Frontiers in Cellular and Infection Microbiology, 2022 Although it is widely recognized that disruption of ALS3 reduces the invasion of Candida albicans germ tubes into mammalian oral epithelial cells, the mechanism of this interaction was unexplored. C.
Soon-Hwan Oh, Lois L. Hoyer
doaj +1 more source
On the dynamics of the microfilament system in HeLa cells [PDF]
, 1982 We measured the pools of unpolymerized and filamentous actin in homogenates of HeLa cells made in several different lysis buffers, as well as after treatment of cells with a variety of chemicals or trypsin, and after adenovirus (type 2) infection.
Blikstad, Ingrid, Carlsson, Lars
core +3 more sources
Comparison of whole blood versus red blood cells and plasma to correct trauma-induced coagulopathy ex vivo. [PDF]
TransfusionAbstract Background Early resuscitation is based on platelet‐poor components such as red blood cells and plasma (RBC + P), contributing to platelet dilution and worsening of trauma‐induced coagulopathy (TIC). We aimed to compare the ability of cold‐stored whole blood (WB) versus RBC + P as a single component to correct TIC.
Rossetto A +5 more
europepmc +2 more sources
The kinetics of cytochalasin D binding to monomeric actin. [PDF]
Journal of Biological Chemistry, 1986 It has been shown previously, using G-actin labeled with N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylene-diamine, that Mg2+ induces a conformational change in monomeric G-actin as a consequence of binding to a tight divalent cation binding site (Frieden, C. (1982) J. Biol. Chem. 257, 2882-2886).
Dean Goddette, Carl Frieden
openaire +2 more sources
α1A-adrenergic receptor induces activation of extracellular signal-regulated kinase 1/2 through endocytic pathway. [PDF]
PLoS ONE, 2011 G protein-coupled receptors (GPCRs) activate mitogen-activated protein kinases through a number of distinct pathways in cells. Increasing evidence has suggested that endosomal signaling has an important role in receptor signal transduction.
Fei Liu +8 more
doaj +1 more source
The Human Fungal Pathogen Cryptococcus neoformans Escapes Macrophages by a Phagosome Emptying Mechanism That Is Inhibited by Arp2/3 Complex-Mediated Actin Polymerisation [PDF]
, 2010 The lysis of infected cells by disease-causing microorganisms is an efficient but risky strategy for disseminated infection, as it exposes the pathogen to the full repertoire of the host's immune system.
A Casadevall +36 more
core +3 more sources
Stem Cells International, 2018 TGFβ is a potent regulator of several biological functions in many cell types, but its role in the differentiation of human bone marrow-derived skeletal stem cells (hMSCs) is currently poorly understood. In the present study, we demonstrate that a single
Mona Elsafadi +12 more
doaj +1 more source
Examination of actin and microtubule dependent APC localisations in living mammalian cells [PDF]
, 2006 (provisional) Background The trafficking of the adenomatous polyposis coli (APC) tumour suppressor protein in mammalian cells is a perennially controversial topic. Immunostaining evidence for an actin-associated APC localisation at intercellular
Adams, A. +4 more
core +3 more sources
Continuous, label-free, 96-well-based determination of cell migration using confluence measurement
Cell Adhesion & Migration, 2019 Cellular migration is essential in diverse physiological and pathophysiological processes. Here, we present a protocol for quantitative analysis of migration using confluence detection allowing continuous, non-endpoint measurement with minimal hands-on ...
Christian Mayr +10 more
doaj +1 more source
Trogocytosis by Entamoeba histolytica Mediates Acquisition and Display of Human Cell Membrane Proteins and Evasion of Lysis by Human Serum. [PDF]
, 2019 We previously showed that Entamoeba histolytica kills human cells through a mechanism that we termed trogocytosis ("trogo-" means "nibble"), due to its resemblance to trogocytosis in other organisms. In microbial eukaryotes like E.
Miller, Hannah W +2 more
core +1 more source