Frontiers in Bioengineering and Biotechnology, 2023 It is challenging to capture carbon dioxide (CO2), a major greenhouse gas in the atmosphere, due to its high chemical stability. One potential practical solution to eliminate CO2 is to convert CO2 into formate using hydrogen (H2) (CO2 hydrogenation ...
Jaehyun Cha +5 more
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Biotransformation of Eugenol to Ferulic Acid by a Recombinant Strain of Ralstonia eutropha H16 [PDF]
Applied and Environmental Microbiology, 2002 ABSTRACT The gene loci ehyAB , calA , and calB , encoding eugenol hydroxylase, coniferyl alcohol dehydrogenase, and coniferyl aldehyde dehydrogenase, respectively, which are involved in the first steps of eugenol catabolism in Pseudomonas
Jörg, Overhage +2 more
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Characterization and modification of enzymes in the 2-ketoisovalerate biosynthesis pathway of Ralstonia eutropha H16 [PDF]
Applied Microbiology and Biotechnology, 2014 2-Ketoisovalerate is an important cellular intermediate for the synthesis of branched-chain amino acids as well as other important molecules, such as pantothenate, coenzyme A, and glucosinolate. This ketoacid can also serve as a precursor molecule for the production of biofuels, pharmaceutical agents, and flavor agents in engineered organisms, such as ...
Lu, Jingnan +3 more
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Bio-Protocol, 2018 Ralstonia eutropha H16 produces and mobilizes (re-utilizes) intracellular polyhydroxybutyrate (PHB) granules during growth. This protocol describes the visualization of intracellular Nile red stained PHB granules and the quantification of PHB by gas ...
Janina Juengert +2 more
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Elucidation of beta-oxidation pathways in Ralstonia eutropha H16 by examination of global gene expression. [PDF]
J Bacteriol, 2010 ABSTRACT Ralstonia eutropha H16 is capable of growth and polyhydroxyalkanoate production on plant oils and fatty acids. However, little is known about the triacylglycerol and fatty acid degradation pathways of this bacterium. We compare whole-cell gene expression levels of R.
Brigham CJ +6 more
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Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique
Biotechnology for Biofuels, 2018 Background Ralstonia eutropha is an important bacterium for the study of polyhydroxyalkanoates (PHAs) synthesis and CO2 fixation, which makes it a potential strain for industrial PHA production and attractive host for CO2 conversion.
Bin Xiong +5 more
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An Efficient Transformation Method for the Bioplastic‐Producing “Knallgas” Bacterium Ralstonia eutropha H16 [PDF]
Biotechnology Journal, 2017 Ralstonia eutropha H16 (also known as Cupriavidus necator H16) is a Gram‐negative lithoautotrophic β‐proteobacterium with increasing biotechnological applications, including carbon capture and utilization, biopolymer synthesis, and biofuel production.
Tee, K.L. +5 more
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Efficient biochemical production of acetoin from carbon dioxide using Cupriavidus necator H16
Biotechnology for Biofuels, 2019 Background Cupriavidus necator is the best-studied knallgas (also termed hydrogen oxidizing) bacterium and provides a model organism for studying the production of the storage polymer polyhydroxybutyrate (PHB).
Carina Windhorst, Johannes Gescher
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Microbial Cell Factories, 2020 Background CO2 is fixed by all living organisms with an autotrophic metabolism, among which the Calvin–Benson–Bassham (CBB) cycle is the most important and widespread carbon fixation pathway.
Zhongkang Li +5 more
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An innovative cloning platform enables large-scale production and maturation of an oxygen-tolerant [NiFe]-hydrogenase from Cupriavidus necator in Escherichia coli. [PDF]
PLoS ONE, 2013 Expression of multiple heterologous genes in a dedicated host is a prerequisite for approaches in synthetic biology, spanning from the production of recombinant multiprotein complexes to the transfer of tailor-made metabolic pathways.
Johannes Schiffels +5 more
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