Results 21 to 30 of about 56,630 (276)

Integrated amplification of NADPH-regenerating modules enhances cytidine biosynthesis in Escherichia coli [PDF]

Synthetic and Systems Biotechnology
In Escherichia coli, cofactor imbalance serves as a crucial limiting factor in cytidine biosynthesis, with nicotinamide adenine dinucleotide phosphate (NADPH) insufficiency representing the principal metabolic barrier.
Lu Liu, Xiangjun Zhang, Tengteng Zhu, Tong Ye, Zongqian Li, Wei Ding, Huiyan Liu, Haitian Fang   +7 more
doaj   +2 more sources

Transdifferentiation of α-1,3-galactosyltransferase knockout pig bone marrow derived mesenchymal stem cells into pancreatic -like cells by microenvironment modulation [PDF]

Asian-Australasian Journal of Animal Sciences, 2020
Objective To evaluate the pancreatic differentiation potential of α-1,3-galactosyltransferase knockout (GalTKO) pig-derived bone marrow-derived mesenchymal stem cells (BM-MSCs) using epigenetic modifiers with different pancreatic induction media. Methods
Imran Ullah, Ran Lee, Keon Bong Oh, Seongsoo Hwang, Youngim Kim, Tai-Young Hur, Sun A Ock   +6 more
doaj   +1 more source

Microwave-Assisted Synthesis of 5′-O-methacryloylcytidine Using the Immobilized Lipase Novozym 435

Molecules, 2022
Nucleobase building blocks have been demonstrated to be strong candidates when it comes to DNA/RNA-like materials by benefiting from hydrogen bond interactions as physical properties. Modifying at the 5′ position is the simplest way to develop nucleobase-
Sany Chea, Khac Toan Nguyen, Ruben R. Rosencrantz   +2 more
doaj   +1 more source

Engineering a Cytidine Aminotransferase for Biocatalytic Production of the Antiviral Molnupiravir [PDF]

, 2021
The COVID-19 pandemic highlights the urgent need for cost-effective processes to rapidly manufacture antiviral drugs at scale. Here we report a concise biocatalytic process for Molnupiravir, a nucleoside analogue currently in phase 3 clinical trials as ...
Carl, Young, Ian, Rowles, Rebecca, Crawshaw, Sarah, Lovelock, Anthony, Green, Ashleigh, Burke, Bruna Zuculoto da, Costa, Nicholas, Turner, William, Birmingham, James, Finnigan, Ying, Zhuo, Simon J., Charnock, Thomas, Thorpe   +12 more
core   +1 more source

Photochemical Stability of 5-Methylcytidine Relative to Cytidine: Photophysical Insight for mRNA Therapeutic Applications [PDF]

, 2023
5-Methylcytidine (5mCyd) has recently been investigated with renewed interest for utilization in mRNA therapeutics. However, its photostability following exposure to electromagnetic radiation has been overlooked.
Sarah E., Krul, Erqian, Mao, Sean J., Hoehn, Michael M., Pogharian, Carlos, Crespo-Hernández   +4 more
core   +1 more source

Ribonucleotides Amplify Mitochondrial DNA-Driven cGAS-STING Activation via FdUMP-ribonucleotide Hybrid Particles to Potentiate Chemoimmunotherapy. [PDF]

Adv Sci (Weinh)
This work develops a metabolic sharing strategy that employs cytidine monophosphate (CMP) to competitively reprogram floxuridine (FUDR) pyrimidine metabolism in colorectal tumor cells. Elevated intracellular FdUMP induces nucleotide imbalance, drives mitochondrial DNA release and cGAS‐STING innate immune cascade activation, and potentiates robust ...
Wang C, Zhao C, Yao Y, Zou Z, Shi A, Feng Z, Yang M, Yu Y, Liu Y, Rui X, Wu J.   +10 more
europepmc   +2 more sources

A Concise Route to MK-4482 (EIDD-2801) from Cytidine: Part 2 [PDF]

, 2020
A new route to MK-4482 (1) was developed. The route replaces uridine with the more available and less expensive cytidine. Low cost, simple reagents are used for the chemical transformations and the yield is improved from 17% to 44%.
Timothy F., Jamison, Corshai, Williams, Vijayagopal, Gopalsamuthiram, Jeffrey, Noble, B. Frank, Gupton, David, Snead   +5 more
core   +2 more sources

The essential roles of cytidine diphosphate-diacylglycerol synthase in bloodstream form Trypanosoma brucei [PDF]

, 2014
Funded by Wellcome Trust: Senior Research Fellowship, Grant Number: 067441 and Wellcome Trust, Grant Numbers: 082596, 093228.Lipid metabolism in Trypanosoma brucei, the causative agent of African sleeping sickness, differs from its human host in several ...
Lilley, Alison C., Smith, Terry K., Smith, Terry K, Young, Simon, Stark, Michael J. R., Lilley, Alison Catherine, Major, Louise Laura, Major, Louise   +7 more
core   +1 more source

APOBEC3B drives PKR-mediated translation shutdown and protects stress granules in response to viral infection

Nature Communications, 2023
APOBEC’s are a family of cytidine deaminases that induce mutations in viruses to inhibit their replication and maintain cell integrity. Here, Manjunath et al show that APOBEC3B also inhibits viral replication by stimulating the innate immune sensor ...
Lavanya Manjunath, Sunwoo Oh, Pedro Ortega, Alexis Bouin, Elodie Bournique, Ambrocio Sanchez, Pia Møller Martensen, Ashley A. Auerbach, Jordan T. Becker, Marcus Seldin, Reuben S. Harris, Bert L. Semler, Rémi Buisson   +12 more
doaj   +1 more source

Highly Efficient CRISPR-Mediated Base Editing in Sinorhizobium meliloti

Frontiers in Microbiology, 2021
Rhizobia are widespread gram-negative soil bacteria and indispensable symbiotic partners of leguminous plants that facilitate the most highly efficient biological nitrogen fixation in nature.
Longxiang Wang, Yuan Xiao, Xiaowei Wei, Jimin Pan, Jimin Pan, Deqiang Duanmu, Deqiang Duanmu   +6 more
doaj   +1 more source