Results 21 to 30 of about 52,861 (267)

A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing

Nature Biotechnology, 2020
Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR–Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can ...
Julian Grünewald, Ronghao Zhou, C. Lareau, S. Garcia, Sowmya Iyer, Bret R. Miller, Lukas M. Langner, Jonathan Y. Hsu, Jonathan Y. Hsu, Martin J. Aryee, J. Joung   +10 more
semanticscholar   +1 more source

Hydrogen-bonding patterns in bis(cytosinium) tartarate monohydrate

IUCrData, 2017
The asymmetric unit of the title cystosinium salt derivative, 2C4H6N3O+·C4H4O62−·H2O, contains two cytosinium cations, one tartaric acid anion and a water molecule. The two cytosinium cations are almost planar (r.m.s. deviations of the fitted atoms are 0.
P. Jaikumar, T. V. Sundar, N. Sharmila, T. Balakrishnan, K. Ramamurthi   +4 more
doaj   +1 more source

Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice

Science, 2019
Spotting off-targets from gene editing Unintended genomic modifications limit the potential therapeutic use of gene-editing tools. Available methods to find off-targets generally do not work in vivo or detect single-nucleotide changes.
Shuai Jin, Y. Zong, Q. Gao, Zixu Zhu, Yanpeng Wang, P. Qin, Chengzhi Liang, Daowen Wang, J. Qiu, Feng Zhang, Caixia Gao   +10 more
semanticscholar   +1 more source

Comprehensive genetic screening for vascular Ehlers–Danlos syndrome through an amplification‐based next‐generation sequencing system

American Journal of Medical Genetics Part A, Volume 191, Issue 1, Page 37-51, January 2023., 2023
Abstract Vascular Ehlers–Danlos syndrome (vEDS) is a hereditary connective tissue disorder (HCTD) characterized by arterial dissection/aneurysm/rupture, sigmoid colon rupture, or uterine rupture. Diagnosis is confirmed by detecting heterozygous variants in COL3A1.
Tomomi Yamaguchi, Shujiro Hayashi, Daisuke Hayashi, Takeshi Matsuyama, Norimichi Koitabashi, Kenichi Ogiwara, Masaaki Noda, Chiai Nakada, Shinya Fujiki, Akira Furutachi, Yasuhiko Tanabe, Michiko Yamanaka, Aki Ishikawa, Miyako Mizukami, Asako Mizuguchi, Kazumitsu Sugiura, Makoto Sumi, Hirokuni Yamazawa, Atsushi Izawa, Yuko Wada, Tomomi Fujikawa, Yuri Takiguchi, Keiko Wakui, Kyoko Takano, Shin‐Ya Nishio, Tomoki Kosho   +25 more
wiley   +1 more source

Impact of cytosine methylation on DNA binding specificities of human transcription factors

Science, 2017
Positives and negatives of methylated CpG When the DNA bases cytosine and guanine are next to each other, a methyl group is generally added to the pyrimidine, generating a mCpG dinucleotide.
Yimeng Yin, E. Morgunova, A. Jolma, Eevi Kaasinen, Biswajyoti Sahu, Syed Khund-Sayeed, P. Das, Teemu Kivioja, Kashyap Dave, F. Zhong, K. Nitta, Minna Taipale, A. Popov, P. Ginno, Silvia Domcke, Jian Yan, D. Schübeler, C. Vinson, J. Taipale   +18 more
semanticscholar   +1 more source

Evaluating Relative Retention of Polar Stationary Phases in Hydrophilic Interaction Chromatography

Separations, 2019
A large number of polar stationary phases with diverse chemistry have been developed for various applications in hydrophilic interaction chromatography (HILIC).
Yong Guo, Nidhi Bhalodia, Bassel Fattal
doaj   +1 more source

To unwind the biological knots: The DNA/RNA G‐quadruplex resolvase RHAU (DHX36) in development and disease

Animal Models and Experimental Medicine, Volume 5, Issue 6, Page 542-549, December 2022., 2022
Schematic diagram showing RHAU can bind and unwind the G4 “knots” to modulate embryonic development and post‐natal organ function. Deficiency of RHAU gives rise to embryonic lethality, impaired organogenesis and organ dysfunction. RHAU modulates Nkx2‐5 mRNA and Yap1 mRNA at the post‐transcriptional level and facilitates the transcription of c‐Kit and ...
Chensi Yang, Jie Yao, Huijuan Yi, Xinyi Huang, Wukui Zhao, Zhongzhou Yang   +5 more
wiley   +1 more source

Detecting DNA cytosine methylation using nanopore sequencing

Nature Methods, 2017
In nanopore sequencing devices, electrolytic current signals are sensitive to base modifications, such as 5-methylcytosine (5-mC). Here we quantified the strength of this effect for the Oxford Nanopore Technologies MinION sequencer.
J. Simpson, J. Simpson, R. Workman, P. Zuzarte, Matei David, L. Dursi, W. Timp   +6 more
semanticscholar   +1 more source

Electron attachment to Cytosine: The Role of Water [PDF]

, 2020
We present an EOM-CCSD based QM/MM study on the electron attachment process to cytosine, solvated in water. The microhydration studies cannot capture the effect of bulk water environment on the electron attachment process and one need to include large number of water molecules in the calculation to get converged results.
arxiv   +1 more source

Use of the Illumina EPIC methylation array for epigenomic research in the crab‐eating macaque (Macaca fascicularis)

Neuropsychopharmacology Reports, 2020
Background Commercially available Illumina DNA methylation arrays (HumanMethylation 27K, HumanMethylation450, and MethylationEPIC BeadChip) can be used for comprehensive DNA methylation analyses of not only the human genome but also other mammalian ...
Yutaka Nakachi, Kazuhiro Ishii, Miki Bundo, Tomoyuki Masuda, Kazuya Iwamoto   +4 more
doaj   +1 more source