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Differentiation between Cyprinid herpesvirus type-3 lineages using duplex PCR

Journal of Virological Methods, 2009
To date, all the isolates of Cyprinid herpesvirus type-3 (CyHV3) responsible for serious outbreaks in carps Cyprinus carpio have been found to be very similar or identical on the basis of DNA sequences of a few reference genes. However, two genetic lineages (U/I and J) are distinguished by full-length genome sequencing. Two molecular markers presenting
Bigarre, L.   +8 more
openaire   +2 more sources

Nationwide Cyprinid herpesvirus 3 contamination in natural rivers of Japan

Research in Veterinary Science, 2012
Cyprinid herpesvirus 3 (CyHV-3) disease is a significant threat for common and koi carp cultivators and for freshwater ecosystems. To determine the prevalence of CyHV-3 in Japanese rivers, a nationwide survey of all national class-A rivers was undertaken in the Summer of 2008.
Toshifumi, Minamoto   +4 more
openaire   +2 more sources

Impact of cyprinid herpesvirus 3 (Koi herpesvirus) on wild and cultured fish.

CABI Reviews, 2016
Abstract Common carp is a widespread freshwater fish inhabiting lower and mid streams of rivers and shallow confined waters in Eurasia. Carp aquaculture in shallow, man-made ponds has a long history and now common carp is the most widely cultured freshwater fish species in the world.
D. Steinhagen   +2 more
openaire   +1 more source

Pathogenesis of acute and chronic diseases caused by cyprinid herpesvirus‐3

Journal of Fish Diseases, 2014
AbstractThe pathogenesis of cyprinid herpesvirus‐3 (CyHV‐3) was studied using different lineages of carp/koi. After exposure to the virus, infected cells were first found in the skin by histopathology and by in situ hybridization. The epidermis of the skin was most severely damaged and often sloughed off in the fish sampled on days 5 through 8, and the
S, Miwa   +4 more
openaire   +2 more sources

[Research Advances in Cyprinid Herpesvirus 3].

Bing du xue bao = Chinese journal of virology, 2016
Cyprinid herpesvirus 3 (CyHV-3) is the causative agent of an extremely contagious and aggressive disease afflicting common corp Cyprinus carpio L. termed koi herpesvirus disease (KHVD). Since it was first reported in 1997, the virus has spread worldwide rapidly, leading to enormous financial losses in industries based on common carp and koi carp.
Shucheng, Zheng   +7 more
openaire   +1 more source

Diagnostic validation of three test methods for detection of cyprinid herpesvirus 3 (CyHV-3)

Diseases of Aquatic Organisms, 2017
Cyprinid herpesvirus 3 (CyHV-3) is the aetiological agent of koi herpesvirus disease in koi and common carp. The disease is notifiable to the World Organisation for Animal Health. Three tests-quantitative polymerase chain reaction (qPCR), conventional PCR (cPCR) and virus isolation by cell culture (VI)-were validated to assess their fitness as ...
Sharon C, Clouthier   +9 more
openaire   +2 more sources

Biology and host response to Cyprinid herpesvirus 3 infection in common carp

Developmental & Comparative Immunology, 2014
Viruses from the family Alloherpesviridae form an aquatic clade of herpesviruses infecting fish and amphibia. Diseases caused by these herpesviruses are of increasing importance because of the high morbidity and mortality associated with the infection, and the difficulties in diagnosing latently infected carriers.
Mikołaj, Adamek   +5 more
openaire   +2 more sources

Transmission of Cyprinid herpesvirus-3 (CyHV-3) from goldfish to naïve common carp by cohabitation

Research in Veterinary Science, 2011
Cyprinid herpesvirus-3 (CyHV-3) has spread worldwide and has had a major impact on koi and common carp production. Previous studies on the host range of the CyHV-3 found that fish species other than koi and common carp are fully resistant to natural virus exposure.
Mansour, El-Matbouli, Hatem, Soliman
openaire   +2 more sources

Reservoirs of Cyprinid herpesvirus 3 (CyHV-3) DNA in sediments of natural lakes and ponds

Veterinary Microbiology, 2012
Cyprinid herpesvirus 3 (CyHV-3) is a lethal DNA virus that infects common carp and koi. It has caused outbreak of the disease within both aquaculture and natural environmental ecosystems. However, there is not enough understanding of the distribution of CyHV-3 in the natural environments, partly because there is no suitable quantification method.
Mie N, Honjo   +2 more
openaire   +2 more sources

Rapid detection of Cyprinid herpesvirus-3 (CyHV-3) using a gold nanoparticle-based hybridization assay

Journal of Virological Methods, 2015
Cyprinid herpesvirus-3 (CyHV-3) is a highly infectious pathogen that causes fatal disease in common and koi carp Cyprinus carpio L. CyHV-3 detection is usually based on virus propagation or amplification of the viral DNA using the PCR or LAMP techniques. However, due to the limited susceptibility of cells used for propagation, it is not always possible
Mona, Saleh, Mansour, El-Matbouli
openaire   +2 more sources

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