Results 171 to 180 of about 4,051 (196)
Reconstitution and Analysis of the Multienzyme Escherichia coli RNA Degradosome
Journal of Molecular Biology, 2008 The Escherichia coli RNA degradosome is a multienzyme assembly that functions in transcript turnover and maturation of structured RNA precursors. We have developed a procedure to reconstitute the RNA degradosome from recombinant components using modular coexpression vectors.
Jonathan A R Worrall +2 more
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The RNA degradosome promotes tRNA quality control through clearance of hypomodified tRNA [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2019 The factors and mechanisms that govern tRNA stability in bacteria are not well understood. Here, we investigated the influence of posttranscriptional modification of bacterial tRNAs (tRNA modification) on tRNA stability. We focused on ThiI-generated 4-thiouridine (s 4 U), a modification found in bacterial and ...
Satoshi Kimura, Matthew K Waldor
exaly +3 more sources
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Current perspectives of the Escherichia coli RNA degradosome
Biotechnology Letters, 2011Many biological processes in the cell are linked to RNA metabolism and therefore have implications for a wide range of biotechnological applications. The processing and degradation of RNA plays an important role in RNA metabolism with often the same enzymes being involved in both processes.
Adélle, Burger +2 more
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Escherichia coli RNA Degradosome
2001Publisher Summary The Escherichia coli RNA degradosome is a nucleolytic complex containing four integral components: ribonuclease E (RNase E), polynucleotide phosphorylase (PNPase), RNA helicase B (RhlB), and enolase. This complex was discovered during efforts to purify and characterize RNase E, which is a singlestrand-specific endoribonuclease ...
A J, Carpousis +3 more
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The RNA degradosome: life in the fast lane of adaptive molecular evolution
Trends in Biochemical Sciences, 2006In Escherichia coli, the multi-enzyme RNA degradosome contributes to the global, posttranscriptional regulation of gene expression. The degradosome components are recognized through natively unstructured "microdomains" comprising as few as 15-40 amino acids.
Maria J Marcaida +2 more
exaly +3 more sources
The assembly and distribution in vivo of the Escherichia coli RNA degradosome
Biochimie, 2013We report an analysis in vivo of the RNA degradosome assembly of Escherichia coli. Employing fluorescence microscopy imaging and fluorescence energy transfer (FRET) measurements, we present evidence for in vivo pairwise interactions between RNase E-PNPase (polynucleotide phosphorylase), and RNase E-Enolase.
Lilianha Domínguez-Malfav´ón +2 more
exaly +3 more sources
Polyphosphate kinase is a component of the Escherichia coli RNA degradosome
Molecular Microbiology, 1997The Escherichia coli degradosome is a multienzyme complex with four major protein components: the endoribonuclease RNase E, the exoribonuclease PNPase, the RNA helicase RhlB and enolase. The first three of these proteins are known to have important functions in mRNA processing and degradation.
E, Blum +3 more
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Analysis of the RNA degradosome complex in Vibrio angustum S14
The FEBS Journal, 2010The RNA degradosome is built on the C‐terminal half of ribonuclease E (RNase E) which shows high sequence variation, even amongst closely related species. This is intriguing given its central role in RNA processing and mRNA decay. Previously, we have identified RhlB (ATP‐dependent DEAD‐box RNA helicase)‐binding, PNPase (polynucleotide phosphorylase ...
Melissa A, Erce +2 more
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Analysis of the Escherichia coli RNA degradosome composition by a proteomic approach
Biochimie, 2006The RNA degradosome is a bacterial protein machine devoted to RNA degradation and processing. In Escherichia coli it is typically composed of the endoribonuclease RNase E, which also serves as a scaffold for the other components, the exoribonuclease PNPase, the RNA helicase RhlB, and enolase.
M.E. Regonesi +7 more
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Preparation of Escherichia coli Rne Protein and Reconstitution of RNA Degradosome
2001Publisher Summary This chapter describes methods for the purification of Rne, the key scaffold in the assembly process, and for the reconstitution of active degradosomes from individually purified enzymes. The endoribonuclease RNase E was initially described as an activity required for the penultimate step in the maturation of 5S rRNA, the processing
G A, Mackie +4 more
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