Results 21 to 30 of about 29,400,150 (101)

Toxicological Assessment of D-Allulose From a Novel One-Step Fermentation Process Using Genetically Modified <i>Escherichia coli</i>: A 90-Day Dietary Toxicity Study in Rats. [PDF]

J Toxicol
D‐Allulose is a rare monosaccharide structurally similar to D‐fructose, characterized by low caloric content and relatively high sweetness. This study aimed to conduct a 90‐day oral toxicity test to systemically evaluate the potential toxicological effects of D‐allulose produced via a novel one‐step fermentation process using genetically engineered ...
Li Z, Nie Y, Du H, Zhang W, Zheng S, Cong W, Feng Y, Li G, Jing H, Ning J, Gao S.   +10 more
europepmc   +2 more sources

Improved Performance of D-Psicose 3-Epimerase by Immobilisation on Amino-Epoxide Support with Intense Multipoint Attachment

Foods, 2021
D-allulose is an epimer of D-fructose at the C-3 position. With similar sweetness to sucrose and a low-calorie profile, D-allulose has been considered a promising functional sweetener.
Yifan Bu, Tao Zhang, Bo Jiang, Jingjing Chen   +3 more
doaj   +2 more sources

Safety of D-allulose as a novel food pursuant to Regulation (EU) 2015/2283. [PDF]

EFSA J
Abstract Following a request from the European Commission, the EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA) was asked to deliver an opinion on D‐allulose as a novel food (NF) pursuant to Regulation (EU) 2015/2283. During the risk assessment, the Panel identified a number of data gaps, which needed to be addressed by the applicant ...
EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA), Turck D, Cámara M, Bohn T, Castenmiller J, De Henauw S, Jos Á, Maciuk A, Mangelsdorf I, McNulty B, Naska A, Pentieva K, Siani A, Thies F, Aguilera-Gómez M, Cubadda F, Frenzel T, Heinonen M, Knutsen HK, McArdle HJ, Neuhäuser-Berthold M, Poulsen M, Maradona MP, Schlatter JR, Siskos A, van Loveren H, Multari S, Rossi A, Hirsch-Ernst KI.   +29 more
europepmc   +2 more sources

An Engineered Multi-Enzyme Cascade with Low-Cost ATP Regeneration for Efficient D-Allulose Production from D-Fructose

Fermentation
D-Allulose is a promising low-calorie rare sugar with significant health benefits. However, its industrial production is hindered by the low catalytic efficiency (≤33% conversion) and unfavorable equilibrium of the key enzyme, D-allulose 3-epimerase (DAE)
Yutong Lu, Huayang Tang, Dexun Fan, Qingzhu Wang, Shuangyan Han   +4 more
doaj   +2 more sources

Recent Advances Regarding the Physiological Functions and Biosynthesis of D-Allulose [PDF]

Frontiers in Microbiology, 2022
D-Allulose, a generally regarded as safe (GRAS) sugar, is rare in nature. It is among the most promising sweeteners for future use due to its low caloric content, sucrose-like taste, and unique functions.
Zhou Chen, Xiao-Dong Gao, Zijie Li
doaj   +2 more sources

One-site polarity switch enhances catalytic efficiency and stability of d-allulose 3-epimerase via flexibility–rigidity rebalancing [PDF]

Scientific Reports
The structural foundation of enzyme efficiency frequently resides in the subtle equilibrium between localized flexibility and overarching rigidity.
Fina Amreta Laksmi, Yudhi Nugraha, Kenny Lischer, Khaerunnisa, David Herawan, Ario Betha Juanssilfero, Des Saputro Wibowo, Isa Nuryana, Syahputra Wibowo, Keni Vidilaseris, Mulyorini Rahayuningsih   +10 more
doaj   +2 more sources

Gut microbial utilization of the alternative sweetener, D-allulose, via AlsE [PDF]

Communications Biology
D-allulose, a rare sugar with emerging potential as a low-calorie sweetener, has garnered attention as an alternative to other commercially available alternative sweeteners, such as sugar alcohols, which often cause severe gastrointestinal discomfort.
Glory Minabou Ndjite, Angela K. Jiang, Charlotte T. Ravel, Maggie R. Grant, Xiaofang Jiang, Brantley Hall   +5 more
doaj   +2 more sources

Thermostable D-Allulose 3-Epimerase for Long-Term Food-Compatible Continuous Production Systems

Applied Sciences
D-allulose is a rare sugar with promising applications in food and health industries, owing to its low caloric value and multiple health benefits.
Jiawei Cui, Yan Li, Ming Yan
doaj   +2 more sources

An Update: Enzymatic Synthesis for Industrial Applications. [PDF]

Angew Chem Int Ed Engl
Biocatalysis has become a sustainable and cost‐competitive alternative to established chemical synthesis, enabling the enzyme‐based production of not only commodity chemicals but (non‐natural) amino acids, (rare) sugars, as well as synthetic nucleotides.
Bayer T, Wu S, Snajdrova R, Baldenius K, Bornscheuer UT.   +4 more
europepmc   +2 more sources

Ancestral Sequence Reconstruction Enhances Thermal Stability of D-Allulose 3-Epimerase [PDF]

Shipin gongye ke-ji
To solve the problem of poor thermal stability of the current D-allulose 3-epimerase (DAEase), the ancestor sequences of DAEase with different catalytic domains were reconstructed by big data mining, reasonable modification and ancestor sequence ...
Lijun GUAN, Ling ZHU, Kunlun WANG, Jialei LI, Yang GAO, Song YAN, Xindi ZHANG, Qing CHEN, Nina JI, Bo LI   +9 more
doaj   +2 more sources