Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection [PDF]
Veterinary World, 2022 Background and Aim: We previously developed the gene-editing by electroporation (EP) of Cas9 protein method, in which the CRISPR/Cas9 system was introduced into porcine in vitro fertilized (IVF) zygotes through EP to disrupt a target gene.
Zhao Namula +8 more
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Enhanced Bacterial α(2,6)-Sialyltransferase Reaction through an Inhibition of Its Inherent Sialidase Activity by Dephosphorylation of Cytidine-5'-Monophosphate. [PDF]
PLoS ONE, 2015 Bacterial α(2,6)-sialyltransferases (STs) from Photobacterium damsela, Photobacterium sp. JT-ISH-224, and P. leiognathi JT-SHIZ-145 were recombinantly expressed in Escherichia coli and their ST activities were compared directly using a galactosylated bi ...
Ji-Yeon Kang +5 more
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Aberrant N-glycolylneuraminic acid in breast MCF-7 cancer cells and cancer stem cells
Frontiers in Molecular Biosciences, 2022 N-Glycolylneuraminic acid (Neu5Gc) is not normally detected in humans because humans lack the hydroxylase enzyme that converts cytidine-5′-monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac) to CMP-Neu5Gc; thus, any Neu5Gc appearing in the human body is ...
Wenqian Yang +4 more
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Molecular characterization of blood type A, B, and C (AB) in domestic cats and a CMAH genotyping scheme. [PDF]
PLoS ONE, 2018 In domestic cats, the AB blood group system consists of the three types A, B, and C (usually called AB), which vary in frequency among breeds and geographic regions.
Alexandra Kehl +6 more
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N-glycans: phenotypic homology and structural differences between myocardial cells and induced pluripotent stem cell-derived cardiomyocytes. [PDF]
PLoS ONE, 2014 Cell surface glycans vary widely, depending on cell properties. We hypothesized that glycan expression on induced pluripotent stem cells (iPSCs) might change during cardiomyogenic differentiation toward the myocardial phenotype.
Takuji Kawamura +13 more
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Comparison of lipooligosaccharides from human challenge strains of Neisseria gonorrhoeae
Frontiers in Microbiology, 2023 The alarming rise of antibiotic resistance and the emergence of new vaccine technologies have increased the focus on vaccination to control gonorrhea. Neisseria gonorrhoeae strains FA1090 and MS11 have been used in challenge studies in human males.
Constance M. John +6 more
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Frontiers in Veterinary Science, 2021 The feline AB blood group system (blood types A, B, and AB) encoding the cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH) gene is the most significant in transfusion medicine and hemolysis of the newborn for cats.
Yumiko Uno +10 more
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Multi-Enzymatic Cascade One-Pot Biosynthesis of 3′-Sialyllactose Using Engineered Escherichia coli
Molecules, 2020 Among the human milk oligosaccharides (HMOs), one of the most abundant oligosaccharides and has great benefits for human health is 3′-sialyllactose (3′-SL).
Zhongkui Li +5 more
doaj +1 more source
Ferrets exclusively synthesize Neu5Ac and express naturally humanized influenza A virus receptors [PDF]
, 2014 Mammals express the sialic acids N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) on cell surfaces, where they act as receptors for pathogens, including influenza A virus (IAV). Neu5Gc is synthesized from Neu5Ac by the enzyme
Böhm, Raphael +14 more
core +1 more source
Frontiers in Immunology, 2018 Genetically modified pigs have been considered favorable resources in xenotransplantation. Microinjection of randomly integrating transgenes into zygotes, somatic cell nuclear transfer, homologous recombination, zinc finger nucleases, transcription ...
Meisam Naeimi Kararoudi +7 more
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