Results 1 to 10 of about 1,033 (138)

Effect of cyclodextrin glucosyltransferase extracted from Bacillus xiaoxiensis on wheat dough and bread properties [PDF]

Frontiers in Nutrition, 2022
In this study, the cyclodextrin glucosyltransferase (CGTase) was extracted from Bacillus xiaoxiensis. CGTase had negative effects on dough viscoelastic properties and gluten strength but had positive effects on bread baking qualities and anti-staling ...
Lianzhan Yang, Jinxin Cai, Haifeng Qian, Yan Li, Hui Zhang, Xiguang Qi, Li Wang, Guoliang Cao   +7 more
doaj   +4 more sources

Effect of Leu²⁷⁷ on Disproportionation and Hydrolysis Activity in Bacillus stearothermophilus NO2 Cyclodextrin Glucosyltransferase. [PDF]

Appl Environ Microbiol, 2021
In this study, mutations that enhance the disproportionation to hydrolysis ratio of a CGTase have been discovered. For example, the disproportionation-to-hydrolysis ratio of the L277M mutant of Bacillus stearothermophilus NO2 CGTase was 2.4-fold greater than that of the wild type.
Kong D, Wang L, Su L, Wu J.
europepmc   +4 more sources

Preliminary Study of Reaction Conditions for the Synthesis of AA-2G by Cyclodextrin Glucosyltransferase

Shipin gongye ke-ji, 2023
To investigate the effect of cyclodextrin glucosyltransferase (CGTase) produced by the recombinant strain pET-28a(+)-cgt-T1/BL21 (DE3) on the synthesis of 2-O-α-D-glucoside (AA-2G), the recombinant protein CGTase-T1 was expressed in the fermentation ...
Yamei HE, Zhenyang LIU, Wan ZHENG, Ruiyang SU, Huawei WU   +4 more
doaj   +2 more sources

A two-stage temperature control strategy enhances extracellular secretion of recombinant α-cyclodextrin glucosyltransferase in Escherichia coli. [PDF]

AMB Express, 2017
The effects of temperature on extracellular secretion of the α-cyclodextrin glucosyltransferase (α-CGTase) from Paenibacillus macerans JFB05-01 by Escherichia coli were investigated. When protein expression was induced at constant temperature, the greatest amount of extracellular recombinant α-CGTase was produced at 25 °C.
Li Y, Liu J, Wang Y, Liu B, Xie X, Jia R, Li C, Li Z.   +7 more
europepmc   +4 more sources

Cyclodextrin glucosyltransferase production by Bacillus megaterium NCR: evaluation and optimization of culture conditions using factorial design. [PDF]

Indian J Microbiol, 2010
Statistically-based experimental designs were used to optimize the production of cyclodextrin glucosyltransferase (CGTase) from a local isolate of Bacillus megaterium using shack culture fermentation. Seven cultural conditions were examined for enzyme production and specific activity using Plackett-Burman factorial design. Fermentation time and K(2)HPO(
Ahmed EM, El-Refai HA.
europepmc   +4 more sources

Expression and Enzymatic Properties of Domain-Deleted Mutants of Cyclodextrin Glucosyltransferase my20 [PDF]

Shipin Kexue
In this study, we constructed domain D, E and DE truncated mutants of a marine-derived cyclodextrin glucosyltransferase (CGTase) my20 and heterologously expressed these mutants using the pET-24a vector and E. coli BL21 (DE3) as the host, and purified the
SUN Tengteng, WANG Wei, SUN Jingjing, JIANG Chengcheng, HAO Jianhua
doaj   +2 more sources

Nucleotide sequence of a cyclodextrin glucosyltransferase gene, cgtA, from Bacillus licheniformis. [PDF]

Nucleic Acids Res, 1990
Cyclodextrin glucosyltransferases (CGTases), EC 2.4.1.19, convert starch into cyclic glucosyl oligosaccharides (cyclodextrins) having a hydrophilic surface and a hydrophobic core (1). Thus, cyclodextrins bind to and solubilize hydrophobic materials. Recently, a potential therapeutic benefit for cyclodextrins was demonstrated (2).
Hill DE, Aldape R, Rozzell JD.
europepmc   +4 more sources

Glycosidic bond specificity of glucansucrases:on the role of acceptor substrate binding residues [PDF]

, 2012
Many lactic acid bacteria produce extracellular alpha-glucan polysaccharides using a glucansucrase and sucrose as glucose donor. The structure and the physicochemical properties of the alpha-glucans produced are determined by the nature of the ...
Dijkhuizen, Lubbert, Dijkstra, Bauke W., Dobruchowska, Justyna M., Leemhuis, Hans, Pijning, Tjaard   +4 more
core   +11 more sources

Research Progress on Synthesis and Application of Cyclodextrin Polymers

Molecules, 2021
Cyclodextrins (CDs) are a series of cyclic oligosaccharides formed by amylose under the action of CD glucosyltransferase that is produced by Bacillus. After being modified by polymerization, substitution and grafting, high molecular weight cyclodextrin ...
Yuan Liu, Ting Lin, Cui Cheng, Qiaowen Wang, Shujin Lin, Chun Liu, Xiao Han   +6 more
doaj   +1 more source

Heterologous Expression of Cyclodextrin Glycosyltransferase my20 in Escherichia coli and Its Application in 2-O-α-D-Glucopyranosyl-L-Ascorbic Acid Production

Frontiers in Microbiology, 2021
In this study, the cgt gene my20, which encodes cyclodextrin glycosyltransferase (CGTase) and was obtained by the metagenome sequencing of marine microorganisms from the Mariana Trench, was codon optimized and connected to pET-24a for heterologous ...
Kai Song, Jingjing Sun, Jingjing Sun, Jingjing Sun, Wei Wang, Wei Wang, Wei Wang, Jianhua Hao, Jianhua Hao, Jianhua Hao   +9 more
doaj   +1 more source