Results 141 to 150 of about 14,110,935 (199)

D‐psicose 3‐epimerase secretory overexpression, immobilization, and d‐psicose biotransformation, separation and crystallization

Journal of Chemical Technology & Biotechnology, 2018
AbstractBACKGROUNDD‐psicose is a rare sugar and exists in extremely small quantities in nature. It has important physiological functions and is allowed to be used as an ingredient in foods and dietary supplements. The aim of this study is to develop the biotransformation, separation and purification methods for highly efficient mass production of d ...
Can Li, Jian-qun Lin, Qingqing Guo, Chengjia Zhang, Kai Du, H. Lin, Jianqiang Lin   +6 more
semanticscholar   +2 more sources

High production of d‐psicose from d‐fructose by immobilized whole recombinant Bacillus subtilis cells expressing d‐psicose 3‐epimerase from Agrobacterium tumefaciens

Biotechnology and applied biochemistry, 2021
d‐Psicose 3‐epimerase (DPEase) can catalyze the isomerization of d‐fructose to be rare sugar d‐psicose, which has wide application prospects in the food and medical fields.
Jianwei Wang, Jiandong Sun, Hongqing Qi, Liang Wang, Jihui Wang, Cheng Li   +5 more
semanticscholar   +1 more source

Characterization of a d-psicose-producing enzyme, d-psicose 3-epimerase, from Clostridium sp.

Biotechnology Letters, 2013
The gene coding for D-psicose 3-epimerase (DPEase) from Clostridium sp. BNL1100 was cloned and expressed in Escherichia coli. The recombinant enzyme was purified by Ni-affinity chromatography. It was a metal-dependent enzyme and required Co(2+) as optimum cofactor.
Wanmeng, Mu, Wenli, Zhang, Dan, Fang, Leon, Zhou, Bo, Jiang, Tao, Zhang   +5 more
openaire   +2 more sources

Production of d -psicose from d -glucose by co-expression of d -psicose 3-epimerase and xylose isomerase

Enzyme and Microbial Technology, 2017
d-Psicose has been drawing increasing attention in recent years because of its medical and health applications. The production of d-psicose from d-glucose requires the co-expression and synergistic action of xylose isomerase and d-psicose 3-epimerase.
Xiaoyan, Chen, Wen, Wang, Jingliang, Xu, Zhenhong, Yuan, Tao, Yuan, Yu, Zhang, Cuiyi, Liang, Minchao, He, Ying, Guo   +8 more
openaire   +2 more sources

A stable immobilized d-psicose 3-epimerase for the production of d-psicose in the presence of borate

Process Biochemistry, 2009
Abstract Maximal activity of the immobilized d -psicose 3-epimerase from Agrobacterium tumefaciens on Duolite A568 beads was achieved at pH 9.0 and 55 °C with borate, and at pH 8.5 and 50 °C without borate. The half-lives of the immobilized enzyme at 50 °C with and without borate were increased 4.2- and 128-fold compared to that of the free enzyme ...
Byung-Chul Lim, Hye-Jung Kim, Deok-Kun Oh   +2 more
openaire   +1 more source

Overexpression of d-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in d-psicose production

Biotechnology Letters, 2012
The D-psicose 3-epimerase (DPE) gene from Ruminococcus sp. was cloned and overexpressed in Escherichia coli. The recombinant protein was purified and characterized. It was optimally active at pH 7.5-8.0 and 60 °C. Activity was not dependent on the presence of metal ions; however, it became more thermostable with added Mn(2+).
Yueming, Zhu, Yan, Men, Wei, Bai, Xiaobo, Li, Lili, Zhang, Yuanxia, Sun, Yanhe, Ma   +6 more
openaire   +2 more sources

A d-psicose 3-epimerase with neutral pH optimum from Clostridium bolteae for d-psicose production: cloning, expression, purification, and characterization

Applied Microbiology and Biotechnology, 2013
D-Tagatose 3-epimerase family enzymes can efficiently catalyze the epimerization of free keto-sugars, which could be used for D-psicose production from D-fructose. In previous studies, all optimum pH values of these enzymes were found to be alkaline. In this study, a D-psicose 3-epimerase (DPEase) with neutral pH optimum from Clostridium bolteae (ATCC ...
Min, Jia, Wanmeng, Mu, Feifei, Chu, Xiaoming, Zhang, Bo, Jiang, Liuming Leon, Zhou, Tao, Zhang   +6 more
openaire   +2 more sources

Co-expression of d-glucose isomerase and d-psicose 3-epimerase: Development of an efficient one-step production of d-psicose

Enzyme and Microbial Technology, 2014
D-Psicose has been attracting attention in recent years because of its alimentary activities and is used as an ingredient in a range of foods and dietary supplements. To develop a one-step enzymatic process of D-psicose production, thermoactive D-glucose isomerase and the D-psicose 3-epimerase obtained from Bacillus sp.
Yan, Men, Yueming, Zhu, Yan, Zeng, Ken, Izumori, Yuanxia, Sun, Yanhe, Ma   +5 more
openaire   +2 more sources

Recent advances on applications and biotechnological production of d-psicose

Applied Microbiology and Biotechnology, 2012
D-Psicose is a hexoketose monosaccharide sweetener, which is a C-3 epimer of D-fructose and is rarely found in nature. It has 70 % relative sweetness but 0.3 % energy of sucrose, and is suggested as an ideal sucrose substitute for food products. It shows important physiological functions, such as blood glucose suppressive effect, reactive oxygen ...
Wanmeng, Mu, Wenli, Zhang, Yinghui, Feng, Bo, Jiang, Leon, Zhou   +4 more
openaire   +2 more sources

Improved operational stability of d -psicose 3-epimerase by a novel protein engineering strategy, and d -psicose production from fruit and vegetable residues

Bioresource Technology, 2016
The aim of the present work was to improve stability of d-psicose 3-epimerase and biotransformation of fruit and vegetable residues for d-psicose production. The study established that N-terminal fusion of a yeast homolog of SUMO protein - Smt3 - can confer elevated optimal temperature and improved operational stability to d-psicose 3-epimerase.
Satya Narayan, Patel, Manisha, Sharma, Kusum, Lata, Umesh, Singh, Vinod, Kumar, Rajender S, Sangwan, Sudhir P, Singh   +6 more
openaire   +2 more sources