Conversion of cDNA differential display results (DDRT-PCR) into quantitative transcription profiles [PDF]
BMC Genomics, 2005 Background Gene expression studies on non-model organisms require open-end strategies for transcription profiling. Gel-based analysis of cDNA fragments allows to detect alterations in gene expression for genes which have neither been sequenced yet nor ...
Koopmann Birger +3 more
doaj +11 more sources
A fast, efficient and high-throughput procedure involving laser microdissection and RT droplet digital PCR for tissue-specific expression profiling of rice roots [PDF]
BMC Molecular and Cell Biology, 2020 Background In rice, the cortex and outer tissues play a key role in submergence tolerance. The cortex differentiates into aerenchyma, which are air-containing cavities that allow the flow of oxygen from shoots to roots, whereas exodermis suberification ...
Thibault Mounier +6 more
doaj +3 more sources
Optimized Method for the Identification of Candidate Genes and Molecular Maker Development Related to Drought Tolerance in Oil Palm (Elaeis guineensis Jacq.) [PDF]
Plants, 2022 Drought is a major constraint in oil palm (Elaeis guineensis Jacq.) production. As oil palm breeding takes a long time, molecular markers of genes related to drought tolerance characteristics were developed for effective selection.
Sunya Nuanlaong +3 more
doaj +2 more sources
Cloning of putative ureG genes from Glomus intraradices and urease activities in tobacco arbuscular mycorrhizal roots Clonagem de genes ureG putativos de Glomus intraradices e atividades de urease em raízes micorrízicas arbusculares de tabaco [PDF]
Scientia Agricola, 2009 Even though the major benefit of arbuscular mycorrhizae is the increased uptake of phosphate from the soil solution and translocation to the plant, changes in the activity of enzymes involved in nitrogen (N) metabolism have been detected in mycorrhizal ...
Daniele Takahashi +2 more
doaj +5 more sources
In vitro fitness of SARS-CoV-2 variants as assessed by competition experiments followed by ddRT-PCR and whole genome sequencing. [PDF]
J Clin Virol, 2023 To develop a new method for reliable and rapid determination of the fitness of SARS-CoV-2 variants of concern.Competition experiments between two SARS-CoV-2 variants were performed in cells of the upper (nasal human airway epithelium) and lower (Calu-3 cells) respiratory tracts followed by quantification of variant ratios by droplet digital reverse ...
Hénaut M +4 more
europepmc +3 more sources
Application of a ddRT-PCR to quantify seasonal influenza virus for viral isolation
Biosafety and Health, 2022 Viral isolation in cell cultures has been regarded for decades as the “gold standard” for the laboratory diagnosis of influenza viral infections. Not all viral strains could be isolated from clinical samples.
Yimeng Liu +11 more
doaj +2 more sources
Identification of Salt-Stress-Induced Genes from the RNA-Seq Data of Reaumuria trigyna Using Differential-Display Reverse Transcription PCR [PDF]
International Journal of Genomics, 2014 Next generation sequencing (NGS) technologies have been used to generate huge amounts of sequencing data from many organisms. However, the correct choice of candidate genes and prevention of false-positive results computed from digital gene expression ...
Zhen-hua Dang +5 more
doaj +4 more sources
DDRT-PCR approaches applied for preeminent results in the isolation of DETs from fish brain tissues [PDF]
Brazilian Journal of Biology, 2015 Differential Display (DD) is a technique widely used in studies of differential expression. Most of these analyses, especially those involving fish species, are restricted to species from North America and Europe or to commercial species, as salmonids ...
FA. Alves-Costa, AP. Wasko
doaj +6 more sources
West Nile Virus (Orthoflavivirus nilense) RNA concentrations in wastewater solids at five wastewater treatment plants in the United States [PDF]
PeerJBackground Orthoflavivirus nilense, formerly known as West Nile Virus (WNV), has become endemic to the United States since its introduction in 1999.
Alessandro Zulli +6 more
doaj +3 more sources
Identification and biological characterization of pathogen causing sooty blotch of Ardisia crispa (Thunb.) A.DC. [PDF]
PeerJSooty blotch, a primary leaf disease affecting Ardisia crispa (Thunb.) A.DC. (A. crispa, AC), significantly impacts both the yield and quality of this medicinal plant.
Demei Yang +5 more
doaj +3 more sources