Results 81 to 90 of about 16,264 (303)

Microfluidic Hydroporation Platform for Effective Allogeneic CAR‐T Cell Production and Subsequent Functional Cytotoxicity Analysis

Advanced Materials Technologies, EarlyView.
The hydroporator platform employs controlled hydrodynamic deformation for efficient mRNA and CRISPR/Cas9 delivery into primary human T cells, enabling allogeneic CAR‐T cell manufacturing. It preserves cell functionality and drives potent gene editing, CAR expression, and tumor cytotoxicity, while feature‐based analysis links these functional outcomes ...
Soohyun Jeon, Sein Kim, Hyun‐Young Kim, Duck Cho, Sungsu Park, Seohyun Kim, Aram J. Chung   +6 more
wiley   +1 more source

Dimerisation induced formation of the active site and the identification of three metal sites in EAL-phosphodiesterases [PDF]

, 2017
The bacterial second messenger cyclic di-3′,5′-guanosine monophosphate (c-di-GMP) is a key regulator of bacterial motility and virulence. As high levels of c-di-GMP are associated with the biofilm lifestyle, c-di-GMP hydrolysing phosphodiesterases (PDEs)
A Deepthi, A Sundriyal, A Tchigvintsev, A Ueda, A Vagin, A Winkler, AD Tischler, AD Tischler, AJ Schmidt, B Huang, BW Holloway, C Chan, C Romier, CD Boyd, CW Phippen, D Balasubramanian, D Bellini, DA D’Argenio, DJ Hosfield, DL Caly, EF Pettersen, F Rao, F Rao, G Kuppuraj, G Minasov, G Winter, G Winter, GL Winsor, GN Murshudov, H Ceri, H Kulasakara, H Zheng, I Ivanov, ID Hay, J Robert-Paganin, JH Merritt, K Syson, K-H Choi, KD Miner, KM Thormann, M Christen, M Valentini, M Vorachit, MD Winn, MVAS Navarro, MY Galperin, N Barraud, O Kirillina, P Aldridge, P Emsley, P Evans, PD Newell, PR Evans, R Donlan, R Paul, R Simm, R Tamayo, RM Keegan, RP Ryan, RP Ryan, RZ Liao, TRM Barends, TT Hoang, U Römling, V Stelitano, VB Chen, W Grzybkowski, W Kabsch, Y Li, Y Qi, Y Wang   +70 more
core   +2 more sources

Evidence for Cyclic Di-GMP-Mediated Signaling in Bacillus subtilis [PDF]

Journal of Bacteriology, 2012
ABSTRACTCyclic di-GMP (c-di-GMP) is a second messenger that regulates diverse cellular processes in bacteria, including motility, biofilm formation, cell-cell signaling, and host colonization. Studies of c-di-GMP signaling have chiefly focused on Gram-negative bacteria.
Yun, Chen, Yunrong, Chai, Jian-hua, Guo, Richard, Losick   +3 more
openaire   +2 more sources

Additive‐Manufactured, Multifunctional Bioreactor Technology for Dynamic Culture of 3D Bioprinted Tissue Models

Advanced Materials Technologies, EarlyView.
We introduce an additively manufactured bioreactor with a perfusion flow system and integrated temperature and pH sensors for skeletal muscle tissue biofabrication. The bioreactor's performance was evaluated by assessing the viability, spreading of the myoblast cells in a printed scaffold, and contraction of the isolated murine musculi interossei ...
Lys Sprenger, Johann Schorzmann, Hsuan‐Heng Lu, Muhammad Osama, Julian Bauer, Michael Haug, Oliver Friedrich, Aldo R. Boccaccini, Frank Döpper, Sahar Salehi   +9 more
wiley   +1 more source

Dynamic Executors of Bacterial Signals: Functional Versatility and Regulatory Networks of c-di-GMP Effectors

Biomolecules
Cyclic di-GMP (c-di-GMP), a universal second messenger in bacteria, orchestrates a wide array of essential life processes. Its intracellular dynamics are meticulously regulated by diguanylate cyclases (DGCs) and phosphodiesterases (PDEs), ensuring ...
Jia Jia, Ge Yun, Bingxin Liu, Xinxin Li, Meiling Jiang, Xinlu Yu, Jing Zhang, Yufei Han, Dan Liu, Junlong Zhao, Yuanyuan Wang, Gukui Chen   +11 more
doaj   +1 more source

Diffusible Signal Factor (DSF)-dependent quorum sensing in pathogenic bacteria and its exploitation for disease control [PDF]

, 2016
Cell-to-cell signals of the Diffusible Signal Factor (DSF) family are cis-2-unsaturated fatty acids of differing chain length and branching pattern. DSF signalling has been described in diverse bacteria to include plant and human pathogens where it acts ...
Dow, J. Maxwell
core   +1 more source

Chemiluminescent Biosensors for Detection of Second Messenger Cyclic di-GMP [PDF]

ACS Chemical Biology, 2018
Bacteria colonize highly diverse and complex environments, from gastrointestinal tracts to soil and plant surfaces. This colonization process is controlled in part by the intracellular signal cyclic di-GMP, which regulates bacterial motility and biofilm formation.
Andrew B. Dippel, Wyatt A. Anderson, Robert S. Evans, Samuel Deutsch, Ming C. Hammond   +4 more
openaire   +4 more sources

rWTC‐MBTA Vaccine, Alone and Enhanced with Anti‐PD1, Elicits Immune Responses against CNS and Peripheral B‐Cell Lymphoma

Advanced Science, EarlyView.
An autologous whole‐tumor‐cell vaccine (rWTC‐MBTA) is evaluated in murine CNS lymphoma. Subcutaneous vaccination activates dendritic cells, broadens T‐cell priming, and drives lymphocyte trafficking to brain tumors, producing durable tumor control. Longitudinal bioluminescence and adoptive‐transfer assays verify CNS engagement. Combination with anti‐PD‐
Yaping Zhang, Juan Ye, Mitchell Sun, Samik Chakraborty, Alex Valenzuela, Shuran Chen, Qingfeng Xue, Wenyu Shi, Karel Pacak, Herui Wang, Zhengping Zhuang   +10 more
wiley   +1 more source

The characterization of Escherichia coli CpdB as a recombinantpProtein reveals that, besides having the expected 3´-nucleotidase and 2´,3´-cyclic mononucleotide phosphodiesterase activities, it is also active as cyclic dinucleotide phosphodiesterase [PDF]

, 2016
Endogenous cyclic diadenylate phosphodiesterase activity was accidentally detected in lysates of Escherichia coli BL21. Since this kind of activity is uncommon in Gram-negative bacteria, its identification was undertaken.
Cabezas Martín, Alicia, Cameselle Viña, José Carlos, Canales García, José, Costas Vázquez, María Jesús, López Villamizar, Iralis Mercedes, Pinto Corraliza, Rosa María, Ribeiro, João Nuno Meireles da Silva Gonçalves   +6 more
core   +8 more sources

Production of 3',3'-cGAMP by a Bdellovibrio bacteriovorus promiscuous GGDEF enzyme, Bd0367, regulates exit from prey by gliding motility.

PLoS Genetics, 2022
Bacterial second messengers are important for regulating diverse bacterial lifestyles. Cyclic di-GMP (c-di-GMP) is produced by diguanylate cyclase enzymes, named GGDEF proteins, which are widespread across bacteria.
Rebecca C Lowry, Zachary F Hallberg, Rob Till, Tyler J Simons, Ruth Nottingham, Fiona Want, R Elizabeth Sockett, Ming C Hammond, Carey Lambert   +8 more
doaj   +1 more source