Protein Pulldown Assays to Monitor the Composition of the Bacterial RNA Degradosome
The method of co-immunoprecipitation (co-IP or pulldown) enables the identification of proteins interacting in macromolecular assemblies, through the purification of a key protein by affinity chromatography using specific antibodies immobilized on a matrix.
Steven W. Hardwick+2 more
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The Roles Of Rhle And Hfq In Srna-Dependent Gene Regulation [PDF]
THE ROLES OF RHLE AND HFQ IN SRNA-DEPENDENT GENE REGULATION by ABEYKOON JAYALATH IRESHA SANDEEPANIE RATHNAYAKE DECEMBER 2010 Advisor: Dr. Andrew Feig Major: Chemistry Degree: Master of Science Bacteria are adapted to live in diverse environmental ...
Rathnayake, Abeykoon Jayalath Iresha Sandeepanie
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The influence of microgravity on invasive growth in Saccharomyces cerevisiae [PDF]
This study investigates the effects of microgravity on colony growth and the morphological transition from single cells to short invasive filaments in the model eukaryotic organism Saccharomyces cerevisiae. Two-dimensional spreading of the yeast colonies
Daenen, Luk+8 more
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Cytoplasmic and Periplasmic Proteomic Signatures of Exponentially Growing Cells of the Psychrophilic Bacterium Pseudoalteromonas haloplanktis TAC125 [PDF]
peer ...
Albrecht, D.+11 more
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Degradation of RNA in bacteria: comparison of mRNA and stable RNA [PDF]
Degradation of RNA plays a central role in RNA metabolism. In recent years, our knowledge of the mechanisms of RNA degradation has increased considerably with discovery of the participating RNases and analysis of mutants affected in the various ...
Deutscher, Murray P.
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A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway [PDF]
The pluripotency factor Lin28 blocks the expression of let-7 microRNAs (miRNAs) in undifferentiated cells during development and functions as an oncogene in a subset of cancers1.
Chang, Hao-Ming+3 more
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Polyadenylation Promotes Degradation of 3′-Structured RNA by theEscherichia coli mRNA Degradosome in Vitro [PDF]
Erwin Blum+2 more
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Escherichia coli Poly(A)-binding Proteins That Interact with Components of Degradosomes or Impede RNA Decay Mediated by Polynucleotide Phosphorylase and RNase E [PDF]
Yanan Feng+3 more
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Characterization of Yersinia Pseudotuberculosis Abiotic Stress Responses [PDF]
Bacteria require optimal growth conditions in order to thrive. However, stress factors such as cold and oxidation can attribute to the pathogenecity of a species.
Henry, Amanda Joyce
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Physical and functional interactions among RNase E, polynucleotide phosphorylase and the cold‐shock protein, CsdA: evidence for a ‘cold shock degradosome’ [PDF]
Annie Prud’homme‐Généreux+5 more
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