Results 141 to 150 of about 1,072 (175)

Glucosylation of flavonol and flavanones by Bacillus cyclodextrin glucosyltransferase to enhance their solubility and stability

Food Chemistry, 2017
Enzymatically modified isoquercitrin (EMIQ), oligoglucosyl naringenin-7-(glucose [G]), and oligoglucosyl hesperetin (H)-7-G were produced via oligoglucosylation of quercetin-3-glucose, naringenin-7-G (prunin), and H-7-G, respectively, by cyclodextrin glucosyltransferase from Bacillus macerans.
Young-Su, Lee, Jang-Bin, Woo, Soo-In, Ryu, Seong-Kwon, Moon, Nam Soo, Han, Soo-Bok, Lee   +5 more
openaire   +2 more sources

Fast purification of cyclodextrin-glucosyltransferase fromBacillus circulans E 192 by affinity chromatography using an epichlorhydrin-reticulated copolymer of beta-cyclodextrin

Chromatographia, 1991
Bacillus circulans E 192 Cyclodextrin-glucosyltransferase was purified 40 fold to homogeneity on a large scale, by affinity chromatogrpahy on a β-CD copolymer cross-linked with epichlorhydrin. A preliminary step was necessary to remove maltooligosaccharides and cyclomaltooligosaccharides from the crude extract.
J. R. Villette, P. J. Looten, S. J. L. Bouquelet   +2 more
openaire   +1 more source

Structural characterization of slow digestion dextrin synthesized by a combination of α-glucosidase and cyclodextrin glucosyltransferase and its prebiotic potential on the gut microbiota in vitro

Food Chemistry, 2023
Starch-based dietary fibers are at the forefront of functional ingredient research. In this study, a novel water-soluble slow digestion dextrin (SDD) was synthesized by synergy of α-glucosidase and cyclodextrin glucosyltransferase and characterized. Results showed that SDD exhibited high solubility, low viscosity, and resistance to digestive enzymes ...
Beibei, Wei, Lei, Wang, Lingqia, Su, Xiumei, Tao, Sheng, Chen, Jing, Wu, Wei, Xia   +6 more
openaire   +2 more sources

Immobilization on Eupergit C of cyclodextrin glucosyltransferase (CGTase) and properties of the immobilized biocatalyst

Journal of Molecular Catalysis B: Enzymatic, 2003
Abstract The extreme thermophilic cyclodextrin glucanotransferase (CGTase) from Thermoanaerobacter sp. was covalently attached to Eupergit C. Different immobilization parameters (incubation time, ionic strength, pH, ratio enzyme/support, etc.) were optimized.
M.Teresa Martı́n, Francisco J Plou, Miguel Alcalde, Antonio Ballesteros   +3 more
openaire   +1 more source

Transfer reactions catalyzed by cyclodextrin glucosyltransferase using 4-thiomaltosyl and C-maltosyl fluorides as artificial donors

Carbohydrate Research, 1997
Cyclodextrin glycosyltransferase enzyme from Bacillus circulans catalyzed the effective conversion of 4-thio-alpha-maltosyl fluoride into cyclo-alpha-(1-->4(2))-thiomalto -tetraoside, -pentaoside, -hexaoside and linear hemithiomaltooligosaccharides.
Bornaghi, L., Utille, J.P., Rekai, E.D., Mallet, Jean-Maurice, Sinay, P., Driguez, H.   +5 more
openaire   +2 more sources

Site-saturation mutagenesis of proline 176 in Cyclodextrin Glucosyltransferase from Bacillus sp. Y112 effects product specificity and enzymatic properties

Process Biochemistry, 2020
Abstract Based on the analysis of amino acid sequence and simulated structure, saturation mutagenesis was performed to explore the role of the site p176 of cyclodextrin glucosytransferase (CGTase) from Bacillus sp. Y112. Compared to the wild-type, mutant P176G showed 10.4 % improvement in conversion from starch to cyclodextrins (CDs), whose β-CD ...
Xiaohan Li, Jingjing Sun, Wei Wang, Jiaomei Guo, Kai Song, Jianhua Hao   +5 more
openaire   +1 more source

Biosynthesis of cyclodextrin glucosyltransferase by the free and immobilized cells of Bacillus cereus NRC7 in batch and continuous cultures.

Journal of applied microbiology, 2012
The objective of this study was to enhance the production of cyclodextrin glucanotransferase (CGTase) produced by a local isolate Bacillus cereus NRC7.In batch culture, maximal CGTase activity (69·0 U ml(-1)) was reached after 24-h incubation period. In continuous production of CGTase by the free cells of B.
M A, Abdel-Naby, H A, El-Refai, A F, Abdel-Fattah   +2 more
openaire   +3 more sources

Use of Megaprimer and Overlapping Extension PCR (OE-PCR) to Mutagenize and Enhance Cyclodextrin Glucosyltransferase (CGTase) Function

2016
Protein engineering is a very useful tool for probing structure-function relationships in proteins. Specifically, site-directed mutagenized proteins can provide useful insights into structural, binding and catalytic mechanisms of a protein, particularly when coupled with crystallization.
Goh, Kian Mau, Liew, Kok Jun, Chai, Kian Piaw, Md. Illias, Rosli   +3 more
openaire   +3 more sources

Immobilization of Paenibacillus macerans NRRL B-3186 cyclodextrin glucosyltransferase and properties of the immobilized enzyme

Process Biochemistry, 1999
Abstract Cyclodextrin glucosyltransferase from Paenibacillus macerans NRRL B-3186 was immobilized on aminated polyvinylchloride (PVC) by covalent binding with a bifunctional agent (glutaraldehyde). The immobilized activity was affected by the length of the hydrocarbon chain attached to the PVC matrix, the amount of the protein loaded on the PVC ...
openaire   +1 more source

Biospecific sorption of cyclodextrin glucosyltransferase on physically modified starch

Journal of Chromatography B: Biomedical Sciences and Applications, 1988
M, Gottvaldova, H, Hrabova, V, Sillinger, J, Kucera   +3 more
openaire   +2 more sources