Results 101 to 110 of about 334,258 (285)

Optimizing de novo common wheat transcriptome assembly using short-read RNA-Seq data

BMC Genomics, 2012
Background Rapid advances in next-generation sequencing methods have provided new opportunities for transcriptome sequencing (RNA-Seq). The unprecedented sequencing depth provided by RNA-Seq makes it a powerful and cost-efficient method for transcriptome
Duan Jialei, Xia Chuan, Zhao Guangyao, Jia Jizeng, Kong Xiuying   +4 more
doaj   +1 more source

The effect of variant interference on de novo assembly for viral deep sequencing

BMC Genomics, 2020
Background Viruses have high mutation rates and generally exist as a mixture of variants in biological samples. Next-generation sequencing (NGS) approaches have surpassed Sanger for generating long viral sequences, yet how variants affect NGS de novo ...
Christina J. Castro, Rachel L. Marine, Edward Ramos, Terry Fei Fan Ng   +3 more
doaj   +1 more source

Cell Adhesion by Design: Engineering Tissue Culture Scaffolds With Adhesion Cues

Advanced Materials Interfaces, EarlyView.
ABSTRACT In scaffold‐based tissue engineering, the matrix should provide adequate adhesion cues for cell attachment, spreading, and function. Given the multitude of adhesion receptors and the diversity of scaffolds, there are many approaches to render scaffolds adhesive, even though they are not all equivalent.
Dalia Dranseike, Dhananjay V. Deshmukh, Mark W. Tibbitt, Céline Labouesse   +3 more
wiley   +1 more source

Combining independent de novo assemblies to optimize leaf transcriptome of Persian walnut.

PLoS ONE, 2020
Transcriptome resources can facilitate to increase yield and quality of walnuts. Finding the best transcriptome assembly has not been the subject of walnuts research as yet.
Mohammad Sadat-Hosseini, Mohammad Reza Bakhtiarizadeh, Naser Boroomand, Masoud Tohidfar, Kourosh Vahdati   +4 more
doaj   +1 more source

Haplotype-resolved de novo assembly with phased assembly graphs

, 2020
Haplotype-resolved de novo assembly is the ultimate solution to the study of sequence variations in a genome. However, existing algorithms either collapse heterozygous alleles into one consensus copy or fail to cleanly separate the haplotypes to produce high-quality phased assemblies.
Cheng, Haoyu, Concepcion, Gregory T, Feng, Xiaowen, Zhang, Haowen, Li, Heng   +4 more
openaire   +2 more sources

RNF213 Is an Interferon‐Stimulated Gene That Targets Influenza A Virus NP and Activates MDA5 to Restrict Infection

Advanced Science, EarlyView.
RNF213 is characterized as a dual‐functional antiviral effector. It directly mediates the degradation of the influenza A virus nucleoprotein (NP) while simultaneously activating the MDA5‐mediated innate immune signaling pathway. This coordinated response establishes a powerful host defense system against viral infection. ABSTRACT Influenza A virus (IAV)
Haoning Li, Yuying Zhang, Jingjing Wang, Qianqian Zhang, Hailiang Sun, Fanhua Wei   +5 more
wiley   +1 more source

Transcriptome analysis identifies metallothionein as biomarkers to predict recurrence in hepatocellular cacinoma

Molecular Genetics & Genomic Medicine, 2019
Background Liver cancer is the fifth most common cancer, and hepatocellular carcinoma (HCC) is the major liver tumor type seen in adults. HCC is usually caused by chronic liver disease such as hepatitis B virus or hepatitis C virus infection.
Sufang Wang, Michael Gribskov
doaj   +1 more source

Cellular Snowballing: Cell Adhesion and Migration Drive the Self‐Assembly of Cell‐Microgel Biohybrid Spheroids

Advanced Science, EarlyView.
A new class of biohybrid spheroids is engineered through the self‐assembly of adherent cells and extracellular matrix‐mimetic hydrogel microparticles (microgels). By mimicking a snowballing effect, this approach enables scalable formation of porous, millimeter‐scale spheroids with enhanced cell viability and molecular diffusion.
Zaman Ataie, Sina Kheirabadi, Changhao Li, Aneesh Risbud, Aswathy Sebastian, Istvan Albert, Sulin Zhang, Amir Sheikhi   +7 more
wiley   +1 more source

De novo transcriptome assembly workflow v1

, 2016
This protocol describesthe production ofa reference-qualityde novotranscriptome assembly for the spiny mouse (Acomys cahirinus).These methods can be applied to other RNA-Seq datasets to generate high-quality transcriptome assemblies in other species.Validation and description of assembly output is described in 'De novo transcriptome assembly for the ...
Jared Mamrot, not provided Roxane Legaie, not provided Stacey J Ellery, not provided Trevor Wilson, not provided Torsten Seemann, not provided David Gardner, not provided David W Walker, not provided Peter Temple-Smith, not provided Anthony T Papenfuss, not provided Hayley Dickinson   +9 more
openaire   +1 more source

Telomere‐to‐Telomere Genomes Reveal that Multiscale Evolution Shapes the Largest Metabolic Arsenal of Diaporthe Fungi

Advanced Science, EarlyView.
This study presents the first telomere‐to‐telomere genomes and population resources for Diaporthe pathogens, uncovering the largest known fungal repertoire of secondary metabolite gene clusters. Structural variations and horizontal gene transfer drive cluster diversification, while specific rapidly evolving clusters control virulence, offering novel ...
Kainan Li, Chen Zhang, Zhichao Zhang, Jiaheng Cheng, Qiuyan Gao, Long Gao, Xiaolin Zhao, Wei Yan, Yuanchao Wang, Wenwu Ye   +9 more
wiley   +1 more source