Results 151 to 160 of about 1,153 (176)

Identification and isolation by DDRT-PCR of genes differentially expressed byHistoplasma capsulatumduring macrophages infection

Microbial Pathogenesis, 1998
Establishment of infection and disease implies modifications in the genetic programmes of the cell systems that are involved and the differential expression of genes in both parasite and host. In order to identify and isolate relevant genes of the fungus, Histoplasma capsulatum, in which expression is specifically induced during its interaction with ...
S, Colonna-Romano, A, Porta, A, Franco, G S, Kobayashi, B, Maresca   +4 more
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Specific maternal transcripts in bovine oocytes and cleavaged embryos: Identification with novel DDRT‐PCR methods

Molecular Reproduction and Development, 2005
AbstractWe used annealing control primer (ACP)‐based differential display reverse transcription polymerase chain reaction (DDRT‐PCR) to isolate differentially expressed amplicons in bovine germinal vesicle (GV) stage oocytes, 8‐cell stage embryos produced in vitro, and blastocyst stage embryos produced in vitro.
Kyu-Chan, Hwang, Sae-Young, Park, Se-Pill, Park, Jin Ho, Lim, Xiang-Shun, Cui, Nam-Hyung, Kim   +5 more
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Molecular profiling of non-genotoxic hepatocarcinogenesis using differential display reverse transcription-polymerase chain reaction (ddRT-PCR)

European Journal of Drug Metabolism and Pharmacokinetics, 1997
The technique of differential display reverse transcription-polymerase chain reaction (ddRT-PCR) has been used to produce unique profiles of up-regulated and down-regulated gene expression in the liver of male Wistar rats following short term exposure to the non-genotoxic hepatocarcinogens, phenobarbital and WY-14,643.
J C, Rockett, D J, Esdaile, G G, Gibson
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Detecting herbivore-specific transcriptional responses in plants with multiple DDRT-PCR and subtractive library procedures

Physiologia Plantarum, 2003
Differential display-reverse transcriptase PCR (DDRT-PCR) and subtractive hybridization with magnetic beads (SHMB) procedures were modified to compare the transcriptional responses of the postfire desert annual, Nicotiana attenuata when it is attacked ...
Voelckel, C., Baldwin, I.
openaire   +3 more sources

Investigation of Ochratoxin A biosynthetic genes inPenicillium verrucosum by DDRT-PCR experiments: differential expression of OTA genes

Mycotoxin Research, 2001
A defined minimal medium has been developed which is either restrictive or permissive for the production of ochratoxin A byPenicillium verrucosum simply by changing the nitrogen and carbon source. The combination of ammonium/glycerin promoted, whereas the combination nitrate/glucose repressed ochratoxin production.In parallel mutants ofP verrucosum ...
P, Färber, R, Geisen
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Transcriptional profiling by DDRT-PCR analysis reveals gene expression during seed development in Carya cathayensis Sarg.

Plant Physiology and Biochemistry, 2015
Hickory (Carya cathayensis Sarg.) seed has one of the highest oil content and is rich in polyunsaturated fatty acids (PUFAs), which kernel is helpful to human health, particularly to human brain function. A better elucidation of lipid accumulation mechanism would help to improve hickory production and seed quality. DDRT-PCR analysis was used to examine
You-Jun, Huang, Qin, Zhou, Jian-Qin, Huang, Yan-Ru, Zeng, Zheng-Jia, Wang, Qi-Xiang, Zhang, Yi-Hang, Zhu, Chen, Shen, Bing-Song, Zheng   +8 more
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[Optimizing the reaction system of DDRT-PCR by positive-cross test].

Yi chuan = Hereditas, 2008
The method of mRNA Differential display PCR (DDRT-PCR) is used to identify differentially expressed genes widely. To improve further the efficiency and reproducibility of the method, the positive-cross test were performed to find the optional conditions of DDRT-PCR by analyzing the six critical parameters of dNTPs, MgCl2, arbitrary primer, anchor ...
Shu-Fang, Liu, Li-Xin, Du, Jing, Zhu, Ai-Hua, Wang, Hong-Bin, Li   +4 more
openaire   +1 more source

Monitoring of Ochratoxin A biosynthesis related genes in Penicillium verrucosum by differential display reverse transcription PCR (DDRT-PCR) experiments.

Mycotoxin Research, 2002
Differential display reverse transcription polymerase chain reaction experiments were performed to monitore Ochratoxin A biosynthetic genes inPenicillium verrucosum BFE487. A stringent prerequisite for the performance of this type of experiments is the knowledge of the differential expression of OTA genes.
P, Färber, R, Geisen
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Untersuchungen zur Reproduzierbarkeit einer nichtradioaktiven DDRT-PCR-Methode zum Nachweis der Genexpression in Plattenepithelkarzinomzellen der oberen Luft-und Speisewege

Laryngo-Rhino-Otologie, 1997
BACKGROUND The analysis of the gene spectrum in tumor and normal cells may provide information about genes involved in the differentiation or in the genesis of tumors. Differential Display Reverse Transcriptase Polymerase Chain Reaction (DDRT-PCR) is an innovative method that enables quick analysis of almost all mRNA molecules expressed in the cells ...
T. Görögh, B. J. Folz, B. M. Lippert, S. Gottschlich, J. Externbrink, A. M. Niemann, J. A. Werner   +6 more
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A Clamp-Based One-Step Droplet Digital Reverse Transcription PCR (ddRT-PCR) for Precise Quantitation of Messenger RNA Mutation in Single Cells

ACS Sensors, 2018
Precise detection of the low copy numbers of messenger RNA (mRNA) mutation in single cells is of great significance but still remains challenging. Herein, by integrating the outstanding features of a rationally designed peptide nucleic acid (PNA) clamp for highly selective discrimination of single-nucleotide variation, and droplet digital PCR for ...
Yuanyuan Sun, Hui Tian, Chenghui Liu, Dandan Yang, Zhengping Li   +4 more
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