Results 161 to 170 of about 1,366,500 (340)
Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs
FEBS Open Bio, EarlyView.Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner +7 more
wiley +1 more source
The Ratio Mitochondria-Cytoplasm in Normal and Tumor Cells
, 1962 A new technique for quantitative study of mitochondria is proposed. This method is based on the measurement by a photometric system of the area of the mitochondria and of the cytoplasm on electron microscopic photographs.
Marialuisa Melli
core +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
AMPs localize to cytoplasm-enriched fractions of NTHI.
, 2013 NTHI was fractionated to obtain cytoplasm and periplasm-enriched fractions. (A) Two dimensional gel electrophoresis of enriched fractions confirmed the presence of unique proteins in the periplasm and cytoplasm-enriched fractions.
Wandy L. Beatty (111795) +4 more
core +1 more source
FEBS Open Bio, EarlyView.In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara +3 more
wiley +1 more source
Characterizing the states of yeast cytoplasm using single particle tracking
, 2007 The cytoplasmic states of yeast cytoplasm have been characterized using differential interference contrast microscopy and high resolution single particle tracking.
Ibeneche, Chieze Chinenye
core +1 more source
Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1
FEBS Open Bio, EarlyView.The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay +2 more
wiley +1 more source
Stacks of mFV in the central cytoplasm of umbrella cells.
, 2012 (A) Cytoplasm of umbrella cells is divided into two regions: sub-apical (SA) and central (CE). Central cytoplasm contains majority of mFVs and other organelles. The border between the sub-apical and central cytoplasm is shown by a dashed line. (B) In the
Margit Pavelka (179422) +4 more
core +1 more source
Small RNA pathways in mammalian oocytes
FEBS Open Bio, EarlyView.Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley +1 more source
Mechanics and dynamics of living mammalian cytoplasm
, 2017 Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2017.Cataloged from PDF version of thesis.Includes bibliographical references (pages 53-58).Passive microrheology, a method of calculating the frequency dependent ...
Gupta, Satish Kumar, Ph.D. Massachusetts Institute of Technology
core