Results 111 to 120 of about 277 (144)
Effects of mechanical wounding on Carica papaya cysteine endopeptidases accumulation and activity
The mechanical wounding impact on the Carica papaya latex protein pattern was investigated by analyzing three latexes. A first one commercially available, a second harvested from unripe but fully grown fruits, both obtained from regularly tapped fruits. A third one was collected from similar fruits but wounded for the first time.
Mohamed Azarkan+3 more
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Comparison of Two Cysteine Endopeptidases from Pseudananas macrodontes (Morr.) Harms (Bromeliaceae)
The properties of two cysteine peptidases (macrodontain I and II) isolated from fruits of Pseudananas macrodontes have been compared. The enzymes showed optimum pH ranges near neutrality and were inhibited by E-64 and other cysteine peptidase inhibitors.
Laura López+5 more
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Maturation of barley cysteine endopeptidase B (EPB) in Trichoderma reesei was studied with metabolic inhibitors, Western blotting, and immuno microscopy. The inactive 42-kDa recombinant EPB proprotein, first detected in apical cells, was sequentially processed in a time-dependent manner to a secreted polypeptide of 38.5 kDa, and thereafter, to ...
Marko Nykänen+3 more
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Role of cysteine endopeptidases (EC 3.4.22) in rabbit meat tenderisation and some related changes
The role of endogenous cysteine endopeptidases (EC 3.4.22; calpains and cathepsins B, L and H) in rabbit (Oryctolagus cuniculus L.) meat tenderisation and some related changes (formation of the 30 kDa fragment and myofibrillar structure weakening), during ageing (9 days at +4°C), was studied for different types of muscle (I, IIB and IID).
José A. M. Prates+2 more
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Role of Cysteine Endopeptidases in Cancerogenesis
Cancer Biotherapy and Radiopharmaceuticals, 1996Role of cysteine endopeptidases in cancerogenesis steps: neoplastic transformation, invasion and metastasis is reviewed and discussed. Positive correlation between tumor invasiveness, as well as its metastatic potential and secretion of cysteine endopeptidase (particularly cathepsins B and L) has been documented well in literature.
Andrzej Zarzycki+3 more
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[38] Pineapple cysteine endopeptidases
1994Publisher Summary This chapter focuses on pineapple cysteine endopeptidases. The pineapple plant contains at least four distinct cysteine endopeptidases. The major endopeptidase present in extracts of plant stem is stem bromelain, and fruit bromelain is the major endopeptidase in the fruit. Two additional cysteine endopeptidases, ananain and comosain,
Andrew D Rowan, David J. Buttle
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Biochemistry, 1995
Glycyl endopeptidase is a cysteine endopeptidase of the papain family, characterized by specificity for cleavage C-terminal to glycyl residues only and by resistance to inhibition by members of the cystatin family of cysteine proteinase inhibitors. Glycyl endopeptidase has been crystallized from high salt with a substrate-like inhibitor covalently ...
O'Hara, Bernard P.+3 more
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Glycyl endopeptidase is a cysteine endopeptidase of the papain family, characterized by specificity for cleavage C-terminal to glycyl residues only and by resistance to inhibition by members of the cystatin family of cysteine proteinase inhibitors. Glycyl endopeptidase has been crystallized from high salt with a substrate-like inhibitor covalently ...
O'Hara, Bernard P.+3 more
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Chinese hamster ovary cells continuously secrete a cysteine endopeptidase
In Vitro Cellular & Developmental Biology, 1990The protease activity in serum-free conditioned medium of chinese hamster ovary (CHO) cells was measured using peptidyl (or aminoacyl)-4-methylcoumaryl-7-amides (MCAs) as the substrates. Aminopeptidase increased in level as amounts of nonviable cells increased during cultivation in serum-free medium, indicating that the activity seems to be originated ...
Mitsuo Satoh, Shinji Hosoi, Seiji Sato
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[35] Cysteine endopeptidases of Entamoeba histolytica
1994Publisher Summary This chapter presents cysteine endopeptidases of Entamoeba histolytica. Entamoeba histolytica is a parasitic protozoon residing in the human gut. It is the etiological trigger of amebiasis, which is a widespread disease in tropical countries.
E. Tannich, Henning Scholze
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Toward Computer-Based Cleavage Site Prediction of Cysteine Endopeptidases
Biological Chemistry, 2003Identification of relevant substrates is essential for elucidation of in vivo functions of peptidases. The recent availability of the complete genome sequences of many eukaryotic organisms holds the promise of identifying specific peptidase substrates by systematic proteome analyses in combination with computer-based screening of genome databases ...
Lohmüller, T.+6 more
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