Results 41 to 50 of about 200 (72)

Research Progress Based on SpyTag/SpyCatcher Self‐Assembling Peptides and Their Applications in the Food Field

Food Bioengineering, Volume 4, Issue 3, Page 279-291, September 2025.
The formation of isopeptide bonds in the SpyTag/SpyCatcher system and its application in the food field ABSTRACT The SpyTag/SpyCatcher system is a modular protein assembly tool based on isopeptide bond‐mediated covalent conjugation, distinguished by mild reaction conditions, rapid ligation rate, and independence from exogenous enzymes or chemical ...
Meng‐meng Cai, Zi‐fu Ni, Zhong‐ke Sun, Xiao‐long Li, Zi‐hao Wang, Min Zhang, Cheng‐wei Li   +6 more
wiley   +1 more source

Improvement of D-Allulose Biocatalysis from D-Glucose in Engineered Escherichia coli by Enhancing Glucose Isomerase Expression and Substrate Supply

Fermentation
D-allulose is a rare low-calorie sugar with considerable health benefits and industrial potential. Compared with chemical synthesis and free enzyme catalysis, microbial production using engineered cells offers a low-cost and highly stable solution ...
Sheng Gao, Yinuo Li, Quan Cui, Chuanzhuang Guo, Jianbin Wang, Junlin Li, Ting Wang, Piwu Li, Jing Su, Ruiming Wang, Nan Li, Junqing Wang, Han Fan   +12 more
doaj   +1 more source

Update: Enzymatische Synthese für industrielle Anwendungen

Angewandte Chemie, Volume 137, Issue 27, July 1, 2025.
Die Biokatalyse hat sich zu einer nachhaltigen und wettbewerbsfähigen Alternative zur herkömmlichen chemischen Synthese entwickelt und ermöglicht die enzymbasierte Herstellung nicht nur von chemischen Grundstoffen, sondern auch von (nicht natürlichen) Aminosäuren, (seltenen) Zuckern und synthetischen Nukleotiden.
Thomas Bayer, Shuke Wu, Radka Snajdrova, Kai Baldenius, Uwe T. Bornscheuer   +4 more
wiley   +1 more source

Ancestral Sequence Reconstruction Enhances Thermal Stability of D-Allulose 3-Epimerase

Shipin gongye ke-ji
To solve the problem of poor thermal stability of the current D-allulose 3-epimerase (DAEase), the ancestor sequences of DAEase with different catalytic domains were reconstructed by big data mining, reasonable modification and ancestor sequence ...
Lijun GUAN, Ling ZHU, Kunlun WANG, Jialei LI, Yang GAO, Song YAN, Xindi ZHANG, Qing CHEN, Nina JI, Bo LI   +9 more
doaj   +1 more source

Study on Immobilization of Hyperthermophilic D-Allulose 3-Epimerase and Properties of Immobilized Enzyme(超嗜热D-阿洛酮糖3-差向异构酶的固定化及固定化酶性能研究) [PDF]

Shipin kexue jishu xuebao
D-allulose, a promising low-calorie natural sweetener and a key member of the rare sugar family, exhibits physiological benefits in mitigating multiple diseases.
SHEN Jidong(沈骥冬), XU Baoping(徐宝萍), HUANG Lianggang(黄良刚), LIU Zhiqiang(柳志强), ZHENG Yuguo(郑裕国)   +4 more
doaj   +1 more source

Learning from Protein Engineering by Deconvolution of Multi‐Mutational Variants

Angewandte Chemie International Edition, Volume 63, Issue 36, September 2, 2024.
First derivative of a typical experimental enzyme fitness pathway landscape mapped on the basis of ΔG values, in this case of 24 trajectories in the deconvolution of a 4‐mutational variant. Green areas indicate negative slopes and define trajectories involved in energetically favored stages, while red areas indicate positive slopes representing ...
Frank Hollmann, Joaquin Sanchis, Manfred T. Reetz   +2 more
wiley   +1 more source

Thermostable D-Allulose 3-Epimerase for Long-Term Food-Compatible Continuous Production Systems

Applied Sciences
D-allulose is a rare sugar with promising applications in food and health industries, owing to its low caloric value and multiple health benefits.
Jiawei Cui, Yan Li, Ming Yan
doaj   +1 more source

Preparation and Properties Analysis of D-Allulose-3-Epimerase Cross-linked Enzyme Aggregates

Shipin gongye ke-ji
This study aimed to immobilize D-allulose 3-epimerase from Ruminococcus sp. (RDAE) using cross-linked enzyme aggregates (CLEAs) technology. The immobilized enzyme (RDAE-CLEA) was prepared by optimizing the precipitant polyethylene glycol (PEG4000 ...
Ronghui QIU, Ziyi LI, Jiao LI, Depei WANG, Yan ZENG, Yuanxia SUN   +5 more
doaj   +1 more source

A Novel d-Allulose 3-Epimerase Gene from the Metagenome of a Thermal Aquatic Habitat and d-Allulose Production by Bacillus subtilis Whole-Cell Catalysis. [PDF]

Appl Environ Microbiol, 2020
Patel SN, Kaushal G, Singh SP.
europepmc   +1 more source

The Engineering, Expression, and Immobilization of Epimerases for D-allulose Production. [PDF]

Int J Mol Sci, 2023
Tan JH, Chen A, Bi J, Lim YH, Wong FT, Ow DS.   +5 more
europepmc   +1 more source