Results 11 to 20 of about 4,051 (196)

Membrane recognition and dynamics of the RNA degradosome.

PLoS Genetics, 2015
RNase E, which is the central component of the multienzyme RNA degradosome, serves as a scaffold for interaction with other enzymes involved in mRNA degradation including the DEAD-box RNA helicase RhlB.
Henrik Strahl, Catherine Turlan, Syma Khalid, Peter J Bond, Jean-Marie Kebalo, Pascale Peyron, Leonora Poljak, Marie Bouvier, Leendert Hamoen, Ben F Luisi, Agamemnon J Carpousis   +10 more
doaj   +6 more sources

A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunity

eLife, 2019
CRISPR-Cas systems provide sequence-specific immunity against phages and mobile genetic elements using CRISPR-associated nucleases guided by short CRISPR RNAs (crRNAs).
Lucy Chou-Zheng, Asma Hatoum-Aslan
doaj   +3 more sources

Human FASTK preferentially binds single-stranded and G-rich RNA. [PDF]

FEBS J
Although FASTK is known to play a role in mRNA biology, the mechanism through which it recognizes RNA has yet to be unraveled. Here, we used purified human FASTK to characterize its RNA‐binding properties in vitro. We found that FASTK prefers ssRNA oligonucleotides containing guanines with the potential to form G‐quadruplexes and binds robustly to any ...
Dawidziak DM, Dzadz DA, Kuska MI, Kanavalli M, Klimecka MM, Merski M, Bandyra KJ, Górna MW.   +7 more
europepmc   +2 more sources

Multi‐scale ensemble properties of the Escherichia coli RNA degradosome [PDF]

Molecular Microbiology, 2021
AbstractIn organisms from all domains of life, multi‐enzyme assemblies play central roles in defining transcript lifetimes and facilitating RNA‐mediated regulation of gene expression. An assembly dedicated to such roles, known as the RNA degradosome, is found amongst bacteria from highly diverse lineages.
Dendooven, Tom, Paris, Giulia, Shkumatov, Alexander V., Islam, Md. Saiful, Burt, Alister, Kubańska, Marta A., Yang, Tai Yuchen, Hardwick, Steven W., Luisi, Ben F.   +8 more
openaire   +4 more sources

The ribonuclease E regulator RebA is essential for diazotrophic growth in the cyanobacterium <i>Anabaena</i> PCC 7120. [PDF]

mLife
Abstract Ribonuclease E (RNase E) is central to bacterial RNA metabolism. In cyanobacteria, its activity is inhibited by RebA, a key mechanism for controlling cell morphology. Here, we demonstrate that rebA is essential for diazotrophic growth of Anabaena PCC 7120, a filamentous cyanobacterium capable of forming heterocysts—specialized nitrogen‐fixing ...
Liu S, Wang Z, Lin G, Li W, Zeng X, Zhang JY, Zhang CC.   +6 more
europepmc   +2 more sources

Bacterial RNA Degradosomes: Molecular Machines under Tight Control [PDF]

Trends in Biochemical Sciences, 2020
Bacterial RNA degradosomes are multienzyme molecular machines that act as hubs for post-transcriptional regulation of gene expression. The ribonuclease activities of these complexes require tight regulation, as they are usually essential for cell survival while potentially destructive.
Tejada Arranz, Alejandro, de Crecy-Lagard, Valerie, de Reuse, Hilde   +2 more
openaire   +2 more sources

Moonlighting in Bacillus subtilis: The Small Proteins SR1P and SR7P Regulate the Moonlighting Activity of Glyceraldehyde 3-Phosphate Dehydrogenase A (GapA) and Enolase in RNA Degradation

Microorganisms, 2021
Moonlighting proteins are proteins with more than one function. During the past 25 years, they have been found to be rather widespread in bacteria. In Bacillus subtilis, moonlighting has been disclosed to occur via DNA, protein or RNA binding or protein ...
Inam Ul Haq, Sabine Brantl
doaj   +1 more source

An RNA degradosome assembly in Caulobacter crescentus [PDF]

Nucleic Acids Research, 2010
In many bacterial species, the multi-enzyme RNA degradosome assembly makes key contributions to RNA metabolism. Powering the turnover of RNA and the processing of structural precursors, the RNA degradosome has differential activities on a spectrum of transcripts and contributes to gene regulation at a global level.
Hardwick, Steven W., Chan, Vivian S. Y., Broadhurst, R. William, Luisi, Ben F.   +3 more
openaire   +2 more sources

Attachment of the RNA degradosome to the bacterial inner cytoplasmic membrane prevents wasteful degradation of rRNA in ribosome assembly intermediates.

PLoS Biology, 2023
RNA processing and degradation shape the transcriptome by generating stable molecules that are necessary for translation (rRNA and tRNA) and by facilitating the turnover of mRNA, which is necessary for the posttranscriptional control of gene expression ...
Lydia Hadjeras, Marie Bouvier, Isabelle Canal, Leonora Poljak, Quentin Morin-Ogier, Carine Froment, Odile Burlet-Schlitz, Lina Hamouche, Laurence Girbal, Muriel Cocaign-Bousquet, Agamemnon J Carpousis   +10 more
doaj   +1 more source

Cooperation of regulatory RNA and the RNA degradosome in transcript surveillance. [PDF]

Nucleic Acids Res
Abstract The ompD transcript, encoding an outer membrane porin in Salmonella, harbors a controlling element in its coding region that base-pairs imperfectly with a ‘seed’ region of the small regulatory RNA (sRNA) MicC. When tagged with the sRNA, the ompD mRNA is cleaved downstream of the pairing site by the conserved endoribonuclease ...
Bandyra KJ, Fröhlich KS, Vogel J, Rodnina M, Goyal A, Luisi BF.   +5 more
europepmc   +9 more sources