Results 131 to 140 of about 1,917 (174)

Human Enterovirus D68 infection - the intricate dance of cells, genes, and invading bugs. [PDF]

Front Cell Infect Microbiol
Lillerovde Ørstenvik H, Tveten AK, Cao Y.   +2 more
europepmc   +1 more source

Structural characterization and protective effects of polysaccharides isolated from <i>Berberis dasystachya</i> M. fruits on H₂O₂-induced oxidative damage in RINm5F pancreatic β-cells. [PDF]

Front Nutr
Lu X, Han L, Deng Y.
europepmc   +1 more source

Discrimination of superficial lymph nodes using ultrasonography and tissue metabolomics coupled with machine learning. [PDF]

Front Oncol
Li L, Wang X, Deng H, Lu W, Zhou Y, Ye X, Li Y, Wang J.   +7 more
europepmc   +1 more source

Variability of Sialic Acids in Beef Breeds and Nutritional Implications in Red Meat. [PDF]

Molecules
Contò M, Miarelli M, Di Giovanni S, Failla S.   +3 more
europepmc   +1 more source

Genetic engineering drives the breakthrough of pig models in liver disease research. [PDF]

Liver Res
Xu C, Fang X, Xu X, Wei X.
europepmc   +1 more source

On the topical issue of assisted dying: the insurmountable challenges of human existence, and the right to exit. [PDF]

Front Public Health
Tobore TO.
europepmc   +1 more source

Knockout of cytidine monophosphate-N-acetylneuraminic acid hydroxylase in Chinese hamster ovary cells by CRISPR/Cas9-based gene-editing technology

Biochemical Engineering Journal, 2020
Abstract Chinese hamster ovary (CHO) cells are mainly used mammalian host to produce therapeutic recombinant glycoproteins which are close to the human-like glycosylation of glycoproteins. However, cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH) in CHO cells can produce N-glycolylneuraminic acids (Neu5Gc) which elicits the immune ...
Yurong Chai, Xiang-Xiang Cao, Mengmeng Ge, Chunliu Mi, Xiao Guo, Tianyun Wang   +5 more
openalex   +2 more sources

Cloning and overexpression of a cytidine 5′-monophosphate N-acetylneuraminic acid synthetase from Escherichia coli

Journal of Molecular Catalysis B: Enzymatic, 2000
The gene for cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuAc) synthetase (EC2.7.7.43) was amplified from the total DNA of Escherichia coli 44277 through a primer-directed polymerase chain reaction and cloned into pUC19 between the sites EcoRI and SalI.
Guoqing Xia, Xing Han, Huiping Ding, Shoujun Yang, Shuzheng Zhang, Cheng Jin   +5 more
openalex   +3 more sources

Characterization of a Bifunctional Cytidine 5′-Monophosphate N-acetylneuraminic acid Synthetase Cloned from Streptococcus Agalactiae

Biotechnology Letters, 2005
Recombinant CMP-sialic acid synthetase, cloned from Streptococcus agalactiae serotype V strain 2603 V/R, is bifunctional having both CMP-sialic acid synthetase and acetylhydrolase (acylesterase) activities. The enzyme is active over a wide pH range with an optimal CMP-sialic acid synthetase activity at pH 9.0 and an optimal acetylhydrolase activity at ...
Hui Yu, Wesley Ryan, Hai Yu, Xi Chen
openalex   +3 more sources

Identification, expression and tissue distribution of cytidine 5′-monophosphate N-acetylneuraminic acid synthetase activity in the rat

Glycoconjugate Journal, 1998
We report the postnatal developmental profiles of N-acetylneuraminic acid cytidylyltransferase (EC 2.7.7.43) (CMP-Neu5Ac synthetase) in different rat tissues. This enzyme, which catalyses the activation of NeuAc to CMP-Neu5Ac, was detected in brain, kidney, heart, spleen, liver, stomach, intestine, lung, thymus, prostate and urinary bladder but not in ...
Beatriz Revilla‐Nuin, Ángel Reglero, John Feo, Leandro B. Rodrı́guez-Aparicio, Miguel Ángel Martín Ferrero   +4 more
openalex   +3 more sources