Results 111 to 120 of about 334,258 (285)

Mass Spectrometry Imaging‐Assisted Discovery of Gallotannin Biosynthetic Genes in the Root of Paeonia suffruticosa

Advanced Science, EarlyView.
An integrated approach combining mass spectrometry imaging, transcriptomics, and phylogenetic analysis facilitated the efficient identification of genes involved in gallotannin biosynthesis in Paeonia suffruticosa. As a result, a key UGT and several SCPL acyltransferases responsible for biosynthesizing pentagalloylglucose were successfully identified ...
Yushi Liu, Wenna Duan, Weiwei Tang, Yucheng Zhao, Bin Li   +4 more
wiley   +1 more source

Hybrid de novo genome assembly and centromere characterization of the gray mouse lemur (Microcebus murinus)

BMC Biology, 2017
Background The de novo assembly of repeat-rich mammalian genomes using only high-throughput short read sequencing data typically results in highly fragmented genome assemblies that limit downstream applications.
Peter A. Larsen, R. Alan Harris, Yue Liu, Shwetha C. Murali, C. Ryan Campbell, Adam D. Brown, Beth A. Sullivan, Jennifer Shelton, Susan J. Brown, Muthuswamy Raveendran, Olga Dudchenko, Ido Machol, Neva C. Durand, Muhammad S. Shamim, Erez Lieberman Aiden, Donna M. Muzny, Richard A. Gibbs, Anne D. Yoder, Jeffrey Rogers, Kim C. Worley   +19 more
doaj   +1 more source

De novo Nanopore read quality improvement using deep learning. [PDF]

, 2019
BACKGROUND:Long read sequencing technologies such as Oxford Nanopore can greatly decrease the complexity of de novo genome assembly and large structural variation identification.
Egan, Rob, LaPierre, Nathan, Wang, Wei, Wang, Zhong   +3 more
core  

De Novo Design and Directed Evolution Refinement of Mirror‐Image Protein Binders Targeting Interleukin‐4

Advanced Science, EarlyView.
This study presents the de novo design and directed evolution of a mirror‐image D‐protein inhibitor targeting human interleukin‐4 (IL‐4). The engineered molecule exhibits nanomolar binding affinity for IL‐4 and effectively inhibits IL‐4–mediated signaling.
Liqing Xu, Yuxiang Ren, Tongyue Wang, Hongyong Li, Yue Yao, Lei Liu, Ke Sun, Peilong Lu   +7 more
wiley   +1 more source

De novo transcriptome assembly of shrimp Palaemon serratus

Genomics Data, 2017
The shrimp Palaemon serratus is a coastal decapod crustacean with a high commercial value. It is harvested for human consumption. In this study, we used Illumina sequencing technology (HiSeq 2000) to sequence, assemble and annotate the transcriptome of P. serratus. RNA was isolated from muscle of adults individuals and, from a pool of larvae.
Alejandra Perina, Ana M González-Tizón, Iago F. Meilán, Andrés Martínez-Lage   +3 more
openaire   +3 more sources

Plant assemble - Plant de novo genome assembly: scaffolding v2

, 2022
With the advancement of long-read sequencing technologies and associated bioinformatics tools, it has now become possible to de novo assemble complex plant genomes with unrivalled contiguity, completeness and correctness. As read lengths can surpass repeat lengths, the ability to assemble genomes de novo has dramatically improved, whereby complex plant
Scott Ferguson, Ashley Jones, Justin Borevitz   +2 more
openaire   +1 more source

Hierarchical Targeting of TREM2+ Myeloid Cells via Acid‐Triggered OMVs Reprogram Immunosuppression and Suppress Osteolysis in Bone‐Metastatic TNBC

Advanced Science, EarlyView.
This study developed a hierarchical targeting nanoplatform, siTREM2@ETP‐PEOz‐OMVs, against triple‐negative breast cancer (TNBC) bone metastasis. It precisely delivers therapeutic siTREM2 to monocytes/macrophages within the metastatic niche. This intervention dually regulates cell fate: reprogramming immunosuppressive macrophages and inhibiting ...
Fanglu Chen, Yucheng Xue, Xiao Ma, Liang Chen, Zhenxuan Shao, Hangxiang Sun, Haochen Mou, Fangqian Wang, Zilong Li, Yechao Shen, Zhaoming Ye, Xiaohua Yu, Shengdong Wang   +12 more
wiley   +1 more source

Autism and mild epilepsy associated with a de novo missense pathogenic variant in the GTPase effector domain of DNM1

American Journal of Medical Genetics Part C: Seminars in Medical Genetics, EarlyView., 2023
Abstract Dynamin 1 is a GTPase protein involved in synaptic vesicle fission, which facilitates the exocytosis of neurotransmitters necessary for normal signaling. Pathogenic variants in the DNM1 gene are associated with intractable epilepsy, often manifested as infantile spasms at onset, developmental delay, and a movement disorder, and are located in ...
Davide Mei, Elena Parrini, Claudia Bianchini, Maria Luisa Ricci, Renzo Guerrini   +4 more
wiley   +1 more source

RNA-Seq analysis and de novo transcriptome assembly of Coffea arabica and Coffea eugenioides : P0068 [PDF]

, 2013
Coffee is one of the most important agricultural commodites in the world. Coffea arabica, an allotetraploid from a recent hybridization of two diploid species (C.canephora and C.eugenioides) is responsible for 70% of world commercial production.
Carazzolle, Marcelo Falsarella, Charmetant, Pierre, Domingues, Douglas S., Ivamoto, Suzana Tiemi, Leroy, Thierry, Mishima, Jaime Hikaru, Protasio Pereira, Luiz Filipe, Reis Júnior, Osvaldo, Yuyama, Priscilla Mary   +8 more
core  

Plant assemble - Plant de novo genome assembly: annotation v2

, 2022
With the advancement of long-read sequencing technologies and associated bioinformatics tools, it has now become possible to de novo assemble complex plant genomes with unrivalled contiguity, completeness and correctness. As read lengths can surpass repeat lengths, the ability to assemble genomes de novo has dramatically improved, whereby complex plant
Scott Ferguson, Ashley Jones, Justin Borevitz   +2 more
openaire   +1 more source