Results 131 to 140 of about 1,072 (175)
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The Chemical Engineering Journal and the Biochemical Engineering Journal, 1996
Abstract Production of cyclodextrin glucosyltransferase (CGTase) enzymes by six bacterial cultures was investigated. Enzyme production was better in shaken than in surface cultures. Bacillus macerans 314 and B, amyloliquifaciens cultured on potato dextrose (PD) medium were the most potent strains used.
Abdel-Mohsen S. Ismail +2 more
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Abstract Production of cyclodextrin glucosyltransferase (CGTase) enzymes by six bacterial cultures was investigated. Enzyme production was better in shaken than in surface cultures. Bacillus macerans 314 and B, amyloliquifaciens cultured on potato dextrose (PD) medium were the most potent strains used.
Abdel-Mohsen S. Ismail +2 more
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Biotechnology Techniques, 1995
A β-cyclodextrin glucosyltransferase was purified from alkalophilic Bacillus sp. No. 562 over 64-fold with a yield of 32%. Its molecular size was estimated to be 170 kDa by gel filtration and 82 kDa by SDS-PAGE, with a pI of 7.2. The enzyme showed optimum activity at 65 °C and pH 7.0. It was stable from 0 to70 °C and from pH 7.0 to 11.0. The enzyme was
Tsong-Rong Yan, Tsu-Tai Lin
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A β-cyclodextrin glucosyltransferase was purified from alkalophilic Bacillus sp. No. 562 over 64-fold with a yield of 32%. Its molecular size was estimated to be 170 kDa by gel filtration and 82 kDa by SDS-PAGE, with a pI of 7.2. The enzyme showed optimum activity at 65 °C and pH 7.0. It was stable from 0 to70 °C and from pH 7.0 to 11.0. The enzyme was
Tsong-Rong Yan, Tsu-Tai Lin
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[Engineering the 182 site of cyclodextrin glucosyltransferase for glycosylated genistein synthesis].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2022Genistein and its monoglucoside derivatives play important roles in food and pharmaceuticals fields, whereas their applications are limited by the low water solubility. Glycosylation is regarded as one of the effective approaches to improve water solubility.
Baocheng, Chai +3 more
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Biochemical Engineering Journal, 2019
Abstract An enzymatic approach to β-cyclodextrin synthesis attracts the attention of food engineers and chemists because of the broad applications of β-cyclodextrin in the food industry and the demand for a green process for β-cyclodextrin production.
Jianguo Zhang +3 more
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Abstract An enzymatic approach to β-cyclodextrin synthesis attracts the attention of food engineers and chemists because of the broad applications of β-cyclodextrin in the food industry and the demand for a green process for β-cyclodextrin production.
Jianguo Zhang +3 more
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Journal of the Chemical Society, Perkin Transactions 1, 1991
The synthesis of α-maltosyl fluorides substituted at the 6- or 6′-position with H, F, Br, OMe, OAc is described, together with the preparation of the 4-thio-α-maltosyl fluoride and 5-thin-α-D-glucosyl fluoride. These compounds were obtained by chemical or enzymatic procedures and their structures have been established by 13C NMR spectroscopy.
Cottaz, S., Apparu, C., Driguez, H.
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The synthesis of α-maltosyl fluorides substituted at the 6- or 6′-position with H, F, Br, OMe, OAc is described, together with the preparation of the 4-thio-α-maltosyl fluoride and 5-thin-α-D-glucosyl fluoride. These compounds were obtained by chemical or enzymatic procedures and their structures have been established by 13C NMR spectroscopy.
Cottaz, S., Apparu, C., Driguez, H.
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Carbohydrate Research, 1998
Radiolabeled linear and cyclic maltodextrins of high specific radioactivity were prepared using enzymes involved in maltodextrin metabolism. 14C-Labeled maltose was the starting material yielding products of identical specific radioactivity with respect to glucosyl residues. The enzymatic steps involved: i) Formation of linear 14C-labeled maltodexrins (
M, Pajatsch, A, Böck, W, Boos
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Radiolabeled linear and cyclic maltodextrins of high specific radioactivity were prepared using enzymes involved in maltodextrin metabolism. 14C-Labeled maltose was the starting material yielding products of identical specific radioactivity with respect to glucosyl residues. The enzymatic steps involved: i) Formation of linear 14C-labeled maltodexrins (
M, Pajatsch, A, Böck, W, Boos
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Process Biochemistry, 2010
Abstract The purpose of this study was to investigate the effect of medium additives on the secretion of recombinant α-cyclodextrin glucosyltransferase (α-CGTase) into the culture media of Escherichia coli. It is found that supplementation of the E.
Runrong Ding +5 more
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Abstract The purpose of this study was to investigate the effect of medium additives on the secretion of recombinant α-cyclodextrin glucosyltransferase (α-CGTase) into the culture media of Escherichia coli. It is found that supplementation of the E.
Runrong Ding +5 more
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Journal of Molecular Catalysis B: Enzymatic, 2015
Bacillus amyloliquefaciens cyclodextrin glucosyltransferase (CGTase) was immobilized on 35 supports by different methods of immobilization. The immobilized enzymes were prepared by physical adsorption on chitin, ionic binding onto Amberalite IRA-45, covalent binding on Duolite XAD761, and entrapment in polyacrylamide had the highest recovered activity.
Mohamed A. Abdel-Naby +2 more
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Bacillus amyloliquefaciens cyclodextrin glucosyltransferase (CGTase) was immobilized on 35 supports by different methods of immobilization. The immobilized enzymes were prepared by physical adsorption on chitin, ionic binding onto Amberalite IRA-45, covalent binding on Duolite XAD761, and entrapment in polyacrylamide had the highest recovered activity.
Mohamed A. Abdel-Naby +2 more
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Tetrahedron, 2004
Abstract Cyclodextrin glucosyltransferase (CGTase) from Thermoanaerobacter sp. was covalently immobilized on Eupergit C and used for the synthesis of maltooligosyl fructofuranosides employing soluble starch as donor and sucrose as acceptor. Using a weight ratio starch–sucrose of 1:2, the conversion of starch into acceptor products catalyzed by ...
M.Teresa Martı́n +5 more
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Abstract Cyclodextrin glucosyltransferase (CGTase) from Thermoanaerobacter sp. was covalently immobilized on Eupergit C and used for the synthesis of maltooligosyl fructofuranosides employing soluble starch as donor and sucrose as acceptor. Using a weight ratio starch–sucrose of 1:2, the conversion of starch into acceptor products catalyzed by ...
M.Teresa Martı́n +5 more
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Biochemical Engineering Journal, 2000
Abstract The immobilized cells of five bacterial cultures on different carriers were investigated for the production of cyclodextrin glucosyltransferase (CGTase). The entrapped cells of Bacillus amyloliquefaciens in calcium alginate showed the highest enzyme activity (70.8 U ml−1).
Mohamed A Abdel-Naby +2 more
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Abstract The immobilized cells of five bacterial cultures on different carriers were investigated for the production of cyclodextrin glucosyltransferase (CGTase). The entrapped cells of Bacillus amyloliquefaciens in calcium alginate showed the highest enzyme activity (70.8 U ml−1).
Mohamed A Abdel-Naby +2 more
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