SARS-CoV-2 Viral Load and Cytokine Dynamics Profile as Early Signatures of Long COVID Condition in Hospitalized Individuals. [PDF]
Influenza Other Respir VirusesABSTRACT Background The global pandemic caused by SARS‐CoV‐2 has resulted in millions of people experiencing long COVID condition, a range of persistent symptoms following the acute phase, with an estimated prevalence of 27%–64%. Materials and Methods To understand its pathophysiology, we conducted a longitudinal study on viral load and cytokine ...
Alonso Domínguez J +9 more
europepmc +2 more sources
Cloning Method for Stress‐Resistant Gene of Conringia planisiliqua under Drought Stress
Journal of Healthcare Engineering, Volume 2021, Issue 1, 2021., 2021 The low temperature, drought, high salt, and other environments influence crop production and development directly, so the gene cloning method has become an effective biological means. In order to effectively improve the cloning effect, a gene cloning method for Conringia planisiliqua based on mRNA differential display technology was proposed. Based on
Yanfei Zhu +3 more
wiley +1 more source
Use of Two Reverse Transcriptases Eliminates False-Positive Results in Differential Display
BioTechniques, 1997 The selection of false-positive clones in differential display PCR (DDRT-PCR) represents a formidable drawback to this otherwise powerful technique of detecting subtle differences between cell types.
Ying-Ju Sung, Robert B. Denman
doaj +1 more source
Improved Screening of cDNAs Generated by mRNA Differential Display Enables the Selection of True Positives and the Isolation of Weakly Expressed Messages [PDF]
, 1997 The high percentage of false positives generated by differential display (as high as 85%) has previously limited the potential of the method. This report describes an efficient methodology that enables false positives to be discarded prior to cloning ...
Bouquin, Thomas +6 more
core +2 more sources
Emerging Microbes and Infections, 2023 Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been detected in wastewater. Wastewater-based epidemiology (WBE) is a practical and cost-effective tool for the assessment and controlling of pandemics and probably for examining SARS-CoV-2
Parashuram Kallem +4 more
doaj +1 more source
Analysis of Differential Display RT-PCR Products Using Fluorescent Primers and GENESCANTM Software
BioTechniques, 1997 Differential display reverse transcription PCR (DDRT-PCR) is a procedure used to identify the induction or repression of gene expression. In most DDRT-PCR protocols, radioisotopes are incorporated during PCR and the cDNA products are detected by ...
Kenneth R. Luehrsen +3 more
doaj +1 more source
Genetic, molecular and cellular approaches to the analysis of maize embryo development [PDF]
, 2009 The development of embryo structures in plants is essential for the formation of the adult plant organs. In cereals, this process has distinct features which have attracted attention from different points of view.
Bastida i Virgili, Miriam +7 more
core +3 more sources
Die S19 mRNS-Expression in Plattenepithelkarzinomen des oberen Aerodigestivtraktes [PDF]
, 2005 Ziel dieser Arbeit war die Analyse von Ausmaß, Häufigkeit und Charakter der mRNS-Expression des ribosomalen Proteins S19 (S19) in Head and Neck Squamous Cell Carcinoma (HNSCC).
Sengpiel, Verena +1 more
core +1 more source
Molecular cloning and characterization of Polygalacturonase-Inhibiting Protein and Cinnamoyl-Coa Reductase genes and their association with fruit storage conditions in blueberry (Vaccinium corymbosum) [PDF]
, 1997 Blueberry is a widely grown and easily perishable fruit crop. An efficient post-harvest handling is critical, and for that purpose gene technology methods have been part of ongoing programmes to improve crops with high food values such as blueberry. Here
BleekerWagemakers, EM +7 more
core +2 more sources
Mısır-mısır pası uyumlu interaksiyonunda ekspresyonu değişim gösteren genlerin DDRT-PCR analizi
Mediterranean Agricultural Sciences, 2020 Bitki-mikrop interaksiyonlarında ekspresyonu değişim gösteren genlerin tanımlanması, konukçu direnci ve duyarlılığında gerçekleşen fizyolojik değişimler ve bunlardan sorumlu genetik faktörler ve mekanizmalar hakkında değerli bilgiler sağlamaktadır. Mısır (Zea mays) ve mısır pası (Puccinia sorghi) uyumlu interaksiyonunda gerçekleştirdiğimiz çalışmada ...
Hatice ÇİLKOL, Mehmet Ali SÜDÜPAK
openaire +3 more sources