Results 61 to 70 of about 41,400 (261)

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Endothelial Plasticity: Shifting Phenotypes through Force Feedback

Stem Cells International, 2016
The endothelial lining of the vasculature is exposed to a large variety of biochemical and hemodynamic stimuli with different gradients throughout the vascular network.
Guido Krenning, Valerio G. Barauna, José E. Krieger, Martin C. Harmsen, Jan-Renier A. J. Moonen   +4 more
doaj   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Elasto-plasticity and pore-pressure coupled analysis on the pullout behaviors of a plate anchor

Theoretical and Applied Mechanics Letters, 2015
A numerical method is proposed for the elasto-plasticity and pore-pressure coupled analysis on the pullout behaviors of a plate anchor. The bounding-surface plasticity (BSP) model combined with Biot’s consolidation theory is employed to simulate the ...
Cun Hu, Fu-Ping Gao
doaj   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Cyclic Elasto-Plastic Behaviour of 51CrV4 Steel for Railway Parabolic Leaf Spring Design

Applied Sciences
Parabolic leaf springs are components typically found in suspensions of freight railway rolling stock. These components are produced in high-strength alloyed steel, DIN 51CrV4, to resist severe loading and environmental conditions. Despite the material’s
Vítor M. G. Gomes, Rita Dantas, José A. F. O. Correia, Abílio M. P. de Jesus   +3 more
doaj   +1 more source

Surface Morphology and Its Influence on Cyclic Deformation Behavior of High-Mn TWIP Steel

Metals, 2018
In this study, the dependence of the cyclic deformation behavior on the surface morphology of metastable austenitic HSD® 600 TWinning Induced Plasticity (TWIP) steel was investigated.
Matthias W. Klein, Marek Smaga, Tilmann Beck   +2 more
doaj   +1 more source

Strain Space Plasticity Model for Cyclic Mobility

Soils and Foundations, 1992
ABSTRACT The cyclic mobility model proposed in this paper is of a generalized plasticity—multiple mechanism type. The salient feature of the present approach is that the concept of the multiple mechanism, within the framework of plasticity theory defined in strain space, is used as a vehicle for decomposing the complex mechanism into a set of one ...
IAI, SUSUMU, MATSUNAGA, YASUO, KAMEOKA, TOMOHIRO   +2 more
openaire   +2 more sources

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source