Results 121 to 130 of about 611,110 (302)

Operationalization of the African Medicines Agency: Retrospective evaluation of the continental centralized pilot procedure timelines to recommendation and national registrations

British Journal of Clinical Pharmacology, EarlyView.
Aims Regulatory inconsistency across Africa causes duplicative assessments, prolonged approvals, and delayed medical access. To inform the African Medicines Agency (AMA), the African Medicines Regulatory Harmonisation (AMRH) piloted Africa's first continental scientific evaluation of human medicinal products.
Alex Juma Ismail, Lerato Moeti, Delese Mimi Darko, Stuart Walker, Sam Salek   +4 more
wiley   +1 more source

The Cyclic Di-GMP Receptor HpoR Modulates Mycobacterial Multidrug Susceptibility by Regulating IniBAC-Mediated Envelope Permeability

Microorganisms
Isoniazid (INH) targets cell wall biosynthesis and is a potent antimycobacterial agent. Elucidating the regulatory networks that govern drug susceptibility in mycobacterial models is fundamental to understanding intrinsic resistance in pathogenic species.
Xiao Liu, Xiaocui Ling, Kun Wang, Jiachen Zheng, Hao Li, Minhao Guo, Yanzhe Ou, Jie Lu, Weihui Li   +8 more
doaj   +1 more source

Cyclic di-GMP-Responsive Transcriptional Reporter Bioassays in \u27Pseudomonas aeruginosa\u27

, 2017
3′,5′-cyclic diguanosine monophosphate (cyclic di-GMP) is a bacterial secondary messenger molecule that regulates many important cellular activities and behaviors, such as motility and biofilm formation. While mass spectrometry protocols for quantitative
Borlee, Grace I., Irie, Yasuhiko, Martin, Kevin H., Borlee, Bradley R., Sauer, Karin.editor.edthttp://id.loc.gov/vocabulary/relators/edt, SpringerLink (Online service)   +5 more
core   +1 more source

What Makes an “Ideal” Cell Line for Recombinant Adeno‐Associated Virus Production?

Biotechnology and Bioengineering, EarlyView.
Several host cell types have been used to produce rAAVs to date. Cell line‐specific traits that are beneficial in the context of rAAV manufacturing are reviewed here, with the goal of developing a consensus on the ‘ideal’ characteristics that an rAAV production host should possess (created with Biorender.com).
James Conheady, Nicholas Donohue, Alexandra Bogdanovic, Sharon Davin, Brian Glennon, Niall Barron, C. Mark Smales   +6 more
wiley   +1 more source

Effects of the signaling molecule cyclic-di-GMP on cyanobacterial circadian rhythm in Synechococcus elongatus PCC 7942

Journal of Bacteriology
In phototrophic cyanobacteria, the signaling molecule bis-(3′-5′)-cyclic dimeric-guanosine monophosphate (c-di-GMP) plays important roles in a wide variety of functions associated with environmental conditions, including biofilm formation, motility ...
Chihiro C. Yamaguchi, Robert A. Kanaly, Eri Nishizaki, Keiichi Yamashita, Koichiro Takatsuki, Yoshihiko Furuike, Mingxu Fang, Shuji Akiyama, Setsuyuki Aoki, Masaki Tsukamoto, Yoichi Nakahira, Susan S. Golden, Shinsuke Kutsuna   +12 more
doaj   +1 more source

Cyclic GMP-AMP Synthase in Cancer Prevention. [PDF]

J Cancer Prev, 2023
Chen W, Lee GE, Jeung D, Byun J, Juan W, Cho YY.   +5 more
europepmc   +1 more source

Cell and Gene Therapies Manufacturing Challenges and Integrated Good Manufacturing Practices Solutions: A Lifecycle Perspective

Biotechnology and Bioengineering, EarlyView.
Lifecycle perspective on cell and gene therapy manufacturing challenges and enabling GMP solutions. ABSTRACT Cell and gene therapies (CGTs) are revolutionizing modern medicine; however, making these advanced medicines scalable and readily available to commercial manufacturers worldwide is a major challenge. The number of approved CGT products continues
Rajath Samaga, Udisha Singh, Sachin Dattram Pawar   +2 more
wiley   +1 more source

Identification of Putative Cellulose Synthase Gene xcsA2 Required for c‐di‐GMP‐Induced Biofilm Formation and Cell Aggregation in Synechococcus elongatus PCC 7942

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Synechococcus elongatus PCC 7942 is a widely used model cyanobacterium for diverse research fields and for bioproduction applications owing to its ease of cultivation, genetic modification, and ability to fix CO2. We found that a laboratory strain of S. elongatus PCC 7942 expressing the ydeH gene from E.
Chihiro C. Yamaguchi, Jiro F. Mori, Shinsuke Kutsuna, Robert A. Kanaly   +3 more
wiley   +1 more source

Efficient Cell Removal and Impurity Reduction for Poxvirus Purification in a Single Step Alluvial Filtration

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Modified Vaccinia virus Ankara (MVA) is a potent vector for the development of vaccines and novel cancer treatment strategies. Since it is a non‐replicating virus in human recipients, high infective titers are required for therapeutic efficacy.
Linus G. Weber, Caroline Stephan, Georgios Makris, Leander John, Ingo Jordan, Michael W. Wolff   +5 more
wiley   +1 more source

10‐Fold Improvement in Depth Filter Capacity During Integrated Dynamic Bioprocessing (IDB) Purification Through Depth Filter Cycling and Precipitate Control Strategy

Biotechnology and Bioengineering, EarlyView.
ABSTRACT During bsAb1 purification, precipitate was formed during neutralization of the affinity capture product. This precipitate was removed before loading onto the first polishing column in the Integrated Dynamic Bioprocessing (IDB) platform.
David Scherr, Ana Kanazir, Samik Das, Nikunj Sharda, Jan Martinek, David Nellis, Michael Mollet, Jon Coffman   +7 more
wiley   +1 more source