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RNase E biomolecular condensates stimulate PNPase activity
Scientific Reports, 2023 Bacterial Ribonucleoprotein bodies (BR-bodies) play an essential role in organizing RNA degradation via phase separation in the cytoplasm of bacteria. BR-bodies mediate multi-step mRNA decay through the concerted activity of the endoribonuclease RNase E ...
Michael J. Collins +4 more
doaj +3 more sources
Human PNPase causes RNA stabilization and accumulation of R-loops in the Escherichia coli model system [PDF]
Scientific Reports, 2023 Polyribonucleotide phosphorylase (PNPase) is a phosphorolytic RNA exonuclease highly conserved throughout evolution. In Escherichia coli, PNPase controls complex phenotypic traits like biofilm formation and growth at low temperature.
Federica A. Falchi +7 more
doaj +3 more sources
The ribonuclease PNPase is a key regulator of biofilm formation in Listeria monocytogenes and affects invasion of host cells [PDF]
npj Biofilms and Microbiomes, 2023 Biofilms provide an environment that protects microorganisms from external stresses such as nutrient deprivation, antibiotic treatments, and immune defences, thereby creating favorable conditions for bacterial survival and pathogenesis. Here we show that
Ana Patrícia Quendera +6 more
doaj +3 more sources
Microbiology Spectrum, 2022 8-Oxo-7,8-dihydroguanine (8-oxoG) is a major RNA modification caused by oxidative stresses and has been implicated in carcinogenesis, neurodegeneration, and aging.
Lydia Contreras, Runhua Han
exaly +4 more sources
Selective 8-oxo-rG stalling occurs in the catalytic core of polynucleotide phosphorylase (PNPase) during degradation [PDF]
Proceedings of the National Academy of Sciences of the United States of AmericaSignificance Polynucleotide phosphorylase (PNPase) is a widely conserved exoribonuclease that degrades single-stranded RNA transcripts in the 3′ to 5′ direction, forming single-nucleotide diphosphates and a short oligonucleotide product.
Lydia Contreras +2 more
exaly +3 more sources
Molecular and Cellular Oncology, 2018 Transcription of the human mitochondrial genome produces a vast amount of non-coding antisense RNAs. These RNA species can form G-quadraplexes (G4), which affect their decay. We found that the mitochondrial degradosome, a complex of RNA helicase SUPV3L1 (
Magdalena Wójcik +2 more
exaly +3 more sources
BMC Genomics, 2021 Background The polynucleotide phosphorylase (PNPase) is conserved among both Gram-positive and Gram-negative bacteria. As a core part of the Escherichia coli degradosome, PNPase is involved in maintaining proper RNA levels within the bacterial cell.
Daniel-Timon Spanka +2 more
doaj +2 more sources
Loop-mediated regulation and base flipping drive RNA cleavage by human mitochondrial PNPase. [PDF]
Nucleic Acids ResHuman polynucleotide phosphorylase (hPNPase), a trimeric exoribonuclease, is crucial for maintaining mitochondrial RNA metabolism, including the regulated degradation of RNA.
Unseld O, Das H, Hällberg BM.
europepmc +2 more sources
A glycolytic intermediate gatekeeps global mRNA decay in Escherichia coli [PDF]
Nature CommunicationsPolynucleotide phosphorylase (PNPase) is a highly conserved exoribonuclease crucial for RNA degradation across all domains of life. In bacteria, PNPase is found in multi-protein ribonucleolytic complexes that include glycolytic enzymes.
Ander Hernández Plágaro +9 more
doaj +2 more sources
Nature Communications, 2023 Observing the natural process of RNA degradation in real-time is a significant challenge. Here, the authors develop and use single-exonuclease nanocircuits to reveal the single-base degradation behaviour of PNPase, and demonstrate proof-of-principle RNA ...
Zhiheng Yang +6 more
doaj +2 more sources