Results 21 to 30 of about 4,051 (196)

The RNase J-Based RNA Degradosome Is Compartmentalized in the Gastric Pathogen Helicobacter pylori

mBio, 2020
Posttranscriptional regulation is a major level of gene expression control in any cell. In bacteria, multiprotein machines called RNA degradosomes are central for RNA processing and degradation, and some were reported to be compartmentalized inside these
Alejandro Tejada-Arranz, Eloïse Galtier, Lamya El Mortaji, Evelyne Turlin, Dmitry Ershov, Hilde De Reuse   +5 more
doaj   +1 more source

Analysis of mRNA Decay Intermediates in Bacillus subtilis 3′ Exoribonuclease and RNA Helicase Mutant Strains

mBio, 2022
The Bacillus subtilis genome encodes four 3′ exoribonucleases: polynucleotide phosphorylase (PNPase), RNase R, RNase PH, and YhaM. Previous work showed that PNPase, encoded by the pnpA gene, is the major 3′ exonuclease involved in mRNA turnover; in a ...
Shivani Chhabra, Zachary F. Mandell, Bo Liu, Paul Babitzke, David H. Bechhofer   +4 more
doaj   +1 more source

α-Proteobacterial RNA Degradosomes Assemble Liquid-Liquid Phase-Separated RNP Bodies [PDF]

Molecular Cell, 2018
Ribonucleoprotein (RNP) granules play an important role in organizing eukaryotic mRNA metabolism via liquid-liquid phase separation (LLPS) of mRNA decay factors into membrane-less organelles in the cytoplasm. Here we show that the bacterium Caulobacter crescentus Ribonuclease (RNase) E assembles RNP LLPS condensates that we term bacterial RNP-bodies ...
Nadra, Al-Husini, Dylan T, Tomares, Obaidah, Bitar, W Seth, Childers, Jared M, Schrader   +4 more
openaire   +3 more sources

TheYersinia pseudotuberculosisDegradosome is Required for Oxidative Stress, While its PNPase Subunit Plays a Degradosome-Independent Role in Cold Growth [PDF]

FEMS Microbiology Letters, 2012
Yersinia polynucleotide phosphorylase (PNPase), a 3'-5' exoribonuclease, has been shown to affect growth during several stress responses. In Escherichia coli, PNPase is one of the subunits of a multiprotein complex known as the degradosome, but also has degradosome-independent functions. The carboxy-terminus of E.
Amanda, Henry, Justin, Shanks, Ambro, van Hoof, Jason A, Rosenzweig   +3 more
openaire   +2 more sources

The exoribonuclease Polynucleotide Phosphorylase influences the virulence and stress responses of yersiniae and many other pathogens

Frontiers in Cellular and Infection Microbiology, 2013
Microbes are incessantly challenged by both biotic and abiotic stressors threatening their existence. Therefore, bacterial pathogens must possess mechanisms to successfully subvert host immune defenses as well as overcome the stress associated with host ...
Jason A. Rosenzweig, Ashok K Chopra
doaj   +1 more source

Conformational Isomerization Involving Conserved Proline Residues Modulates Oligomerization of the NS1 Interferon Response Inhibitor from the Syncytial Respiratory Virus [PDF]

, 2019
Interferon response suppression by the respiratory syncytial virus relies on two unique nonstructural proteins, NS1 and NS2, that interact with cellular partners through high-order complexes.
Alonso, Leonardo Gabriel, Aran, Martin, Borkosky, Silvina Susana, Conci, Julieta, de Oliveira, Guilherme A. P., de Prat Gay, Gonzalo, Esperante, Sebastian, Mohana Borges, Ronaldo, Pagani, Talita Duarte, Álvarez Paggi, Damián Jorge   +9 more
core   +1 more source

The proteomic response to mutants of the Escherichia coli RNA degradosome [PDF]

Molecular BioSystems, 2013
The Escherichia coli RNA degradosome recognizes and degrades RNA through the coordination of four main protein components, the endonuclease RNase E, the exonuclease PNPase, the RhlB helicase and the metabolic enzyme enolase. To help our understanding of the functions of the RNA degradosome, we quantified expression changes of >2300 proteins using mass ...
Li, Zhou, Ang B, Zhang, Rong, Wang, Edward M, Marcotte, Christine, Vogel   +4 more
openaire   +2 more sources

Distinct co-evolution patterns of genes associated to DNA polymerase III DnaE and PolC

BMC Genomics, 2012
Background Bacterial genomes displaying a strong bias between the leading and the lagging strand of DNA replication encode two DNA polymerases III, DnaE and PolC, rather than a single one.
Engelen Stefan, Vallenet David, Médigue Claudine, Danchin Antoine   +3 more
doaj   +1 more source

A new yeast poly(A) polymerase complex involved in RNA quality control [PDF]

, 2005
Eukaryotic cells contain several unconventional poly(A) polymerases in addition to the canonical enzymes responsible for the synthesis of poly(A) tails of nuclear messenger RNA precursors.
Štěpánka Vaňáčová, Jeannette Wolf, Georges Martin, Diana Blank, Sabine Dettwiler, Arno Friedlein, Hanno Langen, Gérard Keith, Walter Keller, Phillip Zamore, Wahle, Minvielle-Sebastia, Zhao, Edmonds, Proudfoot, Zorio, Aravind, Holm, Martin, Preker, Martin, Martin, Bard, Read, Saitoh, Wang, Sadoff, Wang, Wang, Crittenden, Kwak, Keller, van Hoof, Kuai, Kadaba, Mitchell, O'Hara, Li, Cheng, Dreyfus, Inoue, Martin, Puig, Lingner, de la Cruz, Wang, Vijayraghavan, Anderson, Gerber, Dirheimer, Mitchell, Walowsky, Dannull, Amarasinghe, Castaño, Basavappa, Kushner, Symmons, Parker, Torchet, Fromont-Racine, Ohnacker, Dichtl, Fountoulakis, Wilm, Dirheimer, Keith, Senger, Dichtl, Christofori, Falquet, Rain   +71 more
core   +4 more sources

LEVELNET to visualize, explore, and compare protein–protein interaction networks

PROTEOMICS, Volume 23, Issue 17, September 2023., 2023
Abstract Physical interactions between proteins are central to all biological processes. Yet, the current knowledge of who interacts with whom in the cell and in what manner relies on partial, noisy, and highly heterogeneous data. Thus, there is a need for methods comprehensively describing and organizing such data.
Yasser Mohseni Behbahani, Paul Saighi, Flavia Corsi, Elodie Laine, Alessandra Carbone   +4 more
wiley   +1 more source