Abstract Cytochalasin B (CB) at concentrations as low as 1 μg/ml inhibits phagocytosis of opsonized sheep erythrocytes by peritoneal macrophages. However, 50 μg/ml CB has no discernible effect on the pinocytosis of proteins. In the continuing presence of CB ingested proteins are degraded and released from macrophages in an apparently normal fashion ...
Fred R. Butcher, James F. Perdue
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CELL SORTING IN THE PRESENCE OF CYTOCHALASIN B [PDF]
The ability of cytochalasin B to inhibit ruffled membrane activity and cellular locomotion of vertebrate cells in monolayer culture prompted its use to study the necessity for this kind of active cellular locomotion in cell sorting in heterotypic cell aggregates.
Armstrong, Peter B, Parenti, David
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Reversibility of cell surface label rearrangement [PDF]
Cell surface labeling can cause rearrangements of randomly distributed membrane components. Removal of the label bound to the cell surface allows the membrane components to return to their original random distribution, demonstrating that label is ...
Brown, Susan Smith, Revel, Jean-Paul
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The Human Fungal Pathogen Cryptococcus neoformans Escapes Macrophages by a Phagosome Emptying Mechanism That Is Inhibited by Arp2/3 Complex-Mediated Actin Polymerisation [PDF]
The lysis of infected cells by disease-causing microorganisms is an efficient but risky strategy for disseminated infection, as it exposes the pathogen to the full repertoire of the host's immune system.
A Casadevall+36 more
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Occurrence and a possible mechanism of penetration of natural killer cells into k562 target cells during the cytotoxic interaction [PDF]
The cytotoxic interaction between cloned human Natural Killer (NK) cells and K562 target cells was studied using confocal laser scanning microscopy (CLSM) and conventional fluorescence microscopy.
de Grooth, B.G.+6 more
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In vitro markers for virulence in Yersinia ruckeri [PDF]
In this study, different traits that have been associated with bacterial virulence were studied in Yersinia ruckeri. Two isolates that had been shown to cause disease and mortality in experimentally infected rainbow trout were compared with five ...
Chiers, Koen+5 more
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Trogocytosis by Entamoeba histolytica Mediates Acquisition and Display of Human Cell Membrane Proteins and Evasion of Lysis by Human Serum. [PDF]
We previously showed that Entamoeba histolytica kills human cells through a mechanism that we termed trogocytosis ("trogo-" means "nibble"), due to its resemblance to trogocytosis in other organisms. In microbial eukaryotes like E.
Miller, Hannah W+2 more
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On the dynamics of the microfilament system in HeLa cells [PDF]
We measured the pools of unpolymerized and filamentous actin in homogenates of HeLa cells made in several different lysis buffers, as well as after treatment of cells with a variety of chemicals or trypsin, and after adenovirus (type 2) infection.
Blikstad, Ingrid, Carlsson, Lars
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Quantitative image mean squared displacement (iMSD) analysis of the dynamics of profilin 1 at the membrane of live cells. [PDF]
Image mean square displacement analysis (iMSD) is a method allowing the mapping of diffusion dynamics of molecules in living cells. However, it can also be used to obtain quantitative information on the diffusion processes of fluorescently labelled ...
Davey, Rhonda J+3 more
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Examination of actin and microtubule dependent APC localisations in living mammalian cells [PDF]
(provisional) Background The trafficking of the adenomatous polyposis coli (APC) tumour suppressor protein in mammalian cells is a perennially controversial topic. Immunostaining evidence for an actin-associated APC localisation at intercellular
Adams, A.+4 more
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