   +6 more
wiley   +1 more source

An Efficient CO2‐Upcycling Platform Based on Engineered Halomonas TD with Enhanced Acetate‐Utilizing Capacity via Adaptive Laboratory Evolution

Advanced Science, Volume 13, Issue 3, 14 January 2026.
An electrochemical system is designed to convert CO2 into CO2‐derived electrolytes (CDE), mainly containing acetate and formate. The underlying mechanism of acetate metabolism in Halomonas TD80 is explored via ALE. Engineered TD80 produced high‐yield diversified products from CDE.
Chi Wang, Ting‐Ting Chen, Yu‐Jiao Yang, Yu‐Xi Li, Yi‐Xin Chang, Yan‐Chun Xiao, Wen‐Tai Guo, Ye Zheng, Rui‐Zhe Deng, Yu‐Xiang Tian, Wei Situ, Hong‐Wei Shen, Yu Chen, Ya‐Bin Wang, Jie Xing, Hui Wang, Lin Xia, Yi‐Na Lin, Jian‐Wen Ye   +18 more
wiley   +1 more source