Results 61 to 70 of about 18,296 (185)

Proposed role of Bcl-x_L deamidation and methylation in apoptosis.

, 2013
Deamidation-isomerization of two critical Asn residues of Bcl-xL abolishes its antiapoptotic function. Methylation of the same residues can restore the functional integrity of Bcl-xL through repair of the isopeptide bonds.
Amelia Cimmino (188570), Vincenzo Zappia (376623), Marianna Raimo (376619), Fabbri Muller (376616), Diego Ingrosso (333700), Stefania D'Angelo (376621), Rosanna Capasso (333692), Maria Luigia De Bonis (126770), Irene Sambri (333693), Patrizia Galletti (376624), Lucia Masella (376617)   +10 more
core   +1 more source

From Enrichment to Insight: Leveraging Preparative cIEF Enrichments and Characterization to Support Data‐Driven Integration Practices for cIEF‐based Analytics

ELECTROPHORESIS, EarlyView.
Preparative cIEF enrichment enabled isolation of a low‐abundance, poorly resolved basic charge variant identified during routine cIEF analysis. Orthogonal characterization by peptide mapping and SCX‐UV–MS revealed incomplete N‐terminal pyroglutamate formation and Fc methionine oxidation.
Ramya Rao, Dominik Herrmann, Seamus Kelley, Victoria Cotham, Logan Figular, Youmi Moon, Deanna Di Grandi, Kathir Muthusamy, Yue Fu, Nisha Palackal, Shunhai Wang, Ning Li, Jennifer B. Nguyen, Michael P. Rosconi, Erica A. Pyles   +14 more
wiley   +1 more source

Western blots of in vitro deamidation assays of McpAc show CheD-mediated receptor modifications.

, 2013
Deamidation of the receptor fragment results in higher-migrating bands on a polyacrylamide gel. CheC is added, as indicated, and inhibits deamidation. Lanes 2 and 3 show McpAc incubated with wild-type GST-CheD, and lanes 4–11 show McpAc incubated with ...
Christopher V. Rao (244863), George D. Glekas (296002), Wei Yuan (64653), Hanna E. Walukiewicz (296007), George W. Ordal (296008), George M. Allen (296005)   +5 more
core   +1 more source

Chemical and conformational changes in proteins through clean label strategies for enhanced functionality: Recent advances

Food Biomacromolecules, EarlyView.
Abstract Process‐based functionalization of food materials has gained immense interest as they contribute to the development of clean label ingredients that can offer similar functional properties as food additives of synthetic origin. Chemical and conformational changes in proteins can induce diverse and targeted functionalities that can be useful as ...
Bindvi Arora, Upasana Yadav, Lekshmi SG, Alka Joshi, Pallavi Singh, Anamika Thakur, Shruti Sethi, Dinesh Kumar   +7 more
wiley   +1 more source

Top-down study of β2-microglobulin deamidation

, 2012
Although differentiation of the isomeric Asn deamidation products (Asp and isoAsp) at the peptide level by electron capture dissociation (ECD) has been well-established, isoAsp identification at the intact protein level remains a challenging task.
Yu, Xiang, O’Connor, Peter B., Li, Xiaojuan, Lin, Cheng, Costello, Catherine E.   +4 more
core   +1 more source

Proteomic and Metabolomic Profiling Reveal Mitochondrial Transplantation–Mediated Reprogramming in Gastric Cancer Cells

The Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Mitochondria provide multiple functions for cellular physiology. Transplantation of mitochondria isolated from gastric epithelial cells GES‐1 reducing the malignancy of gastric cancer cells AGS was previously reported. To elucidate the underlying mechanisms, TMT‐based proteomic analysis coupling ingenuity pathway software prediction revealed ...
Ping‐Chen Chen, Chen‐Kai Liu, Ching‐Chung Tsai, Erna Sulistyowati, Fu‐An Li, Chung‐Jung Liu, Deng‐Chyang Wu, Shih‐Hsuan Chou, Fu‐Chen Kuo, Bin Huang   +9 more
wiley   +1 more source

Purified protein glutaminase from Chryseobacterium proteolyticum enhances the properties of wheat gluten

Food Chemistry: X
Protein glutaminase (PG), originating from Chryseobacterium proteolyticum, can catalyze the deamidation of glutamine residues in plant proteins into glutamic acid, thus enhancing its functional properties.
Zheng Zhang, Rui Shi, Xiaoyu Zhu, Lihui Zheng, Mingfei Jin, Deming Jiang, Yelin Wu, Hongliang Gao, Zhongyi Chang, Dongrui Wang, Jiajing Wu, Jing Huang   +11 more
doaj   +1 more source

Selective Deamidation and Enzymatic Methylation of Seminal Ribonuclease [PDF]

, 1986
Isoenzymatic forms alfa2, alfa-beta, and beta2 of bovine seminal ribonuclease are generated by the transformation of beta-type into alfa-type subunit through deamidation of a single amide group. The residue involved in this selective deamidation has been
D'ALESSIO, GIUSEPPE, DI DONATO, ALBERTO, Galletti P.   +2 more
core  

Deamidation Can Compromise Antibody Colloidal Stability and Enhance Aggregation in a pH-Dependent Manner

, 2019
Monoclonal antibodies must be both chemically and physically stable to be developed into safe and effective drugs. Although there has been considerable progress in separately understanding the molecular determinants of antibody chemical and physical ...
Thomas R. Slaney (1969774), Gregory V. Barnett, Tapan K. Das (4591117), Tapan K. Das, Gregory V. Barnett (2264980), Magfur E. Alam (3120765), Charles G. Starr (6452426), Peter M. Tessier (1546465), Thomas R. Slaney, Peter M. Tessier, Magfur E. Alam, Charles G. Starr   +11 more
core   +1 more source

Ion Activation Methods for Top‐Down Proteomics

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley   +1 more source