Results 131 to 140 of about 408 (142)

Purification, immobilization, evolution, and characterization of D-allulose 3-epimerase from Antarctic Microbacterium

International Journal of Biological Macromolecules
D-allulose is a rare, low-calorie sugar substitute with multiple physiological functions and is widely used in food and pharmaceutical industries. D-allulose is primarily produced by the catalytic conversion of d-fructose via D-allulose 3-epimerase (DAEase). In this study, a DAEase gene, dpema4, was isolated from an Antarctic bacterium and expressed in
Jingqi, Li, Jingjing, Sun, Wei, Wang, Chengcheng, Jiang, Jianhua, Hao   +4 more
openaire   +2 more sources

Identification of hyperthermophilic D-allulose 3-epimerase from Thermotoga sp. and its application as a high-performance biocatalyst for D-allulose synthesis

Bioprocess and Biosystems Engineering
D-Allulose 3-epimerase (DAE) is a vital biocatalyst for the industrial synthesis of D-allulose, an ultra-low calorie rare sugar. However, limited thermostability of DAEs hinders their use at high-temperature production. In this research, hyperthermophilic TI-DAE (Tm = 98.4 ± 0.7 ℃) from Thermotoga sp. was identified via in silico screening.
Ji-Dong, Shen, Bao-Ping, Xu, Te-Li, Yu, Yong-Xiang, Fei, Xue, Cai, Liang-Gang, Huang, Li-Qun, Jin, Zhi-Qiang, Liu, Yu-Guo, Zheng   +8 more
openaire   +2 more sources

Characterization of a recombinant d-allulose 3-epimerase from Agrobacterium sp. ATCC 31749 and identification of an important interfacial residue

International Journal of Biological Macromolecules, 2018
d-Allulose 3-epimerase (DAEase) catalyzes the epimerization between d-fructose and d-allulose. We had PCR-cloned and overexpressed the gene encoding Agrobacterium sp. ATCC 31749 DAEase (AsDAEase) in Escherichia coli. A high yield of active AsDAEase, 35,300U/L or 1350U/g of wet cells, was acquired with isopropyl β-d-1-thiogalactopyranoside induction at ...
Wen-Chi Tseng, Chao-Nan Chen, Chung-Ting Hsu, Hsu-Chieh Lee, Hong-Yi Fang, Ming-Jun Wang, Yi-Hung Wu, Tsuei-Yun Fang   +7 more
openaire   +2 more sources

Enhancing enzyme immobilization: Fabrication of biosilica-based organic-inorganic composite carriers for efficient covalent binding of D-allulose 3-epimerase

International Journal of Biological Macromolecules
D-allulose, an ideal low-calorie sweetener, is primarily produced through the isomerization of d-fructose using D-allulose 3-epimerase (DAE; EC 5.1.3.30). Addressing the gap in available immobilized DAE enzymes for scalable commercial D-allulose production, three core-shell structured organic-inorganic composite silica-based carriers were designed for ...
Ziqun, Xiao, Zishen, Zhao, Bo, Jiang, Jingjing, Chen   +3 more
openaire   +2 more sources

Secreted d-allulose 3-epimerase for rare d-allulose biosynthesis and yeast-assisted isomer removal from d-allulose

Food Bioscience
Xiaofang Xie, Zihang Jiang, Shixia Xi, Liyuan Jiang, Dejian Huang, Zhaofeng Li   +5 more
openaire   +1 more source

Structural and Functional Features of Ketose-3-Epimerases and Their Use for D-Allulose Production

Russian Journal of Bioorganic Chemistry, 2023
N. S. Ivanova, A. A. Kulminskaya, S. V. Shvetsova   +2 more
openaire   +1 more source

Surface activation of hierarchically porous diatomite modified with polyethyleneimine for immobilizing d-allulose 3-epimerase

Food Bioscience, 2023
Ziqun Xiao, Zishen Zhao, Bo Jiang, Jingjing Chen   +3 more
openaire   +1 more source

Immobilization and stabilization of D-allulose 3-epimerase for continuous D-allulose synthesis in packed-bed reactors

Food Bioscience
Ziqun Xiao, Bo Jiang, Jingjing Chen, Longbei Xiang, Ran Zhang   +4 more
openaire   +1 more source

Immobilization of cells expressing d-allulose 3-epimerase and their application in d-allulose bioproduction with the assistance of boric acid

Food Bioscience
Xin Wen, Huibin Lin, Guangwen Liu, Yuhang Ning, Yilin Ren, Can Li, Chengjia Zhang, Nannan Dong, Jianqiang Lin, Xin Song, Guoqiang Zhuang, Jianqun Lin   +11 more
openaire   +1 more source

A novel xylose isomerase suitable for D-fructose production and synergistic catalysis with D-allulose 3-epimerase for the biosynthesis of D-allulose

Process Biochemistry
Satya Narayan Patel, Sweety Sharma, Nidhi Gossai, Dhaval Patel, Sudhir Pratap Singh   +4 more
openaire   +1 more source