Results 21 to 30 of about 4,319 (202)

Human FASTK preferentially binds single-stranded and G-rich RNA. [PDF]

FEBS J
Although FASTK is known to play a role in mRNA biology, the mechanism through which it recognizes RNA has yet to be unraveled. Here, we used purified human FASTK to characterize its RNA‐binding properties in vitro. We found that FASTK prefers ssRNA oligonucleotides containing guanines with the potential to form G‐quadruplexes and binds robustly to any ...
Dawidziak DM, Dzadz DA, Kuska MI, Kanavalli M, Klimecka MM, Merski M, Bandyra KJ, Górna MW.   +7 more
europepmc   +2 more sources

The ribonuclease PNPase is a key regulator of biofilm formation in Listeria monocytogenes and affects invasion of host cells

npj Biofilms and Microbiomes, 2023
Biofilms provide an environment that protects microorganisms from external stresses such as nutrient deprivation, antibiotic treatments, and immune defences, thereby creating favorable conditions for bacterial survival and pathogenesis. Here we show that
Ana Patrícia Quendera, Sandra Nunes Pinto, Vânia Pobre, Wilson Antunes, Vasco D. B. Bonifácio, Cecília Maria Arraiano, José Marques Andrade   +6 more
doaj   +1 more source

PNPase degradation dynamic analysis

, 2023
The electrical detecion data and analyized data have been included in this file.
Zhihengyang, Xuefeng Guo
openaire   +1 more source

Analysis of mRNA Decay Intermediates in Bacillus subtilis 3′ Exoribonuclease and RNA Helicase Mutant Strains

mBio, 2022
The Bacillus subtilis genome encodes four 3′ exoribonucleases: polynucleotide phosphorylase (PNPase), RNase R, RNase PH, and YhaM. Previous work showed that PNPase, encoded by the pnpA gene, is the major 3′ exonuclease involved in mRNA turnover; in a ...
Shivani Chhabra, Zachary F. Mandell, Bo Liu, Paul Babitzke, David H. Bechhofer   +4 more
doaj   +1 more source

Polynucleotide Phosphorylase Mediates a New Mechanism of Persister Formation in Escherichia coli

Microbiology Spectrum, 2023
Despite the identification of many genes and pathways involved in the persistence phenomenon in bacteria, the mechanisms of persistence are not well understood.
Nan Wu, Yumeng Zhang, Shanshan Zhang, Youhua Yuan, Shuang Liu, Tao Xu, Peng Cui, Wenhong Zhang, Ying Zhang   +8 more
doaj   +1 more source

Heterozygous PNPT1 Variants Cause a Sensory Ataxic Neuropathy. [PDF]

Eur J Neurol
ABSTRACT Background Biallelic variants in polyribonucleotide‐nucleotidyltransferase‐1 (PNPT1) have been associated with a range of phenotypes from syndromic hearing loss to Leigh's syndrome. More recently, heterozygous variants in PNPT1, have been reported in three families with cerebellar ataxia and prominent sensory neuropathy.
Haddad S, Record CJ, Self E, Skorupinska M, Rossor AM, Laura M, Ingle G, Manzur A, Muntoni F, Blake JC, Reilly MM.   +10 more
europepmc   +2 more sources

Novel Aspects of Polynucleotide Phosphorylase Function in Streptomyces

Antibiotics, 2018
Polynucleotide phosphorylase (PNPase) is a 3′–5′-exoribnuclease that is found in most bacteria and in some eukaryotic organelles. The enzyme plays a key role in RNA decay in these systems.
George H. Jones
doaj   +1 more source

Different csrA Expression Levels in C versus K-12 E. coli Strains Affect Biofilm Formation and Impact the Regulatory Mechanism Presided by the CsrB and CsrC Small RNAs

Microorganisms, 2021
Escherichia coli C is a strong biofilm producer in comparison to E. coli K-12 laboratory strains due to higher expression of the pgaABCD operon encoding the enzymes for the biosynthesis of the extracellular polysaccharide poly-β-1,6-N-acetylglucosamine ...
Thomas Carzaniga, Federica A. Falchi, Francesca Forti, Davide Antoniani, Paolo Landini, Federica Briani   +5 more
doaj   +1 more source

Human mitochondrial degradosome prevents harmful mitochondrial R loops and mitochondrial genome instability [PDF]

, 2018
R loops are nucleic acid structures comprising an DNA-RNA hybrid and a displaced single-stranded DNA. These structures may occur transiently during transcription, playing essential biological functions. However, persistent R loops may become pathological
Aguilera López, Andrés, Pérez de Camino Cantos, Dolores, Silva, Sonia   +2 more
core   +1 more source

PNPase is involved in the coordination of mRNA degradation and expression in stationary phase cells of Escherichia coli

BMC Genomics, 2018
Background Exoribonucleases are crucial for RNA degradation in Escherichia coli but the roles of RNase R and PNPase and their potential overlap in stationary phase are not well characterized.
Clémentine Dressaire, Vânia Pobre, Sandrine Laguerre, Laurence Girbal, Cecilia Maria Arraiano, Muriel Cocaign-Bousquet   +5 more
doaj   +1 more source