Results 1 to 10 of about 3,622,932 (286)

Replication dynamics of recombination-dependent replication forks

Nature Communications, 2021
Replication forks that are stalled at obstacles on the DNA template can be restarted by homologous recombination. Here, the authors show replication dynamics during homologous recombination-dependent replication fork restart by combining polymerase usage
Karel Naiman, Eduard Campillo-Funollet, Adam T. Watson, Alice Budden, Izumi Miyabe, Antony M. Carr   +5 more
doaj   +1 more source

HspBP1 is a dual function regulatory protein that controls both DNA repair and apoptosis in breast cancer cells

Cell Death and Disease, 2022
The Hsp70-binding protein 1 (HspBP1) belongs to a family of co-chaperones that regulate Hsp70 activity and whose biological significance is not well understood.
Cha Kyung Youn, Jung-Hee Lee, Gurusamy Hariharasudhan, Hong Beum Kim, Jeeho Kim, Sumi Lee, Sung-Chul Lim, Sang-Pil Yoon, Sang-Gon Park, In-Youb Chang, Ho Jin You   +10 more
doaj   +1 more source

Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1

eLife, 2023
Arrested replication forks, when restarted by homologous recombination, result in error-prone DNA syntheses and non-allelic homologous recombination. Fission yeast RTS1 is a model fork barrier used to probe mechanisms of recombination-dependent restart ...
Alice M Budden, Murat Eravci, Adam T Watson, Eduard Campillo-Funollet, Antony W Oliver, Karel Naiman, Antony M Carr   +6 more
doaj   +1 more source

CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptation

Nature Communications, 2021
CAPPs are putative Primase-Polymerases associated with CRISPR-Cas operons. Here, the authors show CAPPs genetic and physical association with Cas1 and Cas2, their capacity to function as DNA-dependent DNA primases and DNA polymerases, and that Cas1-Cas2 ...
Katerina Zabrady, Matej Zabrady, Peter Kolesar, Arthur W. H. Li, Aidan J. Doherty   +4 more
doaj   +1 more source

Inhibition of MRN activity by a telomere protein motif

Nature Communications, 2021
Telomeres suppress the DNA damage response at chromosome ends. Here the authors show that in budding yeast the activity of the MRX complex in DNA repair and DNA damage signaling is inhibited by telomeric protein Rif2 via a short motif at the N-terminus.
Freddy Khayat, Elda Cannavo, Majedh Alshmery, William R. Foster, Charly Chahwan, Martino Maddalena, Christopher Smith, Antony W. Oliver, Adam T. Watson, Antony M. Carr, Petr Cejka, Alessandro Bianchi   +11 more
doaj   +1 more source

Live-cell single-molecule tracking highlights requirements for stable Smc5/6 chromatin association in vivo

eLife, 2021
The essential Smc5/6 complex is required in response to replication stress and is best known for ensuring the fidelity of homologous recombination. Using single-molecule tracking in live fission yeast to investigate Smc5/6 chromatin association, we show ...
Thomas J Etheridge, Desiree Villahermosa, Eduard Campillo-Funollet, Alex David Herbert, Anja Irmisch, Adam T Watson, Hung Q Dang, Mark A Osborne, Antony W Oliver, Antony M Carr, Johanne M Murray   +10 more
doaj   +1 more source

A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome

Nature Communications, 2019
Topoisomerase 2 (Top2) is known to resolve DNA topological stress through double strand breaks (DSBs), yet Top2 inhibition has been reported to result in a significant amount of single-strand breaks (SSBs).
William H. Gittens, Dominic J. Johnson, Rachal M. Allison, Tim J. Cooper, Holly Thomas, Matthew J. Neale   +5 more
doaj   +1 more source

PLK1 facilitates chromosome biorientation by suppressing centromere disintegration driven by BLM-mediated unwinding and spindle pulling

Nature Communications, 2019
The kinase PLK1 has important roles during cell division, including mitotic entry and bipolar spindle formation. Here, the authors show that PLK1 also functions in centromere protection, with loss leading to DNA unwinding by Bloom’s syndrome helicase and
Owen Addis Jones, Ankana Tiwari, Tomisin Olukoga, Alex Herbert, Kok-Lung Chan   +4 more
doaj   +1 more source

Bone Marrow CD133 Stem Cells Ameliorate Visual Dysfunction in Streptozotocin-induced Diabetic Mice with Early Diabetic Retinopathy

Cell Transplantation, 2018
Diabetic retinopathy (DR), one of the leading causes of vision loss worldwide, is characterized by neurovascular disorders. Emerging evidence has demonstrated retinal neurodegeneration in the early pathogenesis of DR, and no treatment has been developed ...
Liyuan Rong, Xianliang Gu, Jing Xie, Yuxiao Zeng, Qiyou Li, Siyu Chen, Ting Zou, Langyue Xue, Haiwei Xu, Zheng Qin Yin   +9 more
doaj   +1 more source

Automation of the cytokinesis-block micronucleus cytome assay by laser scanning cytometry and its potential application in radiation biodosimetry

BioTechniques, 2014
Here we describe the adaptation of laser scanning cytometry (LSC) to measure micronuclei (MN) automatically in lymphocytes. MN frequencies were determined in irradiated human lymphocytes using the cytokinesis-block technique, and the results from LSC ...
Maxime François, Kevin Hochstenbach, Wayne Leifert, Michael Felix Fenech   +3 more
doaj   +1 more source