Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly [PDF]
Open Biology, 2012 Polynucleotide phosphorylase (PNPase) is an exoribonuclease that cleaves single-stranded RNA substrates with 3′–5′ directionality and processive behaviour.
Steven W. Hardwick +4 more
doaj +1 more source
The first small-molecule inhibitors of members of the ribonuclease E family [PDF]
, 2015 The Escherichia coli endoribonuclease RNase E is central to the processing and degradation of all types of RNA and as such is a pleotropic regulator of gene expression.
A Danchin +43 more
core +4 more sources
Inflammatory mitochondrial nucleic acids as drivers of pathophysiology
, 2023 Clinical and Translational Medicine, Volume 13, Issue 9, September 2023.
Christian G. Peace +2 more
wiley +1 more source
Critical roles for ‘housekeeping’ nucleases in type III CRISPR-Cas immunity
eLife, 2022 CRISPR-Cas systems are a family of adaptive immune systems that use small CRISPR RNAs (crRNAs) and CRISPR-associated (Cas) nucleases to protect prokaryotes from invading plasmids and viruses (i.e., phages).
Lucy Chou-Zheng, Asma Hatoum-Aslan
doaj +1 more source
Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli [PDF]
, 2014 Escherichia coli endoribonuclease E has a major influence on gene expression. It is essential for the maturation of ribosomal and transfer RNA as well as the rapid degradation of messenger RNA.
Clarke, JE +3 more
core +1 more source
Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement [PDF]
, 2010 Compartmentalization is an important process, since it allows the segregation of metabolic activities and, in the era of synthetic biology, represents an important tool by which defined microenvironments can be created for specific metabolic functions ...
Bhella, D. +6 more
core +2 more sources
A minimal bacterial RNase J-based degradosome is associated with translating ribosomes [PDF]
Nucleic Acids Research, 2012 Protein complexes directing messenger RNA (mRNA) degradation are present in all kingdoms of life. In Escherichia coli, mRNA degradation is performed by an RNA degradosome organized by the major ribonuclease RNase E. In bacteria lacking RNase E, the existence of a functional RNA degradosome is still an open question.
Redko, Yulia +7 more
openaire +2 more sources
mBio, 2018 Present in every kingdom of life, generally in multiple copies, DEAD-box RNA helicases are specialized enzymes that unwind RNA secondary structures. They play major roles in mRNA decay, ribosome biogenesis, and adaptation to cold temperatures.
Lamya El Mortaji +7 more
doaj +1 more source
Human RNase H1 is associated with protein P32 and is involved in mitochondrial pre-rRNA processing. [PDF]
PLoS ONE, 2013 Mammalian RNase H1 has been implicated in mitochondrial DNA replication and RNA processing and is required for embryonic development. We identified the mitochondrial protein P32 that binds specifically to human RNase H1, but not human RNase H2. P32 binds
Hongjiang Wu +4 more
doaj +1 more source
SR7 – a dual-function antisense RNA from Bacillus subtilis
RNA Biology, 2021 Here, we describe SR7, a dual-function antisense RNA encoded on the Bacillus subtilis chromosome. This RNA was earlier described as SigB-dependent regulatory RNA S1136 and reported to reduce the amount of the small ribosomal subunit under ethanol stress.
Inam Ul Haq +2 more
doaj +1 more source