Results 31 to 40 of about 107 (57)

Isolation and characterization of a cDNA from flowers of Cynara cardunculus encoding cyprosin (an aspartic proteinase) and its use to study the organ-specific expression of cyprosin

open access: yesPlant Molecular Biology, 1994
Poly(A)+ RNA isolated from flower buds of Cynara cardunculus has been used to prepare a cDNA library. Screening of the cDNA after expression of cloned DNA with antibodies raised against the large subunit of cyprosin 3 resulted in the isolation of six positive clones. One of these clones (cypro1s; a 1.7 kb Eco RI fragment) codes for cyprosin.
Peter E Brodelius   +2 more
exaly   +13 more sources

Use of chemometrics in the selection of a Saccharomyces cerevisiae expression system for recombinant cyprosin B production [PDF]

open access: yesBiotechnology Letters, 2011
Two multivariate statistical methods, factor analysis (FA) and hierarchical cluster analysis (HCA), were applied to experimental data set to evaluate their usefulness in selecting the adequate expression system and optimal growth parameters for recombinant cyprosin B production. Using FA, the large data set was reduced to two factors representing 73.4%
Luís Fonseca   +2 more
exaly   +5 more sources

Comparative analysis of near and mid-infrared spectroscopy to monitor recombinant cyprosin production

open access: yes2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG), 2015
Infrared spectroscopy, either in the near and mid (NIR/MIR) region of the spectra, has gained great acceptance in the industry for bioprocess monitoring according to Process Analytical Technology, due to its rapid, economic, high sensitivity mode of application and versatility.
Cecília R. Calado, Pedro N. Sampaio
exaly   +4 more sources

Overexpression and characterization of cyprosin B in transformed suspension cells of Cynara cardunculus

open access: yesPlant Cell, Tissue and Organ Culture, 2010
Cynara cardunculus suspension cells were transformed by particle bombardment to overexpress the cypro11 gene coding for cyprosin B. Green fluorescent protein, used as a visual reporter through mgfp4-ER gene, facilitates the screening of transformed cells at the initial stages when antibiotics cause generalized cell death.
Susana Serrazina
exaly   +3 more sources

Plant Aspartic Proteinases from Cynara Cardunculus Spp. Flavescens Cv. Cardoon; Nucleotide Sequence of a cDNA Encoding Cyprosin and its Organ-Specific Expression

open access: yesAdvances in Experimental Medicine and Biology, 1995
Flowers of the plant Cynara cardunculus spp. flavescens cv. cardoon have been traditionally used since many centuries to produce ewe’s cheese, in Portugal and Spain. One of the first reports on this use by farmers was made by Brotero in 1804 [1]. Today, the Serpa and Serra cheeses are some examples of typical and highly appreciated products in Portugal.
Peter E Brodelius, M Pietrzak, M S Pais
exaly   +4 more sources

In situ near infrared spectroscopy monitoring of cyprosin production by recombinant Saccharomyces cerevisiae strains [PDF]

open access: yesJournal of Biotechnology, 2014
Near infrared (NIR) spectroscopy was used to in situ monitoring the cultivation of two recombinant Saccharomyces cerevisiae strains producing heterologous cyprosin B. NIR spectroscopy is a fast and non-destructive technique, that by being based on overtones and combinations of molecular vibrations requires chemometrics tools, such as partial least ...
Sampaio, Pedro   +4 more
openaire   +4 more sources

Tissue‐specific expression of multiple forms of cyprosin (aspartic proteinase) in flowers of Cynara cardunculus

open access: yesPhysiologia Plantarum, 1994
Three heterodimeric aspartic proteinases (cyprosin 1, 2 and 3) with milk‐clotting activity have previously been purified from flowers of Cynara cardunculua and partly characterized (U. Heimgartner et al. 1990, Phytochemistry 29: 1405–1410). These proteinases have now been further studied.
Maria C. Cordeiro   +2 more
core   +3 more sources

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