RNase E biomolecular condensates stimulate PNPase activity [PDF]
Scientific Reports, 2023 Bacterial Ribonucleoprotein bodies (BR-bodies) play an essential role in organizing RNA degradation via phase separation in the cytoplasm of bacteria. BR-bodies mediate multi-step mRNA decay through the concerted activity of the endoribonuclease RNase E ...
Dylan Tomares +2 more
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Polynucleotide phosphorylase has an impact on cell biology of Campylobacter jejuni [PDF]
Frontiers in Cellular and Infection Microbiology, 2012 Polynucleotide phosphorylase, encoded by the pnp gene, is known to degrade mRNA, mediating post-transcriptional regulation and may affect cellular functions. The role of PNPase is pleiotropic.
Nabila eHaddad +12 more
doaj +5 more sources
Microbiology Spectrum, 2022 8-Oxo-7,8-dihydroguanine (8-oxoG) is a major RNA modification caused by oxidative stresses and has been implicated in carcinogenesis, neurodegeneration, and aging.
Runhua Han +3 more
doaj +3 more sources
Selective 8-oxo-rG stalling occurs in the catalytic core of polynucleotide phosphorylase (PNPase) during degradation [PDF]
Proceedings of the National Academy of Sciences of the United States of AmericaRNA oxidation, predominantly through the accumulation of 8-oxo-7,8-dihydroguanosine (8-oxo-rG), represents an important biomarker for cellular oxidative stress. Polynucleotide phosphorylase (PNPase) is a 3′-5′ exoribonuclease that has been shown to preferentially recognize 8-oxo-rG-containing RNA and protectEscherichiacolicells from oxidative stress ...
Runhua Han, Mark Sherman, Haydee Ramirez
exaly +4 more sources
Purine nucleoside phosphorylase as a target for the treatment of interstitial cystitis/bladder pain syndrome with and without Hunner lesions [PDF]
Scientific ReportsChronic visceral pain disorders, such as interstitial cystitis/bladder pain syndrome (IC/BPS), are difficult to treat, and therapies are limited in number and efficacy.
Lori A. Birder +6 more
doaj +2 more sources
Trafficking of mitochondrial double-stranded RNA from mitochondria to the cytosol [PDF]
Life Science AllianceMitochondrial double-stranded RNA is a new signaling molecule that, likely under stress conditions, is exported to induce a type 1 interferon response, thus mimicking a viral response. This export pathway is detected in a subset of lung cancer cell lines.
Matthew R Krieger +13 more
doaj +2 more sources
Rapid and scalable detection of synthetic mRNA byproducts using polynucleotide phosphorylase and polythymidine oligonucleotides [PDF]
RNA BiologyProduction and storage of synthetic mRNA can introduce a variety of byproducts which reduce the overall integrity and functionality of mRNA vaccines and therapeutics.
Francis Combes +3 more
doaj +2 more sources
BMC Genomics, 2021 Background The polynucleotide phosphorylase (PNPase) is conserved among both Gram-positive and Gram-negative bacteria. As a core part of the Escherichia coli degradosome, PNPase is involved in maintaining proper RNA levels within the bacterial cell.
Daniel-Timon Spanka +2 more
doaj +1 more source
Polynucleotide phosphorylase: Not merely an RNase but a pivotal post-transcriptional regulator. [PDF]
PLoS Genetics, 2018 Almost 60 years ago, Severo Ochoa was awarded the Nobel Prize in Physiology or Medicine for his discovery of the enzymatic synthesis of RNA by polynucleotide phosphorylase (PNPase).
Todd A Cameron +2 more
doaj +1 more source
Isolate Specific Cold Response of Yersinia enterocolitica in Transcriptional, Proteomic, and Membrane Physiological Changes [PDF]
, 2020 Yersinia enterocolitica, a zoonotic foodborne pathogen, is able to withstand low temperatures. This psychrotrophic ability allows it to multiply in food stored in refrigerators. However, little is known about the Y. enterocolitica cold response.
Alter, Thomas +4 more
core +1 more source