Results 11 to 20 of about 3,184 (153)

Membrane recognition and dynamics of the RNA degradosome. [PDF]

PLoS Genetics, 2015
RNase E, which is the central component of the multienzyme RNA degradosome, serves as a scaffold for interaction with other enzymes involved in mRNA degradation including the DEAD-box RNA helicase RhlB.
Henrik Strahl, Catherine Turlan, Syma Khalid, Peter J Bond, Jean-Marie Kebalo, Pascale Peyron, Leonora Poljak, Marie Bouvier, Leendert Hamoen, Ben F Luisi, Agamemnon J Carpousis   +10 more
doaj   +3 more sources

Human FASTK preferentially binds single-stranded and G-rich RNA. [PDF]

FEBS J
Although FASTK is known to play a role in mRNA biology, the mechanism through which it recognizes RNA has yet to be unraveled. Here, we used purified human FASTK to characterize its RNA‐binding properties in vitro. We found that FASTK prefers ssRNA oligonucleotides containing guanines with the potential to form G‐quadruplexes and binds robustly to any ...
Dawidziak DM, Dzadz DA, Kuska MI, Kanavalli M, Klimecka MM, Merski M, Bandyra KJ, Górna MW.   +7 more
europepmc   +2 more sources

Biophysical characterization of the Escherichia coli RNA degradosome [PDF]

, 2022
In Escherichia coli, post-transcriptional regulation is a tightly controlled process facilitated by a multi-enzyme complex, the RNA degradosome. The core components of the RNA degradosome consist of the endoribonuclease RNase E, the DEAD-box helicase ...

core   +2 more sources

Multi-scale ensemble properties of the Escherichia coli RNA degradosome. [PDF]

, 2021
In organisms from all domains of life, multi-enzyme assemblies play central roles in defining transcript lifetimes and facilitating RNA-mediated regulation of gene expression.
Yang, Tai Yuchen, Paris, Giulia, Shkumatov, Alexander V., Kubańska, Marta A., Hardwick, Steven W., Luisi, Ben F., Islam, Md. Saiful, Dendooven, Tom, Burt, Alister   +8 more
core   +4 more sources

The ribonuclease E regulator RebA is essential for diazotrophic growth in the cyanobacterium <i>Anabaena</i> PCC 7120. [PDF]

mLife
Abstract Ribonuclease E (RNase E) is central to bacterial RNA metabolism. In cyanobacteria, its activity is inhibited by RebA, a key mechanism for controlling cell morphology. Here, we demonstrate that rebA is essential for diazotrophic growth of Anabaena PCC 7120, a filamentous cyanobacterium capable of forming heterocysts—specialized nitrogen‐fixing ...
Liu S, Wang Z, Lin G, Li W, Zeng X, Zhang JY, Zhang CC.   +6 more
europepmc   +2 more sources

Cooperation of regulatory RNA and the RNA degradosome in transcript surveillance. [PDF]

Nucleic Acids Res
The ompD transcript, encoding an outer membrane porin in Salmonella, harbors a controlling element in its coding region that base-pairs imperfectly with a 'seed' region of the small regulatory RNA (sRNA) MicC.
Bandyra KJ, Fröhlich KS, Vogel J, Rodnina M, Goyal A, Luisi BF.   +5 more
europepmc   +20 more sources

A multi-dentate, cooperative interaction between endo- and exo-ribonucleases within the bacterial RNA degradosome. [PDF]

Nucleic Acids Res
In Escherichia coli and numerous other bacteria, two of the principal enzymes mediating messenger RNA decay and RNA processing-RNase E, an endoribonuclease, and polynucleotide phosphorylase (PNPase), an exoribonuclease-assemble into a multi-enzyme ...
Paris G, Katsuya-Gaviria K, Clarke H, Johncock M, Dendooven T, Lulla A, Luisi BF.   +6 more
europepmc   +3 more sources

The RNase J-Based RNA Degradosome Is Compartmentalized in the Gastric Pathogen Helicobacter pylori

mBio, 2020
Posttranscriptional regulation is a major level of gene expression control in any cell. In bacteria, multiprotein machines called RNA degradosomes are central for RNA processing and degradation, and some were reported to be compartmentalized inside these
Alejandro Tejada-Arranz, Eloïse Galtier, Lamya El Mortaji, Evelyne Turlin, Dmitry Ershov, Hilde De Reuse   +5 more
doaj   +1 more source

Analysis of mRNA Decay Intermediates in Bacillus subtilis 3′ Exoribonuclease and RNA Helicase Mutant Strains

mBio, 2022
The Bacillus subtilis genome encodes four 3′ exoribonucleases: polynucleotide phosphorylase (PNPase), RNase R, RNase PH, and YhaM. Previous work showed that PNPase, encoded by the pnpA gene, is the major 3′ exonuclease involved in mRNA turnover; in a ...
Shivani Chhabra, Zachary F. Mandell, Bo Liu, Paul Babitzke, David H. Bechhofer   +4 more
doaj   +1 more source

The Escherichia coli major exoribonuclease RNase II is a component of the RNA degradosome

Bioscience Reports, 2014
Multiprotein complexes that carry out RNA degradation and processing functions are found in cells from all domains of life. In Escherichia coli, the RNA degradosome, a four-protein complex, is required for normal RNA degradation and processing.
Feng Lu, Aziz Taghbalout
doaj   +1 more source