Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity
npj Vaccines, 2023 Flagellin, the TLR5 agonist, shows potent adjuvant activities in diverse vaccines and immunotherapies. Vibrio vulnificus flagellin B expressed in eukaryotic cells (eFlaB) could not stimulate TLR5 signaling. Enzymatic deglycosylation restored eFlaB’s TLR5
Koemchhoy Khim +7 more
doaj +4 more sources
From glycosylation to deglycosylation: Unraveling the complete deglycosylation processing pathway of rhodopsin [PDF]
Science ProgressN-linked glycosylation is a prevalent post-translational modification during protein maturation, which plays a pivotal role in protein folding, stability, localization, and oligomerization.
Xuan Chen +9 more
doaj +3 more sources
The N-glycan Glycoprotein Deglycosylation Complex (Gpd) from Capnocytophaga canimorsus Deglycosylates Human IgG [PDF]
PLoS Pathogens, 2011 C. canimorsus 5 has the capacity to grow at the expenses of glycan moieties from host cells N-glycoproteins. Here, we show that C. canimorsus 5 also has the capacity to deglycosylate human IgG and we analyze the deglycosylation mechanism. We show that deglycosylation is achieved by a large complex spanning the outer membrane and consisting of the Gpd ...
Renzi, Francesco +5 more
openaire +8 more sources
Enzymatic Deglycosylation of Glycoproteins
, 2013 Recombinant protein expression using eukaryotic expression systems has certain advantages, such as addition of posttranslational modifications that help protein stability and activity. Asparagine-linked sugar attachment is one of the most common posttranslation modifications.
Kim, MS, Leahy, D
openaire +4 more sources
Toward Personalized Theranostics: A Modular PET Platform for Antibody‐Based Imaging and Therapy [PDF]
Advanced Science, EarlyView.This modular, site‐specific antibody platform enables customizable conjugates for PET imaging and therapy. Interchangeable payloads – including radiometals, cytotoxics, and fluorophores – support adaptable, multimodal applications with limited off‐target effects. In vivo, the DFO/DOTAGA bischelate immunoconjugate provided clear tumor delineation in PET/
Delphine Vivier +16 more
wiley +2 more sources
IgG Glycosylation‐Dependent CLEC7A Signaling Drives Podocyte Dysfunction in Lupus Nephritis [PDF]
Arthritis &Rheumatology, EarlyView.Objective Lupus nephritis (LN) is a severe complication of systemic lupus erythematosus (SLE) that can lead to end‐stage kidney disease and increased mortality. IgG from patients with LN displays abnormal glycosylation, contributing to podocyte injury.
Rohit Upadhyay +3 more
wiley +2 more sources
Mechanism of antibody-specific deglycosylation and immune evasion by Streptococcal IgG-specific endoglycosidases [PDF]
Nature Communications, 2023 Bacterial pathogens have evolved intricate mechanisms to evade the human immune system, including the production of immunomodulatory enzymes. Here, the authors establish the mechanisms of recognition and specific deglycosylation of IgG antibodies by the ...
Beatriz Trastoy +16 more
doaj +2 more sources
Automated Enzymatic Deglycosylation of Monoclonal Antibody with the SampleStream Platform. [PDF]
J Am Soc Mass SpectromEngineered monoclonal antibodies have proven themselves as invaluable biotherapeutics used in clinics worldwide. In discovery, development, and production, it is essential that they are accurately validated to ensure their homogeneity, consistency ...
Fisher TD +5 more
europepmc +2 more sources
OGA is associated with deglycosylation of NONO and the KU complex during DNA damage repair
Cell Death and Disease, 2021 Accumulated evidence shows that OGT-mediated O-GlcNAcylation plays an important role in response to DNA damage repair. However, it is unclear if the “eraser” O-GlcNAcase (OGA) participates in this cellular process.
Yaqi Cui +8 more
doaj +2 more sources
N-linked glycosylation of NS1 protein modulates progeny virion assembly in orthoflaviviruses. [PDF]
PLoS PathogensTembusu virus (TMUV) is a mosquito-borne avian virus belonging to the genus Orthoflavivirus within the family Flaviviridae. The nonstructural protein 1 (NS1) of TMUV is a secretory protein containing three N-linked glycosylation sites at residues N130 ...
Senzhao Zhang +21 more
doaj +2 more sources