Results 1 to 10 of about 4,051 (196)

Human mitochondrial degradosome prevents harmful mitochondrial R loops and mitochondrial genome instability [PDF]

Proceedings of the National Academy of Sciences of the United States of America, 2018
R loops are nucleic acid structures comprising an DNA-RNA hybrid and a displaced single-stranded DNA. These structures may occur transiently during transcription, playing essential biological functions. However, persistent R loops may become pathological
Sonia Silva, Lola P Camino, Andres Aguilera   +2 more
exaly   +10 more sources

Localization of components of the RNA-degrading machine in Bacillus subtilis [PDF]

Frontiers in Microbiology, 2016
In bacteria, the control of mRNA stability is crucial to allow rapid adaptation to changing conditions. In most bacteria, RNA degradation is catalyzed by the RNA degradosome, a protein complex composed of endo- and exoribonucleases, RNA helicases and ...
Nora Cascante-Estepa, Katrin Gunka, Jörg Stülke   +2 more
doaj   +7 more sources

The DEAD-box RNA helicase RhlB is required for efficient RNA processing at low temperature in Caulobacter [PDF]

Microbiology Spectrum, 2023
The RNA helicases from the DEAD-box family participate in several biochemical processes, with special relevance in the assembly of ribosome ribonucleoparticles and in RNA turnover as part of the multi-enzymatic RNA degradosome complex.
Hugo L. de Araújo, Beatriz A. Picinato, Alan P. R. Lorenzetti, Nisansala S. Muthunayake, I. W. Rathnayaka-Mudiyanselage, Naara M. dos Santos, Jared Schrader, Tie Koide, Marilis V. Marques   +8 more
doaj   +2 more sources

Structure of the 30S translation initiation complex coupled to paused RNA polymerase and its potential for riboregulation [PDF]

Nature Communications
In many bacterial species, transcription and translation can be coupled physically, with potential impact on the rates and efficiency of gene expression. Here, we present structural evidence from cryo-EM demonstrating that a bacterial RNA polymerase that
Johann J. Roske, Giulia Paris, Akanksha Goyal, Marina Rodnina, Nikolay Zenkin, Katarzyna J. Bandyra, Ben F. Luisi   +6 more
doaj   +3 more sources

Critical functions and key interactions mediated by the RNase E scaffolding domain in Pseudomonas aeruginosa. [PDF]

PLoS Genetics
The RNA degradosome is a bacterial multi-protein complex mediating mRNA processing and degradation. In Pseudomonadota, this complex assembles on the C-terminal domain (CTD) of RNase E through short linear motifs (SLiMs) that determine its composition and
Sandra Amandine Marie Geslain, Stéphane Hausmann, Johan Geiser, George Edward Allen, Diego Gonzalez, Martina Valentini   +5 more
doaj   +2 more sources

The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies [PDF]

Microbial Cell Factories, 2005
Background The permanently impaired protein folding during recombinant protein production resembles the stress encountered at extreme temperatures, under which condition the putative holding chaperones, IbpA/IbpB, play an important role. We evaluated the
Hoffmann Frank, Neubauer Peter, LeThanh Ha   +2 more
doaj   +2 more sources

The BR-body proteome contains a complex network of protein-protein and protein-RNA interactions [PDF]

Cell Reports, 2023
Summary: Bacterial ribonucleoprotein bodies (BR-bodies) are non-membrane-bound structures that facilitate mRNA decay by concentrating mRNA substrates with RNase E and the associated RNA degradosome machinery.
Vidhyadhar Nandana, Imalka W. Rathnayaka-Mudiyanselage, Nisansala S. Muthunayake, Ali Hatami, C. Bruce Mousseau, Luis A. Ortiz-Rodríguez, Jamuna Vaishnav, Michael Collins, Alisa Gega, Kaveendya S. Mallikaarachchi, Hadi Yassine, Aishwarya Ghosh, Julie S. Biteen, Yingxi Zhu, Matthew M. Champion, W. Seth Childers, Jared M. Schrader   +16 more
doaj   +2 more sources

Formation of a membraneless compartment regulates bacterial virulence [PDF]

Nature Communications
The RNA-binding protein CsrA regulates the expression of hundreds of genes in several bacterial species, thus controlling virulence and other processes.
Lior Aroeti, Netanel Elbaz, Raya Faigenbaum-Romm, Oren Yakovian, Yael Altuvia, Liron Argaman, Naama Katsowich, Michal Bejerano-Sagie, Miriam Ravins, Hanah Margalit, Sigal Ben-Yehuda, Ilan Rosenshine   +11 more
doaj   +2 more sources

Mechanistic insights into recruitment and regulation of the RNA helicase UPF1 in replication-dependent histone mRNA decay [PDF]

Nature Communications
Metazoan histone mRNAs are a unique class of mRNAs that lack the poly(A) tail present in all other eukaryotic transcripts. Instead, they end in a conserved stem-loop (SL) structure, necessitating a decay mechanism that is distinct from deadenylation ...
Alexandrina Machado de Amorim, Guangpu Xue, Wenxia He, Theresa Dittmers, Sarah Lewandowski, Cecilia Perez-Borrajero, Juliane Bethmann, Nevena Mateva, Clemens Krage, Vidhyadhar Nandana, Bernhard Loll, Tarek Hilal, Janosch Hennig, Henning Urlaub, William F. Marzluff, Sutapa Chakrabarti   +15 more
doaj   +2 more sources

Polyribosome-Dependent Clustering of Membrane-Anchored RNA Degradosomes To Form Sites of mRNA Degradation in Escherichia coli

mBio, 2021
Here, we show that RNase E, RhlB, and PNPase act together as components of the multienzyme RNA degradosome in polyribosome-dependent clustering to form puncta on the inner cytoplasmic membrane.
Lina Hamouche, Leonora Poljak, Agamemnon J. Carpousis   +2 more
doaj   +1 more source