Construction and Optimization of Biosynthetic Pathway for the Production of D-Allulose from Glucose in Escherichia coli [PDF]
Shipin KexueA pathway in Escherichia coli BL21 (DE3) was constructed to produce D-allulose from D-glucose via a phosphorylation-dephosphorylation strategy. The genes related to competitive pathways were deleted using the CRISPR/Cas9 system, and allulose-6-phosphate
JIANG Yawen, ZHANG Cangping, YANG Shaoqing, JIANG Zhengqiang, LI Yanxiao, YAN Qiaojuan
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Gut microbial utilization of the alternative sweetener, D-allulose, via AlsE [PDF]
Communications BiologyD-allulose, a rare sugar with emerging potential as a low-calorie sweetener, has garnered attention as an alternative to other commercially available alternative sweeteners, such as sugar alcohols, which often cause severe gastrointestinal discomfort.
Glory Minabou Ndjite +5 more
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Biochemical characterization, structure-guided mutagenesis, and application of a recombinant D-allulose 3-epimerase from Christensenellaceae bacterium for the biocatalytic production of D-allulose [PDF]
Frontiers in Bioengineering and BiotechnologyD-Allulose has become a promising alternative sweetener due to its unique properties of low caloric content, moderate sweetness, and physiological effects. D-Allulose 3-epimerase (DAEase) is a promising enzyme for D-Allulose production.
Lijun Guan +22 more
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Efficient D-allulose synthesis under acidic conditions by auto-inducing expression of the tandem D-allulose 3-epimerase genes in Bacillus subtilis [PDF]
Microbial Cell Factories, 2022 Background D-allulose, a hexulose monosaccharide with low calorie content and high sweetness, is commonly used as a functional sugar in food and nutrition.
Mengkai Hu +7 more
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Effects of D-allulose on batter performance and quality of sponge cakes
Shipin yu jixieObjectiveTo investigate whether D-allulose can replace sucrose in sponge cakes.MethodsD-allulose is used as a sweetener to replace sucrose in the production of sponge cakes. The effects of different substitution levels of D-allulose(0%, 10%, 20%, 30%, 40%
CHAI Xiya +4 more
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Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 Rare sugars are regarded as functional biological materials due to their potential applications as low-calorie sweeteners, antioxidants, nucleoside analogs, and immunosuppressants.
Mi Na Choi +6 more
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D-allulose ameliorates adiposity through the AMPK-SIRT1-PGC-1α pathway in HFD-induced SD rats [PDF]
Food & Nutrition Research, 2021 Background: Adiposity is a major health-risk factor, and D-allulose has beneficial effects on adiposity-related metabolic disturbances. However, the modes of action underlying anti-hyperglycemic and hypolipidemic activity are partly understood. Objective:
Geum-Hwa Lee +9 more
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Applied Sciences, 2019 D-allulose, which has 70% of the sweet taste of sucrose but nearly no calories, has been reported to inhibit the absorption of lipids and suppress body weight gain in obese mice.
Ga Young Do +6 more
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Production of recombinant D-allulose 3-epimerase utilizing an auto-induction approach in fermentor cultures suitable for industrial application. [PDF]
PLoS ONED-Allulose 3-epimerase (DAEase) is the key enzyme catalyzing D-fructose to catalyze into D-allulose, a rare sugar in foods, which has lately drawn increasing worldwide attention owing to its possible health advantages and application as a substitute ...
Kenny Lischer +10 more
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Thermostable D-Allulose 3-Epimerase for Long-Term Food-Compatible Continuous Production Systems
Applied SciencesD-allulose is a rare sugar with promising applications in food and health industries, owing to its low caloric value and multiple health benefits.
Jiawei Cui, Yan Li, Ming Yan
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