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Enolase isoenzymes in human gliomas
Journal of Neurosurgery, 1986✓ Gamma-enolase (one of the three possible subunits of the dimeric enzyme enolase (EC 4.2.1.11)) has been reported as a marker for human neurons. Studies investigating the presence of γ-enolase in human gliomas have given conflicting results, but a definite finding is important for further studies of the biology of these tumors and the possible use of ...
E M, van den Doel +4 more
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Rat brain enolase isozymes. Purification of three forms of enolase.
Journal of biochemistry, 1980Three forms of rat brain enolase (Fletcher, L., Rider, C.C., & Taylor, C.B. (1976) Biochim. Biophys. Acta 452, 245-252), which were separable by DEAE-cellulose chromatography (referred as enolase I, enolase II, and enolase III in order of the elution), were purified with a high yield by use of column chromatographies of Sephadex G-150, Blue Sepharose ...
F, Suzuki, Y, Umeda, K, Kato
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Reaction intermediate analogs for enolase
Biochemistry, 1984A number of compounds that appear to be analogues of the aci form of the normal carbanion intermediate are good inhibitors of yeast enolase. These include (3-hydroxy-2-nitropropyl)phosphonate (I), the ionized (pK = 8.1) nitronate form of which in the presence of 5 mM Mg2+ has a Ki of 6 nM, (nitroethyl)phosphonate (III) (pK = 8.5; Ki of the nitronate in
V E, Anderson, P M, Weiss, W W, Cleland
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Phosphonate analog substrates for enolase
Biochemistry, 1990Phosphonate analogues in which the bridge between C-2 and phosphorus is a CH2 group are slow substrates for yeast enolase. The pH variation of the kinetic parameters for the methylene analogue of 2-phosphoglycerate suggests that the substrate binds as a dianion and that Mg2+ can bind subsequently only if a metal ligand and the catalytic base are ...
V E, Anderson, W W, Cleland
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Phosphorylation of Escherichia coli enolase
Biochimie, 1989In vivo labeling of Escherichia coli JA200 pLC 11-8 resulted in 32P incorporation into enolase as demonstrated by immunoaffinity chromatography and electrophoresis followed by autoradiography. Complete acid hydrolysis, followed by thin layer chromatography was employed for determination of the phosphoamino acid residue.
H K, Dannelly +3 more
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Biochimie, 2011
To detect proteins binding to CUG triplet repeats, we performed magnetic bead affinity assays and North-Western analysis using a (CUG)(10) ssRNA probe and either nuclear or total extracts from rat L6 myoblasts. We report the isolation and identification by mass spectrometry and immunodetection of α-enolase, as a novel (CUG)n triplet repeat binding ...
Liliana, Hernández-Pérez +6 more
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To detect proteins binding to CUG triplet repeats, we performed magnetic bead affinity assays and North-Western analysis using a (CUG)(10) ssRNA probe and either nuclear or total extracts from rat L6 myoblasts. We report the isolation and identification by mass spectrometry and immunodetection of α-enolase, as a novel (CUG)n triplet repeat binding ...
Liliana, Hernández-Pérez +6 more
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Enolase-1 as a plasminogen receptor
Blood, 2009In this issue of Blood, Wygrecka and colleagues assign a major role to cell-surface enolase-1 as a plasminogen receptor, mediating LPS-induced invasion of monocytes into lungs in mice and humans.
Edward F, Plow, Riku, Das
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Enolase isozymes in Coho salmon
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 19781. Analysis of the enolase isozymic distribution has been performed in tissues of the Coho salmon, using electrophoretic separation on cellulose acetate strips followed by localization of enzymatic activity. 2. A total of six electrophoretically distinct forms are seen in Coho salmon in patterns that differ both qualitatively and quantitatively from ...
S R, Landrey +2 more
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[Neuron-specific enolase in ophthalmology].
Archivio italiano di anatomia e di embriologia. Italian journal of anatomy and embryology, 1991Tissue location and biochemical aspects of the enolase, enzyme belonging to the glycolytic pathway, are presented. Embryological applications of the dimer gamma-gamma (Neuronal Specific Enolase) as a biological marker of the neuroectodermal derivatives is discussed. The authors stress the clinical importance of the NSE in the early detection of tumors.
P. Nucci +4 more
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Interaction of Manganous Ions with Enolase
Nature, 1953MANY enzymes require the presence of metal ions for activity1. Kinetic studies on such systems indicate the existence of an equilibrium between the enzyme protein, the activating metal ion and a complex between metal ion and enzyme. By assuming the complex to be the only species catalytically active, a hypothetical dissociation constant can be ...
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