Results 61 to 70 of about 2,416 (164)

L. pneumophila CMP-5,7-di- N -acetyllegionaminic acid synthetase (LpCLS)-involved chemoenzymatic synthesis of sialosides and analogues [PDF]

, 2020
5,7-Di-N-acetyllegionaminic acid (Leg5,7Ac2) is a bacterial nonulosonic acid (NulO) analogue of sialic acids, an important class of monosaccharides in mammals and in some bacteria. To develop efficient one-pot multienzyme (OPME) glycosylation systems for
Chen, Xi, Kooner, Anoopjit S, Li, Wanqing, Liu, Ziqi, McArthur, John B, Santra, Abhishek, Yu, Hai   +6 more
core   +1 more source

CMAH‐Targeted Knock‐In of Inducible Heme Oxygenase‐1 and Constitutive CD47 in GGTA1‐Knockout Pigs for Xenotransplantation

Xenotransplantation, Volume 33, Issue 2, March/April 2026.
ABSTRACT Background Pig xenografts offer a solution to human organ shortage; however, immune rejection is a major barrier. Immunomodulatory genes such as heme oxygenase‐1 (HO1) and CD47 are key. Precise promoter control and strategic genomic integration for reliable expression and xenoantigen disruption are critical.
Haesun Lee, Sang Eun Kim, Won Kil Lee, Miae Oh, Seunghoon Lee, Jin‐Gu No, Mi‐Ryung Park, Seokho Kim, Min Hwa Do, Keon Bong Oh   +9 more
wiley   +1 more source

Catch, Cut, or Block? Versatile 4‐N‐Derivatized Sialyl Glycosides for Influenza Virus Neuraminidase Detection and Purification

Angewandte Chemie, Volume 137, Issue 25, June 17, 2025.
Sialyl glycosides containing 4‐N‐derivatized sialic acids synthesized by a highly efficient one‐pot two‐enzyme (OP2E) chemoenzymatic sialylation strategy have been shown to be versatile probes. They can resist sialidase cleavage, be selective substrates by influenza sialidases, or be nanomolar substrate analog‐based inhibitors and affinity ligands ...
Yue Yuan, Hai Yu, Anand Kumar Agrahari, Jin Gao, Hyeog Kang, Robert Daniels, Xi Chen   +6 more
wiley   +2 more sources

Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection [PDF]

, 2023
Background and Aim: We previously developed the gene-editing by electroporation (EP) of Cas9 protein method, in which the CRISPR/Cas9 system was introduced into porcine in vitro fertilized (IVF) zygotes through EP to disrupt a target gene.
Do, Lanh Thi Kim, Hirata, Maki, Le, Quynh Anh, Lin, Qingyi, Namula, Zhao, Otoi, Takeshige, Takebayashi, Koki, Tanihara, Fuminori, Wittayarat, Manita   +8 more
core  

Donor‐Derived Cell‐Free DNA (dd‐cfDNA) as an Early Noninvasive Biomarker of Graft Injury in Pig‐to‐Monkey Islet Xenotransplantation

Xenotransplantation, Volume 33, Issue 2, March/April 2026.
ABSTRACT Background In pig‐to‐nonhuman primate islet transplantation, reliable, sensitive biomarkers are needed to detect graft damage at an early stage before irreversible islet loss occurs. In our study, we investigated donor‐derived cell‐free DNA (dd‐cfDNA) as an early, noninvasive biomarker of graft injury by analyzing its correlation with porcine ...
Ji‐Jing Yan, Jong‐Min Kim, Sang‐Ik Cho, Hyori Kim, Kyungmin Kwak, Hyunil Kim, Eun‐Jee Oh, Jaeseok Yang, Chung‐Gyu Park, Jong Cheol Jeong, Beom Seok Kim   +10 more
wiley   +1 more source

Absence of Neu5Gc and Presence of Anti-Neu5Gc Antibodies in Humans-An Evolutionary Perspective. [PDF]

, 2019
The glycocalyx of human cells differs from that of many other mammals by the lack of the sialic acid N-glycolylneuraminic acid (Neu5Gc) and increased abundance of its precursor N-acetylneuraminic acid (Neu5Ac).
Altman, Meghan O, Gagneux, Pascal
core   +1 more source

Microglia Display Altered Spatial Morphology and Proteome After Stroke

PROTEOMICS, Volume 25, Issue 23, Page 59-76, December 2025.
ABSTRACT Microglia are abundantly distributed throughout the central nervous system (CNS) to play critical roles in neural development and homeostasis, and act as immune sentinels to constantly monitor their surrounding neural environment. Given their high reactivity to brain insults, we hypothesised that the cerebral microenvironment altered by ...
Brooke J. Wanrooy, Jenny L. Wilson, Althea R. Suthya, Joshua H. Bourne, Joel R. Steele, Hossein Valipour Kahrood, Ralf B. Schittenhelm, Giulia Ballerin, Cameron Skinner, Shu Wen Wen, Connie H. Y. Wong   +10 more
wiley   +1 more source

lec32 Is a New Mutation in Chinese Hamster Ovary Cells That Essentially Abrogates CMP-N-acetylneuraminic Acid Synthetase Activity (*)

Journal of Biological Chemistry, 1995
LEC29.Lec32 is a glycosylation mutant that was isolated from a selection of mutagenized Chinese hamster ovary (CHO) cells for lectin resistance. Compared with LEC29 CHO cells, the double mutant exhibited an unusually high sensitivity to the toxic lectin,
B. Potvin, T. S. Raju, P. Stanley
semanticscholar   +1 more source

Neisseria gonorrhoeae scavenges host sialic acid for Siglec-mediated, complement-independent suppression of neutrophil activation

mBio
Gonorrhea, caused by the bacterium Neisseria gonorrhoeae (Gc), is characterized by neutrophilic influx to infection sites. Gc has developed mechanisms to resist killing by neutrophils that include modifications to its surface lipooligosaccharide (LOS ...
Amaris J. Cardenas, Keena S. Thomas, Mary W. Broden, Noel J. Ferraro, Marcos M. Pires, Constance M. John, Gary A. Jarvis, Alison K. Criss   +7 more
doaj   +1 more source

Study on the relationship between red meat, dietary Neu5Gc intake and anti-Neu5Gc antibody [PDF]

, 2015
唾液酸类化合物是以九碳糖神经氨酸(5-amino-3,5-dideoxy-D-glycero-D-galactononulsonicacid)为基本结构的一族衍生物的总称。目前，已分离和鉴定的有50余种，包括N-乙酰基神经氨酸(N-acetylneuraminicacid,Neu5Ac)、N-羟乙酰基神经氨酸(N-glycoulylneuraminicacid,Neu5Gc)和2－酮基－3－脱氧九酮糖酸(2-keto-3-deoxy-nonulosonicacid,KDN)三种核心单体 ...
范杏丹
core