Results 1 to 10 of about 80,258 (202)

ADP-Ribosylation of Cytidine: A Novel Nucleic Acid Modification Reversed by NADAR Hydrolases [PDF]

Toxins
ADP-ribosylation of nucleic acids is a modification found in both eukaryotes and bacteria, where it contributes to genome maintenance but can also serve as a toxic mechanism used by bacterial toxins to disrupt essential cellular processes.
Petra Mikolčević, Andrea Hloušek-Kasun, Marion Schuller, Yang Lu, Elena Pirović, Ivan Ahel, Andreja Mikoč   +6 more
doaj   +2 more sources

Integrated amplification of NADPH-regenerating modules enhances cytidine biosynthesis in Escherichia coli [PDF]

Synthetic and Systems Biotechnology
In Escherichia coli, cofactor imbalance serves as a crucial limiting factor in cytidine biosynthesis, with nicotinamide adenine dinucleotide phosphate (NADPH) insufficiency representing the principal metabolic barrier.
Lu Liu, Xiangjun Zhang, Tengteng Zhu, Tong Ye, Zongqian Li, Wei Ding, Huiyan Liu, Haitian Fang   +7 more
doaj   +2 more sources

Indispensable residue for uridine binding in the uridine-cytidine kinase family

Biochemistry and Biophysics Reports, 2017
Uridine-cytidine kinase (UCK), including human UCK2, are a family of enzymes that generally phosphorylate both uridine and cytidine. However, UCK of Thermus thermophilus HB8 (ttCK) phosphorylates only cytidine.
Fumiaki Tomoike, Kenji Fukui, Takato Yano   +2 more
exaly   +3 more sources

Promoting cytidine biosynthesis by modulating pyrimidine metabolism and carbon metabolic regulatory networks in Bacillus subtilis [PDF]

Microbial Cell Factories
Background The modification of single or multiple genes via metabolic engineering can lead to the dysregulation of central metabolism and affect bacterial growth and metabolite accumulation.
Xiangjun Zhang, Lu Liu, Pilian Niu, Tong Ye, Wei Ding, Xiaobo Wei, Junnan Xu, Haitian Fang, Huiyan Liu   +8 more
doaj   +2 more sources

Double-strand break-free and transgene-free genome editing in the microalga Nannochloropsis oceanica using removable vectors containing the CRISPR base editing system [PDF]

Scientific Reports
The accumulation of lipids by algae makes them attractive for carbon-neutral fuel production; however, the industrial-scale production of algal lipids has yet to be achieved.
Keishi Moroi, Takashi Yamamoto, Tomokazu Kurita   +2 more
doaj   +2 more sources

Transdifferentiation of α-1,3-galactosyltransferase knockout pig bone marrow derived mesenchymal stem cells into pancreatic -like cells by microenvironment modulation [PDF]

Asian-Australasian Journal of Animal Sciences, 2020
Objective To evaluate the pancreatic differentiation potential of α-1,3-galactosyltransferase knockout (GalTKO) pig-derived bone marrow-derived mesenchymal stem cells (BM-MSCs) using epigenetic modifiers with different pancreatic induction media. Methods
Imran Ullah, Ran Lee, Keon Bong Oh, Seongsoo Hwang, Youngim Kim, Tai-Young Hur, Sun A Ock   +6 more
doaj   +1 more source

Microwave-Assisted Synthesis of 5′-O-methacryloylcytidine Using the Immobilized Lipase Novozym 435

Molecules, 2022
Nucleobase building blocks have been demonstrated to be strong candidates when it comes to DNA/RNA-like materials by benefiting from hydrogen bond interactions as physical properties. Modifying at the 5′ position is the simplest way to develop nucleobase-
Sany Chea, Khac Toan Nguyen, Ruben R. Rosencrantz   +2 more
doaj   +1 more source

The Effect of E. coli Uridine-Cytidine Kinase Gene Deletion on Cytidine Synthesis and Transcriptome Analysis

Fermentation, 2022
Cytidine is an antiviral and anticancer drug intermediate, its primary method of manufacture being fermentation. Uridine-cytidine kinase (UCK) catalyzes the reverse process of phosphorylation of cytidine to produce cytidylic acid, which influences ...
Fengmin Liu, Tong Ye, Xiangjun Zhang, Cong Ma, Huiyan Liu, Haitian Fang   +5 more
doaj   +1 more source

Efficient deamination of 5-methylcytidine and 5-substituted cytidine residues in DNA by human APOBEC3A cytidine deaminase. [PDF]

PLoS ONE, 2013
Deamination of 5-methylcytidine (5MeC) in DNA results in a G:T mismatch unlike cytidine (C) deamination which gives rise to a G:U pair. Deamination of C was generally considered to arise spontaneously.
Rodolphe Suspène, Marie-Ming Aynaud, Jean-Pierre Vartanian, Simon Wain-Hobson   +3 more
doaj   +1 more source

Bacillus anthracis edema factor substrate specificity: evidence for new modes of action [PDF]

, 2012
Since the isolation of Bacillus anthracis exotoxins in the 1960s, the detrimental activity of edema factor (EF) was considered as adenylyl cyclase activity only.
Agrawal, Altaweel, Basler, Bauche, Bell, Benson, Beste, Bock, Bond, Boyd, Bradley, Brown, Burhenne, Carbonetti, Carbonetti, Carson-Walter, Casteel, Christensen, Christensen, Confer, Copsel, Crawford, Crowe, Cunningham, Dalkas, Defer, Deuquet, Ding, Drum, Duesbery, Feld, Firoved, Friedlander, Fu, Fukuhara, Gaion, Gille, Gordon, Göttle, Hammerschmidt, He, Helfman, Hong, Ivarsson, Jernigan, Johnson, Katayama, Katayama, Kaupp, Kintzer, Klimpel, Laine, Larabee, Larabee, Leppla, Liu, Martin Göttle, McDonough, Milne, Mogridge, Mogridge, Newton, Newton, Newton, Newton, Newton, Oncu, O´Brien, Park, Petosa, Pezard, Postea, Puhar, Roland Seifert, Santelli, Scobie, Scobie, Scorpio, Shapiro, Shen, Shirshev, Soelaiman, Stefan Dove, Sun, Tamayo, Taylor, Taylor, Tesmer, Thomas, Tournier, Vitale, Vitale, Vojtova, Yeager, Zong   +94 more
core   +2 more sources