Results 71 to 80 of about 92,055 (268)

Protein Structuromics Reveals a Loop‐Controlled Half‐Open Active Pocket Conformation Throughout Fe(II)/α‐ketoglutarate‐Dependent Dioxygenase Catalytic Cycle

Advanced Science, EarlyView.
This study reveals a conserved half‐open active pocket in Fe(II)/α‐ketoglutarate‐dependent dioxygenases through protein structuromics, distinguished by structural features comprising highly flexible loop elements. Enhanced sampling simulations‐guided mutagenesis experiments elucidated how this flexible loop regulates pocket open/closed conformations ...
Lunjie Wu, Huan Liu, Songyin Zhao, Lei Qin, Jiarui Li, Jun Wang, Zixuan Dai, Jie Gu, Yan Xu, Feiran Li, Yao Nie   +10 more
wiley   +1 more source

Single‐Cell Annotation and Localization via Integrating Spatial Transcriptomics Maps the Mouse Ocular Atlas and RAO Dynamics

Advanced Science, EarlyView.
We developed the ASCAL pipeline, integrating complementary spatial transcriptomics, to construct a high‐fidelity mouse whole‐eye single‐cell atlas. Applying ASCAL to a retinal artery occlusion (RAO) model revealed spatially restricted immune activation localized to the ganglion cell layer and the selective depletion of a translationally active, outer ...
Chen Du, Yinming Li, Ziyue Li, Yuedan Wang, Shaopeng Li, Yijie Wang, Yingying Qu, Bingyang Lv, Ying Li, Ting Chen, Yu Zhou, Xuan Xiao   +11 more
wiley   +1 more source

Integration between Bioinformatics Algorithms and Neutrosophic Theory

Neutrosophic Sets and Systems
This paper presents a neutrosophic inference model for bioinformatics. The model is used to develop a system for accurate comparisons of human nucleic acids, where the new nucleic acid is compared to a database of old nucleic acids.
Romany M. Farag, Mahmoud Y. Shams, Dalia A. Aldawody, Huda E. Khalid, Hazem M. El-Bakry, Ahmed A. Salama   +5 more
doaj   +1 more source

ProSiteHunter: A Unified Framework for Sequence‐Based Prediction of Protein‐Nucleic Acid and Protein‐Protein Binding Sites

Advanced Science, EarlyView.
This study proposed a unified sequence‐based framework for protein binding site prediction, which adopted a tri‐track semantic multi‐source feature fusion strategy to effectively capture diverse macromolecular interaction sites and further improved the accuracy of antibody‐antigen interaction prediction.
Dongliang Hou, Qihang Zhen, Zexin Lv, Xinyue Cui, Suhui Wang, Minghua Hou, Zhan Zhou, Xiaogen Zhou, Guijun Zhang   +8 more
wiley   +1 more source

Molecular assays and point-of-care tools: a review of amplification methods and nucleic acid detection systems [PDF]

Anais da Academia Brasileira de Ciências
Molecular diagnosis is crucial for combating infectious diseases due to its high sensitivity and precision. However, its application in point-of-care testing (POCT) faces challenges, particularly in remote areas and developing countries.
VALTEMAR F. CARDOSO, CAIO C. DE SOUZA, JULIANE C. GLÓRIA, ELIZA RAQUEL D. DA SILVA, ARIAMNA MARÍA DIP GANDARILLA, WALTER RICARDO BRITO, LUIS ANDRÉ M. MARIÚBA   +6 more
doaj   +2 more sources

A Dual‐Phase Exosomal Nanotherapy Enhances Continual Efferocytosis by Coordinating Checkpoint Inhibition and Metabolic Reprogramming in Atherosclerosis

Advanced Science, EarlyView.
Here we developed Am@SExo, a dual‐functional engineered exosome that coordinates CD47–SIRPα checkpoint blockade with Arg1 mRNA–mediated metabolic reprogramming. Surface SIRPα promotes recognition of apoptotic cells and plaque targeting, while Arg1 expression enhances arginine–ornithine metabolism, Rac1 activation, and actin remodeling in macrophages ...
Danwen Zheng, Shiteng Cai, Jinfeng Gao, Sheng Zhao, Bohan Wei, Xueyi Weng, Zhengmin Wang, Qiaozi Wang, Qiyu Li, Chengzhi Han, Weiyan Li, Yiwen Tan, Yuyuan Fu, Meng Ji, Zheyong Huang, Yanan Song, Juying Qian, Junbo Ge   +17 more
wiley   +1 more source

Synergistic HMGN1 and VP64 Fusions Potentiate High‐Precision and PAM‐Flexible Base Editing

Advanced Science, EarlyView.
A novel CDA1Δ‐SpRY architecture fused with HMGN1 and VP64 yields a nearly PAM‐less base editing platform. By focusing cytosine conversion predominantly at position −18, this synergistic complex ensures highly precise targeting. Demonstrating enhanced efficiency across diverse models, including yeast and rice, the platform offers a robust solution for ...
Xi Luo, Yuyao Qu, Zhengyan Ye, Zheng Li, Yuanyan Zhang, Longjiong Luo, Shaokang Li, Wei Zhao, Ming Wang, Ralph Bock, Jianmin Wan, Junjie Tan   +11 more
wiley   +1 more source

Characteristics of the tree shrew gut virome.

PLoS ONE, 2019
The tree shrew (Tupaia belangeri) has been proposed as an alternative laboratory animal to primates in biomedical research in recent years. However, characteristics of the tree shrew gut virome remain unclear.
Linxia Chen, Wenpeng Gu, Chenxiu Liu, Wenguang Wang, Na Li, Yang Chen, Caixia Lu, Xiaomei Sun, Yuanyuan Han, Dexuan Kuang, Pinfen Tong, Jiejie Dai   +11 more
doaj   +1 more source

Analysis of a set of Australian northern brown bandicoot expressed sequence tags with comparison to the genome sequence of the South American grey short tailed opossum

BMC Genomics, 2007
Background Expressed sequence tags (ESTs) have been used for rapid gene discovery in a variety of organisms and provide a valuable resource for whole genome annotation. Although the genome of one marsupial, the opossum Monodelphis domestica, has now been
Miller Robert D, Lindblad-Toh Kerstin, Rosenberg George H, Nyberg April M, Indiviglio Sandra, Baker Michelle L, Papenfuss Anthony T   +6 more
doaj   +1 more source

Usability of the kink parameters for nucleic acid structure in Database [PDF]

Nucleic Acids Symposium Series, 2005
DNA/RNA molecules with the specific three-dimensional structure, express the specific structural and biological functions. The knowledge integration based on three dimensional structures can be used to explain biochemical observations, to predict biological functions and to design drugs specific to a given complex system.
openaire   +2 more sources