Results 191 to 200 of about 32,605 (213)
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Fractionation of nucleic acid on benzoylated-naphthoylated DEAE cellulose
Journal of Molecular Biology, 1967Nucleic acids have been fractionated by column chromatography using methylated albumin kieselguhr (Mandell & Hershey, 1960; Sueoka & Cheng, 1962) cellulose CF-11 (Franklin, 1966), agarose (Erikson & Gordon, 1966) and hydroxyapatite (Muench & Berg, 1966).
Sedat, John W. +2 more
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Immobilisierung von polymergebundenen oligodesoxyribocytidylsäuren an DEAE‐cellulose
Die Makromolekulare Chemie, 1981AbstractN‐Anisoyl‐deoxyribocytidine‐5′‐phosphate is chemically polycondensed. From the polycondensate, oligomers containing four or more monomeric units are isolated chromatographically and subsequently linked to a soluble polyvinylalcohol. After cleavage of the nucleobaseprotecting groups, the unreacted oligodesoxyribocytidylic acids are separated ...
Herbert Schott, Hildegard Watzlawick
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Isolation of IgG by DEAE Cellulose
1979Weakly basic cellulose ion exchanges, such as DEAE cellulose, can be used for the separation of serum proteins according to the procedure of Sober and co-workers (1956). This procedure is especially useful for isolation of IgG antibodies, which leave the column in a relatively pure form right after the void volume.
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Decontamination of polymerase chain reaction reagents using DEAE–cellulose
Analytical Biochemistry, 2009Polymerase chain reaction (PCR) is widely used to detect specific DNA sequences for purposes of microbial identification, clinical diagnosis, and basic research. The most pernicious problem plaguing this technique is contamination of PCR reagents with previously amplified material.
S A, Glushkov +2 more
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Separation of Heparin into Subtractions by DEAE-Cellulose Chromatography
Thrombosis and Haemostasis, 1979SummaryCommercial porcine heparin can be separated into three distinct subtractions by using DEAE-cellulose chromatography and a stepped salt gradient. Gram quantities of heparin can be fractionated by this technique. All three heparin subtractions can accelerate the inhibition of thrombin by antithrombin III with different efficiency.
R H, Yue, T, Starr, M M, Gertler
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Fractionation of Calf Thymus DNA by DEAE Cellulose Column Chromatography
Preparative Biochemistry, 1982Commercially available calf thymus DNA could be fractionated into different populations by DEAE-cellulose column chromatography, presumably due to the tertiary folding of the solid fibrous form of DNA which opened up gradually in solution. Depending on the duration in solution or the concentration of the solution, various peaks could be obtained by ...
A F, Wu, T T, Wu
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Two forms of striatal tyrosine hydroxylase from DEAE-cellulose chromatography
Brain Research, 1983Tyrosine hydroxylase (TH) in freshly prepared 45,000 g supernatant from rat striatum was fractionated by DEAE-cellulose chromatography. The elution was made with 2 vols. of buffer (50 mM Tris, pH 7.4; 2 mM dithiothreitol) followed by 4 vols. of a linear NaCl gradient (0 0.3 M) in the same buffer.
P Y, Sze, R F, Alderson, B J, Hedrick
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Batch preparation of rabbit γG globulin with DEAE-cellulose
Molecular Immunology, 1967Abstract A rapid method for the preparatoin of γG globulin of specified purity from rabbit serum by a single batch absorption with DEAE-cellulose is described. The percentage purity of the product depends upon the quantity of DEAE-cellulose used per ml serum.
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Separation of adenovirus by chromatography on DEAE-cellulose
Virology, 1961I, HARUNA, H, YAOI, R, KONO, I, WATANABE
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