Results 41 to 50 of about 107 (57)
Some of the next articles are maybe not open access.

Use of Recombinant Cyprosin in the Manufacture of Ewe's Milk Cheese

Journal of Agricultural and Food Chemistry, 2003
A recombinant cyprosin from the cardoon (Cynara cardunculus L.) was assayed and compared with calf rennet in batches of ewes' milk cheese by determining different chemical, biochemical, and microbiological parameters over 4 months of ripening. There were no differences between the two types of coagulants in most chemical parameters, a(w), and pH ...
Luis Tejada   +2 more
exaly   +3 more sources

A novel fed-batch based strategy for enhancing cell-density and recombinant cyprosin B production in bioreactors

Bioprocess and Biosystems Engineering, 2014
Nowadays, the dairy industry is continuously looking for new and more efficient clotting enzymes to create innovative products. Cyprosin B is a plant aspartic protease characterized by clotting activity that was previously cloned in Saccharomyces cerevisiae BJ1991 strain.
Luís Fonseca   +2 more
exaly   +3 more sources

Microencapsulation of cyprosins from flowers ofCynara cardunculus L. in dehydration-rehydration liposomes

Biotechnology Letters, 1994
Cyprosin extract from dried flowers ofCynara cardunculus L. was dissolved in two different buffers (50 mM Tris-HCl, pH 8.3 and 10 mM PBS, pH 7.4), mixed with a sonicated soybean lecithin dispersion and microencapsulated in dehydration-rehydration liposomes.
Manuel Nuñez   +2 more
exaly   +2 more sources

Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. [PDF]

open access: yesFEBS Journal, 2007
Cardosin A and cardosin B are two aspartic proteases mainly found in the pistils of cardoon Cynara cardunculus L., whose flowers are traditionally used in several Mediterranean countries in the manufacture of ewe's cheese.
Carlos Faro   +2 more
exaly   +2 more sources

Production and characterization of recombinant cyprosin B in Saccharomyces cerevisiae (W303-1A) strain

Journal of Bioscience and Bioengineering, 2008
The Saccharomyces cerevisiae W303-1A strain transformed with a centromeric plasmid containing CYPRO11, which codifies the aspartic protease cyprosin B, was grown in a 3 l bioreactor under aerobic conditions. Expression of cyprosin B is directly dependent on the concentration of galactose used as the inducer and carbon source in 1% yeast extract, 2 ...
Pedro N, Sampaio   +4 more
openaire   +2 more sources

Microencapsulation of cyprosins from flowers of Cynara cardunculus L. in dehydration-rehydration liposomes

2023
Cyprosin extract from dried flowers of Cynara cardunculus L. was dissolved in two different buffers (50 mM Tris-HCl, pH 8.3 and 10 mM PBS, pH 7.4), mixed with a sonicated soybean lecithin dispersion and microencapsulated in dehydration-rehydration liposomes.
Picón Gálvez, Antonia María   +3 more
openaire   +1 more source

Aspartic Proteinases (Cyprosins) from Cynara Cardunculus Spp. Flavescens Cv. Cardoon; Purification, Characterisation, and Tissue-Specific Expression

1995
Flowers of C cardunculus (cardoon) have been used in Portugal since many centuries to make cheese with raw ewe or/and goat milk. Several types of cheeses are well known and highly appreciated for their taste and quality. Examples are Serra, Serpa and Azeitao.
P E, Brodelius, M C, Cordeiro, M S, Pais
openaire   +2 more sources

Substrate Specificity and Molecular Modelling of Aspartic Proteinases (Cyprosins) from Flowers of Cynara cardunculus SUBSP. flavescens CV. Cardoon

1998
Water extracts of dried flowers of Cynara cardunculus subsp. flavescens cv. cardoon have been traditionally used in Portugal to produce artisanal cheeses (e.g. Serpa and Serra cheeses) with ewe’s milk. The clotting activity is due to three heterodimeric aspartic proteinases (cyprosin 1, cyprosin 2 and cyprosin 3) which have been purified from flowers.1
M, Cordeiro   +6 more
openaire   +2 more sources

Cynara cardunculus subsp. flavescens (Cardoon): In Vitro Culture, and the Production of Cyprosins —Milk-Clotting Enzymes

1998
Flowers of Cynara cardunculus flavescens (cardoon) have been traditionally used in Portugal and Spain for many centuries to produce artisanal cheeses. One of the first reports on this use by farmers was made by Brotero (1804) in Flora Lusitanica. Today, the Serpa and Serra cheeses are some examples of typical and highly appreciated products of Portugal.
M. C. Cordeiro   +2 more
openaire   +1 more source

Home - About - Disclaimer - Privacy