Results 51 to 60 of about 5,512,194 (292)

Spatial heterogeneity of hotspot mutations and clonal relatedness in ovarian endometrioid carcinoma

Molecular Oncology, EarlyView.
Spatially resolved analysis revealed heterogeneous distributions of selected cancer‐associated mutations within ovarian endometrioid carcinomas. Shared mutations between paired endometrial and ovarian tumors supported clonal relatedness. These findings highlight the value of sampling multiple tumor regions when interpreting molecular profiles ...
Takahito Ashihara, Kosuke Murakami, Shiro Takamatsu, Akiko Kanto, Seiichi Mori, Tomoyuki Otani, Kazuko Sakai, Yasushi Kotani, Kazuto Nishio, Kaoru Abiko, Noriomi Matsumura   +10 more
wiley   +1 more source

Beta1 subunits enable fentanyl‐induced BK channel activation and opioid regulation of cerebral artery diameter

FEBS Open Bio, EarlyView.
During the current opioid crisis, the majority of overdose deaths are attributed to ultra‐potent synthetic opioids, such as fentanyl. While fentanyl exerts its action via opioid receptors, we propose that potassium channels in the brain blood vessels may also sense fentanyl.
Shiwani Thapa, Mohammed Nooh, Akhil Geddati, Alex Dopico, Anna N. Bukiya   +4 more
wiley   +1 more source

Uncommon <em>EGFR</em> mutations in cytological specimens of 1,874 newly diagnosed Indonesian lung cancer patients [PDF]

, 2018
Purpose: We aimed to evaluate the distribution of individual epidermal growth factor receptor (EGFR) mutation subtypes found in routine cytological specimens.
Michael Levi, -, Laksmi Wulandari, Sabrina Ermayanti, Elisna Syahruddin, -, Sabrina Ermayanti, -, Michael Levi, Heriawaty Hidajat, -, Nunuk Sri Muktiati, Ana Rima, -, Nunuk Sri Muktiati, -, Ahmad Utomo, Laksmi Wulandari, -, Noni Soeroso, -, Heriawaty Hidajat, Grace Widjajahakim, -, Noni Soeroso, Elisna Syahruddin, Ana Rima, Ahmad Rusdan Handoyo Utomo, -, Grace Widjajahakim   +19 more
core   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

Analysis of reasons impairing the quality of cerebrospinal fluid cell slide centrifugal sedimentation examination method and its preventive measures

Chinese Journal of Contemporary Neurology and Neurosurgery, 2018
It is well known that cerebrospinal fluid (CSF) cell slide centrifugal sedimentation examination method is a very important technique for the etiological diagnosis of many central nervous system (CNS) diseases, especially for infectious diseases ...
Xiu-chu SU
doaj   +1 more source

Longitudinal Morphomolecular Monitoring of Head and Neck Carcinogenesis

Advanced Science, EarlyView.
A miniaturized endoscopic probe that integrates Raman spectroscopy and optical coherence tomography, enabling simultaneous molecular and structural imaging of living tissue, was developed. Applied longitudinally in a mouse model of head and neck cancer, the system tracks disease progression from precancerous lesions to invasive cancer.
Jianrong Qiu, Priyanka G. Bhosale, Vishal Kumar, Jeyrroy Gabriel, Shiyue Liu, Ciro Chiappini, Richard J. Cook, Mads S. Bergholt   +7 more
wiley   +1 more source

A Machine Vision‐Guided Microphysiological Platform With Automated Microfluidics Enables Longitudinal Biomarker Monitoring and Emulation of Translationally Relevant Exposure Scenarios

Advanced Science, EarlyView.
A pneumatically actuated multi‐tissue microphysiological system is integrated with AI‐based machine vision and automatic sampling and replenishment systems. The platform allows for the emulation of translationally relevant long‐term pharmacokinetic exposure scenarios for multiple weeks while enabling longitudinal monitoring of response biomarkers ...
Jibbe Keulen, Yi Zhong, Laura Dangel, Sonia Youhanna, Reza Zandi Shafagh, Jinhye Ryu, Domnica‐Gabriela Gurau, Mathias Haag, Thomas E. Mürdter, Yingxin Liang, Iro Beligiani, Nicole Ziegler, Sabine Willems, Nayere Taebnia, Wouter van der Wijngaart, Volker M. Lauschke   +15 more
wiley   +1 more source

Rapid KRAS, EGFR, BRAF and PIK3CA mutation analysis of fine needle aspirates from non-small-cell lung cancer using allele-specific qPCR. [PDF]

PLoS ONE, 2011
Endobronchial Ultrasound Guided Transbronchial Needle Aspiration (EBUS-TBNA) and Trans-esophageal Ultrasound Scanning with Fine Needle Aspiration (EUS-FNA) are important, novel techniques for the diagnosis and staging of non-small cell lung cancer (NSCLC)
Ronald van Eijk, Jappe Licht, Melanie Schrumpf, Mehrdad Talebian Yazdi, Dina Ruano, Giusi I Forte, Petra M Nederlof, Maud Veselic, Klaus F Rabe, Jouke T Annema, Vincent Smit, Hans Morreau, Tom van Wezel   +12 more
doaj   +1 more source

Multitask Deep Learning Model for Predicting Lymph Node Metastasis and Disease‐Free Survival From Cytology Images in Bladder Cancer: A Multicenter Study With Pan‐Cytology Validation

Advanced Science, EarlyView.
Through self‐supervised pretraining and supervised fine‐tuning on large‐scale cytology image datasets, CytoNet predicts lymph node metastasis and survival. Across independent cohorts, this multitask framework demonstrates robust performance, improves risk stratification beyond radiological assessment, and provides interpretable evidence to support ...
Guibin Hong, Fan Jiang, Ye Xie, Xiaowei Huang, Runnan Shen, Yun Wang, Mengyi Zhu, Haoxuan Wang, Hongkun Yang, Yanxiao Nie, Zhen Lin, Rui Chen, Nengtai Ouyang, Tianxin Lin, Shaoxu Wu   +14 more
wiley   +1 more source

Challenges of ICC and FISH in the Field of Targeted Therapies from Cell Block to Smears

Journal of Molecular Pathology, 2021
In the era of personalized medicine, there is an increasing demand for comprehensive and complex diagnosis using minimally invasive techniques. Nowadays, it is mandatory to integrate biomarkers in the diagnostic process, as well as in the treatment and ...
Jose I. Echeveste, Tania Labiano, Eva Tejerina, Allan Argueta, Carlos de Andrea, Maria D. Lozano   +5 more
doaj   +1 more source