Results 161 to 170 of about 2,784 (175)

Polyphosphate kinase is a component of the Escherichia coli RNA degradosome

Molecular Microbiology, 1997
The Escherichia coli degradosome is a multienzyme complex with four major protein components: the endoribonuclease RNase E, the exoribonuclease PNPase, the RNA helicase RhlB and enolase. The first three of these proteins are known to have important functions in mRNA processing and degradation.
E, Blum, B, Py, A J, Carpousis, C F, Higgins   +3 more
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Analysis of the RNA degradosome complex in Vibrio angustum S14

The FEBS Journal, 2010
The RNA degradosome is built on the C‐terminal half of ribonuclease E (RNase E) which shows high sequence variation, even amongst closely related species. This is intriguing given its central role in RNA processing and mRNA decay. Previously, we have identified RhlB (ATP‐dependent DEAD‐box RNA helicase)‐binding, PNPase (polynucleotide phosphorylase ...
Melissa A, Erce, Jason K K, Low, Marc R, Wilkins   +2 more
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Analysis of the Escherichia coli RNA degradosome composition by a proteomic approach

Biochimie, 2006
The RNA degradosome is a bacterial protein machine devoted to RNA degradation and processing. In Escherichia coli it is typically composed of the endoribonuclease RNase E, which also serves as a scaffold for the other components, the exoribonuclease PNPase, the RNA helicase RhlB, and enolase.
M.E. Regonesi, M. Del Favero, F. Basilico, F. Briani, L. Benazzi, P. Tortora, P. Mauri, G. Dehò   +7 more
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Preparation of Escherichia coli Rne Protein and Reconstitution of RNA Degradosome

2001
Publisher Summary This chapter describes methods for the purification of Rne, the key scaffold in the assembly process, and for the reconstitution of active degradosomes from individually purified enzymes. The endoribonuclease RNase E was initially described as an activity required for the penultimate step in the maturation of 5S rRNA, the processing
G A, Mackie, G A, Coburn, X, Miao, D J, Briant, A, Prud'homme-Genereux   +4 more
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The assembly and distribution in vivo of the Escherichia coli RNA degradosome

Biochimie, 2013
We report an analysis in vivo of the RNA degradosome assembly of Escherichia coli. Employing fluorescence microscopy imaging and fluorescence energy transfer (FRET) measurements, we present evidence for in vivo pairwise interactions between RNase E-PNPase (polynucleotide phosphorylase), and RNase E-Enolase.
Lilianha, Domínguez-Malfavón, León D, Islas, Ben F, Luisi, Refugio, García-Villegas, Jaime, García-Mena   +4 more
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The RNA degradosome: life in the fast lane of adaptive molecular evolution

Trends in Biochemical Sciences, 2006
In Escherichia coli, the multi-enzyme RNA degradosome contributes to the global, posttranscriptional regulation of gene expression. The degradosome components are recognized through natively unstructured "microdomains" comprising as few as 15-40 amino acids.
Maria Jose, Marcaida, Mark A, DePristo, Vidya, Chandran, Agamemnon J, Carpousis, Ben F, Luisi   +4 more
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Genetic and Biochemical Approaches for Analysis of Mitochondrial Degradosome from Saccharomyces cerevisiae

2001
Publisher Summary Regulation of RNA turnover is one of the major mechanisms of controlling gene expression in yeast mitochondria. RNA degradation is carried out by the yeast mitochondrial degradosome (also known as mtEXO); a multiprotein enzyme complex showing NTP-dependent 3′ → 5′-exoribonuclease activity in vitro.
A, Dziembowski, P P, Stepien
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Structural Studies of the Yeast Mitochondrial Degradosome

2005
The yeast mitochondrial degradosome/exosome (mtExo) is responsible for most RNA turnover in mitochondria and has been proposed to form a central part of a mitochondrial RNA surveillance system responsible for degradation of aberrant and unprocessed RNA ([1], [2]).
Feddersen, Ane, Jonstrup, Anette Thyssen, Brodersen, Ditlev Egeskov   +2 more
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Composition and adpatation of the E. coli RNA degradosome

2004
Although transcription and translation are recognized mechanisms for regulating gene expression, control over RNA stability can also accomplish this task. In E. coli, bulk mRNA degradation is largely carried out by a complex of enzymes called the degradosome. It is composed of the endonuclease RNase E, the exonuclease PNPase, the helicase RhIB, and the
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Towards Understanding Helicase and Chaperone Activities in the RNA Degradosome

2020
The E. coli RNA degradosome is a complex multi-enzyme machine which is central to the post-transcriptional regulation of the cell. Some of its functions include maturing and processing sRNA, rRNA, and tRNA, as well as degrading mRNA. The key components of the RNA degradosome include the endoribonuclease RNase E, the DEAD-box RNA helicase RhlB, the ...
openaire   +1 more source