Engineering a bioluminescent indicator for cyclic AMP-dependent protein kinase [PDF]
Biochemical Journal, 1991 cDNA coding for the luciferase in the firefly Photinus pyralis was amplified in vitro to generate cyclic AMP-dependent protein kinase phosphorylation sites. The DNA was transcribed and translated to generate light-emitting protein. A valine at position 217 was mutated to arginine to generate a site RRFS and the heptapeptide kemptide, the ...
G B, Sala-Newby, A K, Campbell
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Experimental Hematology & Oncology, 2022 Background The protein kinase A (PKA)/cAMP response element-binding protein (CREB) has been suggested to be related to the inhibition of the proliferation of non-small cell lung cancer (NSCLC) cells.
Jae Heun Chung +14 more
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Crystal Structures Reveal Hidden Domain Mechanics in Protein Kinase A (PKA)
Biology, 2023 Cyclic-AMP-dependent protein kinase A (PKA) is a critical enzyme involved in various signaling pathways that plays a crucial role in regulating cellular processes including metabolism, gene transcription, cell proliferation, and differentiation.
Colin L. Welsh +2 more
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Kv4.2 Phosphorylation by Cyclic AMP-dependent Protein Kinase [PDF]
Journal of Biological Chemistry, 2000 Recent evidence suggests that K(+) channels composed of Kv4.2 alpha-subunits underlie a transient current in hippocampal CA1 neurons and ventricular myocytes, and activation of the cAMP second messenger cascade has been shown to modulate this transient current. We determined if Kv4.2 alpha-subunits were directly phosphorylated by cAMP-dependent protein
A E, Anderson +5 more
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Resveratrol inhibits LPS-induced MAPKs activation via activation of the phosphatidylinositol 3-kinase pathway in murine RAW 264.7 macrophage cells. [PDF]
PLoS ONE, 2012 BACKGROUND: Resveratrol is a natural polyphenolic compound that has cardioprotective, anticancer and anti-inflammatory properties. We investigated the capacity of resveratrol to protect RAW 264.7 cells from inflammatory insults and explored mechanisms ...
Yi Zong +10 more
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Conserved and distinct modes of CREB/ATF transcription factor regulation by PP2A/B56gamma and genotoxic stress. [PDF]
PLoS ONE, 2010 Activating transcription factor 1 (ATF1) and the closely related proteins CREB (cyclic AMP resonse element binding protein) and CREM (cyclic AMP response element modulator) constitute a subfamily of bZIP transcription factors that play critical roles in ...
Naval P Shanware +5 more
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Cyclic AMP-dependent Protein Kinase in Paramecium tetraurelia
Journal of Biological Chemistry, 1989 M Hochstrasser, D L Nelson
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Cyclic AMP‐dependent protein kinase does not phosphorylate cyclic GMP‐dependent protein kinase in vitro [PDF]
FEBS Letters, 1983 The autophosphorylation reaction of purified cGMP‐dependent protein kinase has been studied. Apparent initial rates of autophosphorylation in the absence of cyclic nucleotides and in the presence of cGMP and cAMP are 0.006, 0.04, 0.4 mol. Pi incorp./min−1.mol cGMP‐kinase subunit−1.
Hofmann, F., Gensheimer, H.-P.
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Deciphering the structural basis of eukaryotic protein kinase regulation. [PDF]
PLoS Biology, 2013 Eukaryotic protein kinases (EPKs) regulate numerous signaling processes by phosphorylating targeted substrates through the highly conserved catalytic domain.
Hiruy S Meharena +7 more
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Yeast cyclic AMP‐dependent protein kinase [PDF]
FEBS Letters, 1983 We have purified cyclic AMP‐dependent protein kinase from the yeast Saccharomyces cerevisiae. The purified enzyme was inactive in the absence of cyclic AMP and displayed two protein bands on SDS gel electrophoresis. One was identified as the cAMP‐binding protein by chromatography on cAMP‐agarose. M r of the latter was 50 000 while the catalytic subunit
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