Results 71 to 80 of about 619,613 (312)
Wellcome Open Research, 2017 The use of constitutive promoters to drive exogenous protein expression is an important tool for the study of diverse biological processes. To create and characterise a stably integrated expression system for DT40 cells, we constructed integration ...
Meliti Skouteri +2 more
doaj +1 more source
Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Application of damage mechanics in percussive drilling modelling [PDF]
, 2005 A stationary-periodic quasi-static model of rock percussive deep drilling is introduced. First, an auxiliary problem of stationary indentation of a rigid indentor is considered.
Mikhailov, SE +3 more
core
Evolution of p-factors in the probabilistic damage stability assessment of passenger ships [PDF]
, 2022 The paper complements an earlier publication by the authors addressing the probability of survival in the IMO framework for damage stability assessment, the s-factor. The focus here is on the probability of occurrence of a certain damage scenario (breach)
Mujeeb Ahmed +4 more
core
Osmotic disruption of chromatin induces Topoisomerase 2 activity at sites of transcriptional stress
Nature CommunicationsTranscription generates superhelical stress in DNA that poses problems for genome stability, but determining when and where such stress arises within chromosomes is challenging. Here, using G1-arrested S.
William H. Gittens +4 more
doaj +1 more source
eLife, 2019 Coordination of the cellular response to DNA damage is organised by multi-domain ‘scaffold’ proteins, including 53BP1 and TOPBP1, which recognise post-translational modifications such as phosphorylation, methylation and ubiquitylation on other proteins ...
Nicolas Bigot +5 more
doaj +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Stability analysis of water-filled dump slope under the couple effect of seepage and damage [PDF]
, 2012 The stability problem of water-filled dump slope becomes one of the geotechnical engineering problems needed to be resolved. Especially, the couple effect of seepage and damage has a great influence on the dump slope.
吕祥锋 +3 more
core
DNA damage responses in the context of the cell division cycle [PDF]
, 2010 During my PhD, I have investigated aspects of the DNA damage response (DDR) in the context of three different cellular scenarios: DNA damage signalling in response to double-strand breaks during mitosis, coordination of DNA replication with DNA damage ...
core +2 more sources
Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source