Results 91 to 100 of about 56,630 (276)

Single‐Cell Transcriptomic Profiling Identifies B Cell‐Intrinsic Dysregulation of Sphingolipid Biosynthesis and Could Be Improved by Inhibiting UGCG in Primary Immune Thrombocytopenia

Advanced Science, EarlyView.
This study generates the single‐cell atlas of pathogenic B‐cell subset Bc‐5 in ITP. It identifies RUNX3 as a key survival regulator and uncovers sphingolipid‐driven metabolic reprogramming, validating UGCG inhibition as a therapeutic candidate and building a valuable resource for ITP mechanistic and therapeutic exploration.
Dongmei Luo, Yanxia Zhan, Lili Ji, Pengcheng Xu, Pu Chen, Hao Pang, Yunan Ling, Fanli Hua, Xibing Zhuang, Hao Chen, Yunfeng Cheng   +10 more
wiley   +1 more source

Recent Advances in Virus–Host Interactions, Antiviral Bioactive Compounds, and Breeding for Disease Resistance of Porcine Epidemic Diarrhea Virus

Animal Research and One Health, EarlyView.
Basic research on the PEDV infection cycle and virus–host interactions advances the development of anti‐PEDV drugs and disease‐resistant breeding and helps strengthen disease prevention and control while reducing economic losses in the swine industry.
Heyong Wu, Aosi Qi, Shuyu Zhong, Haozhen Du, Shimeng Guo, Hongsheng Ouyang, Hongming Yuan, Daxin Pang, Zicong Xie   +8 more
wiley   +1 more source

Structural Basis for the Specificity, Catalysis, and Regulation of Human Uridine-Cytidine Kinase [PDF]

, 2004
Uridine-cytidine kinase (UCK) catalyzes the phosphorylation of uridine and cytidine and activates pharmacological ribonucleoside analogs. Here we present the crystal structures of human UCK alone and in complexes with a substrate, cytidine, a feedback ...
Fukushima, Masanori, Koizumi, Katsuhisa, Suzuki, Nobuo N, Matsuda, Akira, Inagaki, Fuyuhiko   +4 more
core   +1 more source

MiniSdd7‐Derived Cytosine Base Editor Promotes Efficient Multiplex Gene Knockout in Porcine Primary Fibroblasts

Animal Research and One Health, EarlyView.
The miniSdd7‐BE4max‐SpG system enables highly efficient multiplex C‐to‐T base editing of APN, CD163, and MSTN in porcine fibroblasts, providing a robust platform for future development of multiplex gene‐edited livestock. ABSTRACT Efficient multiplex genome editing is essential for improving complex traits in livestock that are governed by multiple ...
Shaoshuai Wang, Yu Wang, Dengfeng Bi, Jianguo Zhao, Yanfang Wang   +4 more
wiley   +1 more source

Early relapse on adjuvant gemcitabine associated with an exceptional response to 2nd line capecitabine chemotherapy in a patient with pancreatic adenosquamous carcinoma with strong intra-tumoural expression of cytidine deaminase: a case report

BMC Cancer, 2020
Background Pancreatic adenosquamous carcinoma has a poor prognosis, with limited prospective trial data to guide optimal treatment. The potential impact of drug metabolism on the treatment response of patients with pancreatic adenosquamous carcinoma is ...
Claire M. Connell, Rebecca Brais, Hayley Whitaker, Sara Upponi, Ian Beh, Jane Risdall, Pippa Corrie, Tobias Janowitz, Duncan I. Jodrell   +8 more
doaj   +1 more source

Uridine-cytidine kinase 2 promotes metastasis of hepatocellular carcinoma cells via the Stat3 pathway

, 2018
Qiming Zhou,1,2,* Hai Jiang,1–3,* Jianlong Zhang,1,2,* Wei Yu,1,2 Zhenyu Zhou,1,2 Pinbo Huang,1,2 Jie Wang,1,2 Zhiyu Xiao1,2 1Guangdong Province Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun ...
Zhou Q, Zhang J, Xiao Z, Jiang H, Yu W, Wang J, Zhou Z, Huang P   +7 more
core  

Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing

Animal Research and One Health, EarlyView.
Multiplex genome editing is transforming genome engineering from single‐gene perturbation to network‐level control, yet its broader application remains limited by challenges in gRNA array engineering, delivery technologies, and safety management. Emerging AI‐driven approaches are accelerating guide RNA design and CRISPR effector optimization for ...
Linli Wang, Yongbin Liu, Hongbing Han
wiley   +1 more source

Phosphonomethyl (Thio)Phosphonamidite Reagents for the Construction of Phosphonylmethyl Phosphono(Di)Thioate Mono‐ and Diesters

Chemistry – A European Journal, EarlyView.
A set of new phosphonomethyl (thio)phosphonamidite reagents is introduced to generate unprecedented pyrophosphate mimetics. The versatile P(III)‐P(V) reagents allow for the construction of various pyrophosphate isosteres, and the applicability of the chemistry is illustrated in the assembly of a diverse set of stabilized CDP‐glycerol and CDP‐ribitol ...
Pascal Balić, Roy Steneker, Gijsbert A. van der Marel, Jeroen D. C. Codée, Dmitri V. Filippov   +4 more
wiley   +1 more source

A Quantum Chemical Study of the Catalysis for Cytidine Deaminase:  Contribution of the Extra Water Molecule

, 2016
Cytidine deaminase is known as an important enzyme responsible for the hydrolytic deamination of cytidine, which is applied as a key step to the conversion of the precursor of the cancer drug to an active form in the living body.
Masashi Ishikura (2639875), Misako Aida (685555), Toshiaki Matsubara (1745320)   +2 more
core   +1 more source

Uncatalyzed Guanosine Phosphorylation under Potentially Prebiotic Conditions

Chemistry – A European Journal, EarlyView.
Pyrophosphate acts as phosphorylating agent of guanosine under simple wet‐dry cycles without condensing agent and inorganic catalysts to afford cyclic guanosine monophosphate derivatives useful for oligomerization in prebiotic processes. ABSTRACT The phosphorylation of nucleosides and their subsequent polymerization are key issues in the study of the ...
Martina Proietti, Bruno Mattia Bizzarri, Elisa De Marchi, Alessandra Giorgi, Raffaele Saladino, Giovanna Costanzo   +5 more
wiley   +1 more source